Passion fruit mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6KJM7|F6KJM7_9VIRU Capsid protein OS=Passion fruit mosaic virus OX=1032457 PE=3 SV=1
MM1 pKa = 8.08PYY3 pKa = 10.29QPVSLQTLPWLSANWADD20 pKa = 3.73YY21 pKa = 7.9KK22 pKa = 10.95TLLSVLRR29 pKa = 11.84ATSATSFQTQAGRR42 pKa = 11.84DD43 pKa = 3.81SIRR46 pKa = 11.84SQLAGCVSEE55 pKa = 4.54LVQVNVRR62 pKa = 11.84FPEE65 pKa = 4.59RR66 pKa = 11.84YY67 pKa = 8.8LVYY70 pKa = 11.03VNDD73 pKa = 4.45PSISDD78 pKa = 3.11VWGALLKK85 pKa = 10.38ATDD88 pKa = 3.55TKK90 pKa = 11.41NRR92 pKa = 11.84IIEE95 pKa = 4.09VDD97 pKa = 3.39NEE99 pKa = 4.15RR100 pKa = 11.84NPSNNEE106 pKa = 3.