Lentimicrobium saccharophilum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Lentimicrobiaceae; Lentimicrobium

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3377 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S7BZC0|A0A0S7BZC0_9BACT Nitrogen regulatory protein P-II family OS=Lentimicrobium saccharophilum OX=1678841 GN=TBC1_111758 PE=4 SV=1
LL1 pKa = 6.9IVSITFAQNAPVNFEE16 pKa = 4.07TGGYY20 pKa = 8.49GADD23 pKa = 3.46WTWTVFEE30 pKa = 5.04NGPNAPLGIITNPDD44 pKa = 2.73QSGINTSATVASFTALQSGQPWAGCEE70 pKa = 4.07SAHH73 pKa = 6.86GDD75 pKa = 3.57DD76 pKa = 4.95DD77 pKa = 5.1LGPFVLDD84 pKa = 3.54ATNSIIKK91 pKa = 10.06IMVWKK96 pKa = 10.56SVISDD101 pKa = 3.32VGIKK105 pKa = 10.0LVAASGWAQAEE116 pKa = 4.04IKK118 pKa = 10.03VANTLINQWEE128 pKa = 4.36EE129 pKa = 3.77LTFDD133 pKa = 3.84FSAYY137 pKa = 9.01PNPPGTEE144 pKa = 3.62GMYY147 pKa = 10.27DD148 pKa = 3.43QIVIFPDD155 pKa = 4.16FNLSGRR161 pKa = 11.84TQDD164 pKa = 3.2NVIYY168 pKa = 10.34FDD170 pKa = 4.81NITFNEE176 pKa = 4.24QGTSPGEE183 pKa = 3.93PTVAAPEE190 pKa = 4.14PPVRR194 pKa = 11.84EE195 pKa = 4.23PEE197 pKa = 4.07DD198 pKa = 3.63VVSVFSGAYY207 pKa = 8.22TNVAGTDD214 pKa = 4.12FNPNWGQTTVVTFPLIAGNEE234 pKa = 4.1TMKK237 pKa = 10.81YY238 pKa = 11.12ANFNYY243 pKa = 10.04QGTQFASALDD253 pKa = 3.87LSGMEE258 pKa = 4.31TLHH261 pKa = 8.63LDD263 pKa = 2.9MWTADD268 pKa = 3.25ATGVNISLISTGPVEE283 pKa = 4.13TPYY286 pKa = 11.54ALQITPNQWVSYY298 pKa = 9.07DD299 pKa = 3.32IPLTAFAGVNLADD312 pKa = 4.22VIQLKK317 pKa = 10.5FDD319 pKa = 4.19GGNGSQSIYY328 pKa = 10.5LDD330 pKa = 3.41NIYY333 pKa = 10.22FYY335 pKa = 10.95TEE337 pKa = 3.82GGVIGDD343 pKa = 3.92TPRR346 pKa = 11.84NPVDD350 pKa = 4.34FEE352 pKa = 4.5TGGYY356 pKa = 8.62GADD359 pKa = 3.46WTWTVFEE366 pKa = 5.04NGPNAPLGIIANPDD380 pKa = 2.98QSGINTSATVAAFTALQSGQPWAGCEE406 pKa = 4.07SAHH409 pKa = 6.86GDD411 pKa = 3.57DD412 pKa = 4.95DD413 pKa = 5.1LGPFVLDD420 pKa = 3.54ATNSIIKK427 pKa = 10.11IMVWKK432 pKa = 9.51PVISDD437 pKa = 3.29VGIKK441 pKa = 10.11LVANSGWAQAEE452 pKa = 3.97IKK454 pKa = 10.03VANTLINQWEE464 pKa = 4.36EE465 pKa = 3.77LTFDD469 pKa = 3.84FSAYY473 pKa = 9.01PNPPGTEE480 pKa = 3.62GMYY483 pKa = 10.27DD484 pKa = 3.43QIVIFPDD491 pKa = 4.16FNLSGRR497 pKa = 11.84TQDD500 pKa = 3.2NVIYY504 pKa = 10.34FDD506 pKa = 4.81NITFNEE512 pKa = 4.24QGTSPGEE519 pKa = 3.93PTVAAPEE526 pKa = 4.14PPVRR530 pKa = 11.84EE531 pKa = 4.23PEE533 pKa = 4.07DD534 pKa = 3.63VVSVFSGAYY543 pKa = 8.22TNVAGTDD550 pKa = 4.12FNPNWGQTTVVTFPLIAGNEE570 pKa = 4.1TMKK573 pKa = 10.81YY574 pKa = 11.12ANFNYY579 pKa = 10.04QGTQFASALDD589 pKa = 3.87LSGMEE594 pKa = 4.31TLHH597 pKa = 8.63LDD599 pKa = 2.9MWTADD604 pKa = 3.25ATGVNISLISTGPVEE619 pKa = 4.13TPYY622 pKa = 11.54ALQITPNQWVSYY634 pKa = 9.07DD635 pKa = 3.32IPLTAFAGVNLADD648 pKa = 4.22VIQLKK653 pKa = 10.5FDD655 pKa = 4.19GGNGSQSIYY664 pKa = 10.5LDD666 pKa = 3.41NIYY669 pKa = 10.22FYY671 pKa = 10.95TEE673 pKa = 3.82GGVIGDD679 pKa = 3.92TPRR682 pKa = 11.84NPVDD686 pKa = 4.34FEE688 pKa = 4.5TGGYY692 pKa = 8.62GADD695 pKa = 3.46WTWTVFEE702 pKa = 5.04NGPNAPLGIIANPDD716 pKa = 2.98QSGINTSATVAAFTALQSGQPWAGCEE742 pKa = 4.07SAHH745 pKa = 6.86GDD747 pKa = 3.57DD748 pKa = 4.95DD749 pKa = 5.1LGPFVLDD756 pKa = 3.54ATNSIIKK763 pKa = 10.11IMVWKK768 pKa = 9.51PVISDD773 pKa = 3.29VGIKK777 pKa = 10.11LVANSGWAQAEE788 pKa = 3.97IKK790 pKa = 10.03VANTLINQWEE800 pKa = 4.36EE801 pKa = 3.77LTFDD805 pKa = 3.84FSAYY809 pKa = 9.01PNPPGTEE816 pKa = 3.62GMYY819 pKa = 10.27DD820 pKa = 3.43QIVIFPDD827 pKa = 4.16FNLSGRR833 pKa = 11.84TQDD836 pKa = 3.2NVIYY840 pKa = 10.34FDD842 pKa = 4.81NITFNEE848 pKa = 4.24QGTSPGEE855 pKa = 3.93PTVAAPEE862 pKa = 4.14PPVRR866 pKa = 11.84EE867 pKa = 4.23PEE869 pKa = 4.07DD870 pKa = 3.63VVSVFSGAYY879 pKa = 8.22TNVAGTDD886 pKa = 4.12FNPNWGQTTVVTFPLIAGNEE906 pKa = 4.1TMKK909 pKa = 10.81YY910 pKa = 11.12ANFNYY915 pKa = 10.04QGTQFASALDD925 pKa = 3.87LSGMEE930 pKa = 4.31TLHH933 pKa = 8.63LDD935 pKa = 2.9MWTADD940 pKa = 3.25ATGVNISLISTGPVEE955 pKa = 4.13TPYY958 pKa = 11.54ALQITPNQWVSYY970 pKa = 9.07DD971 pKa = 3.32IPLTAFAGVNLADD984 pKa = 4.22VIQLKK989 pKa = 10.5FDD991 pKa = 4.19GGNGSQSIYY1000 pKa = 10.5LDD1002 pKa = 3.41NIYY1005 pKa = 10.22FYY1007 pKa = 10.95TEE1009 pKa = 3.82GGVIGDD1015 pKa = 3.92TPRR1018 pKa = 11.84NPVDD1022 pKa = 4.34FEE1024 pKa = 4.5TGGYY1028 pKa = 8.62GADD1031 pKa = 3.46WTWTVFEE1038 pKa = 5.04NGPNAPLGIIANPDD1052 pKa = 2.98QSGINTSATVAAFTALQSGQPWAGCEE1078 pKa = 4.07SAHH1081 pKa = 6.86GDD1083 pKa = 3.57DD1084 pKa = 4.95DD1085 pKa = 5.1LGPFVLDD1092 pKa = 3.54ATNSIIKK1099 pKa = 10.11IMVWKK1104 pKa = 9.51PVISDD1109 pKa = 3.29VGIKK1113 pKa = 10.11LVANSGWAQAEE1124 pKa = 3.97IKK1126 pKa = 10.03VANTLINQWEE1136 pKa = 4.36EE1137 pKa = 3.77LTFDD1141 pKa = 3.84FSAYY1145 pKa = 9.01PNPPGTEE1152 pKa = 3.62GMYY1155 pKa = 10.27DD1156 pKa = 3.43QIVIFPDD1163 pKa = 3.68FDD1165 pKa = 4.46LSGRR1169 pKa = 11.84TQDD1172 pKa = 3.3NVIYY1176 pKa = 10.34FDD1178 pKa = 4.81NITFNEE1184 pKa = 4.24QGTSPGEE1191 pKa = 3.93PTVAAPEE1198 pKa = 4.14PPVRR1202 pKa = 11.84EE1203 pKa = 4.23PEE1205 pKa = 4.07DD1206 pKa = 3.63VVSVFSGAYY1215 pKa = 8.22TNVAGTDD1222 pKa = 4.12FNPNWGQTTVVTFPLIAGNEE1242 pKa = 4.1TMKK1245 pKa = 10.81YY1246 pKa = 11.12ANFNYY1251 pKa = 10.04QGTQFASALDD1261 pKa = 3.87LSGMEE1266 pKa = 4.31TLHH1269 pKa = 8.63LDD1271 pKa = 2.9MWTADD1276 pKa = 3.25ATGVNISLISTGPVEE1291 pKa = 4.13TPYY1294 pKa = 11.54ALQITPNQWVSYY1306 pKa = 9.07DD1307 pKa = 3.32IPLTAFAGVNLADD1320 pKa = 4.22VIQLKK1325 pKa = 10.5FDD1327 pKa = 4.19GGNGSQSIYY1336 pKa = 10.5LDD1338 pKa = 3.41NIYY1341 pKa = 10.22FYY1343 pKa = 10.95TEE1345 pKa = 3.82GGVIGDD1351 pKa = 3.92TPRR1354 pKa = 11.84NPVDD1358 pKa = 4.34FEE1360 pKa = 4.5TGGYY1364 pKa = 8.62GADD1367 pKa = 3.46WTWTVFEE1374 pKa = 5.04NGPNAPLGIIANPDD1388 pKa = 2.98QSGINTSATVAAFTALQSGQPWAGCEE1414 pKa = 4.07SAHH1417 pKa = 6.86GDD1419 pKa = 3.57DD1420 pKa = 4.95DD1421 pKa = 5.1LGPFVLDD1428 pKa = 3.54ATNSIIKK1435 pKa = 10.11IMVWKK1440 pKa = 9.51PVISDD1445 pKa = 3.29VGIKK1449 pKa = 10.11LVANSGWAQAEE1460 pKa = 3.97IKK1462 pKa = 10.03VANTLINQWEE1472 pKa = 4.36EE1473 pKa = 3.77LTFDD1477 pKa = 3.84FSAYY1481 pKa = 9.01PNPPGTEE1488 pKa = 3.62GMYY1491 pKa = 10.27DD1492 pKa = 3.43QIVIFPDD1499 pKa = 3.68FDD1501 pKa = 4.46LSGRR1505 pKa = 11.84TQDD1508 pKa = 3.11NTIYY1512 pKa = 10.65FDD1514 pKa = 4.35NITFNEE1520 pKa = 3.95QEE1522 pKa = 4.66PIVGGPNAPVDD1533 pKa = 4.38FEE1535 pKa = 4.62TGGYY1539 pKa = 8.62GADD1542 pKa = 3.46WTWTVFEE1549 pKa = 5.04NGPNAPLGIIANPDD1563 pKa = 3.03QSGINSSATVAEE1575 pKa = 4.94FTALQSGQPWAGVEE1589 pKa = 4.26SAHH1592 pKa = 6.95GDD1594 pKa = 3.51DD1595 pKa = 4.89DD1596 pKa = 5.1LGPFVLDD1603 pKa = 3.8ATNSLIKK1610 pKa = 10.56IMVWKK1615 pKa = 9.5PVISDD1620 pKa = 3.29VGIKK1624 pKa = 10.0LVAASGWAQPEE1635 pKa = 3.96IKK1637 pKa = 10.27VANTLINQWEE1647 pKa = 4.36EE1648 pKa = 3.78LTFDD1652 pKa = 3.85FSGYY1656 pKa = 9.17PNPPGTEE1663 pKa = 3.62GMYY1666 pKa = 10.27DD1667 pKa = 3.43QIVIFPDD1674 pKa = 3.68FDD1676 pKa = 4.46LSGRR1680 pKa = 11.84TQDD1683 pKa = 3.11NTIYY1687 pKa = 10.65FDD1689 pKa = 4.35NITFNEE1695 pKa = 4.13QGTTPGEE1702 pKa = 3.97PTAAAPEE1709 pKa = 4.21PPVRR1713 pKa = 11.84EE1714 pKa = 4.23PEE1716 pKa = 4.07DD1717 pKa = 3.63VVSVFSGAYY1726 pKa = 8.22TNVAGTDD1733 pKa = 4.12FNPNWGQSTVVTFPLIAGNEE1753 pKa = 4.1TMKK1756 pKa = 10.81YY1757 pKa = 11.12ANFNYY1762 pKa = 10.04QGTQFASALDD1772 pKa = 3.87LSGMEE1777 pKa = 4.31TLHH1780 pKa = 8.63LDD1782 pKa = 2.87MWTADD1787 pKa = 3.25ASSVNVFLISTGPVEE1802 pKa = 4.13TPYY1805 pKa = 11.54ALQITPNQWVSYY1817 pKa = 9.07DD1818 pKa = 3.32IPLTAFAGVDD1828 pKa = 3.76LTDD1831 pKa = 4.32VIQMKK1836 pKa = 10.4FDD1838 pKa = 4.01GGNGSQSIYY1847 pKa = 10.5LDD1849 pKa = 3.41NIYY1852 pKa = 10.22FYY1854 pKa = 10.95TEE1856 pKa = 3.82GGVIGDD1862 pKa = 3.92TPRR1865 pKa = 11.84NPVDD1869 pKa = 4.34FEE1871 pKa = 4.5TGGYY1875 pKa = 8.62GADD1878 pKa = 3.46WTWTVFEE1885 pKa = 5.04NGPNAPLGIIANPDD1899 pKa = 2.98QSGINTSATVAEE1911 pKa = 5.03FTALQSGQPWAGVEE1925 pKa = 4.26SAHH1928 pKa = 6.95GDD1930 pKa = 3.51DD1931 pKa = 4.89DD1932 pKa = 5.1LGPFVLDD1939 pKa = 3.54ATNSIIKK1946 pKa = 10.11IMVWKK1951 pKa = 9.51PVISDD1956 pKa = 3.29VGIKK1960 pKa = 10.0LVAASGWAQPEE1971 pKa = 3.96IKK1973 pKa = 10.27VANTLINQWEE1983 pKa = 4.36EE1984 pKa = 3.78LTFDD1988 pKa = 3.85FSGYY1992 pKa = 9.17PNPPGTEE1999 pKa = 3.62GMYY2002 pKa = 10.27DD2003 pKa = 3.43QIVIFPDD2010 pKa = 3.68FDD2012 pKa = 4.46LSGRR2016 pKa = 11.84TQDD2019 pKa = 3.11NTIYY2023 pKa = 10.65FDD2025 pKa = 4.76NITFNQLVTNNDD2037 pKa = 2.66LTFPKK2042 pKa = 10.27RR2043 pKa = 11.84ILIYY2047 pKa = 9.61PNPVKK2052 pKa = 10.51RR2053 pKa = 11.84GNQIILVADD2062 pKa = 4.15VKK2064 pKa = 10.91QFEE2067 pKa = 4.75LFDD2070 pKa = 3.21ISGRR2074 pKa = 11.84VLISTNTSIVDD2085 pKa = 3.37TDD2087 pKa = 3.79KK2088 pKa = 11.12LSKK2091 pKa = 10.5GIYY2094 pKa = 8.11VLRR2097 pKa = 11.84IHH2099 pKa = 6.26TRR2101 pKa = 11.84NGDD2104 pKa = 3.12IQTQKK2109 pKa = 11.14LIVKK2113 pKa = 9.57

Molecular weight: 228.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S7BRJ5|A0A0S7BRJ5_9BACT UPF0316 protein TBC1_111338 OS=Lentimicrobium saccharophilum OX=1678841 GN=TBC1_111338 PE=3 SV=1
MM1 pKa = 7.11QLASSHH7 pKa = 6.5RR8 pKa = 11.84EE9 pKa = 3.66NILAKK14 pKa = 9.83EE15 pKa = 4.05DD16 pKa = 3.61RR17 pKa = 11.84LVVVGAARR25 pKa = 11.84PGSRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84ITAGVTVFANATINKK46 pKa = 5.83TTSYY50 pKa = 10.85LCIAPRR56 pKa = 11.84VQKK59 pKa = 10.48HH60 pKa = 5.26ALPRR64 pKa = 11.84SCANCTYY71 pKa = 10.51HH72 pKa = 7.37

Molecular weight: 7.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3377

0

3377

1291125

39

7727

382.3

42.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.512 ± 0.052

0.91 ± 0.022

5.3 ± 0.032

6.007 ± 0.052

4.961 ± 0.041

7.505 ± 0.043

1.813 ± 0.019

7.286 ± 0.039

5.425 ± 0.067

9.396 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.466 ± 0.026

5.253 ± 0.045

4.282 ± 0.034

3.202 ± 0.022

4.69 ± 0.049

6.625 ± 0.047

5.805 ± 0.086

6.315 ± 0.032

1.215 ± 0.017

4.034 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski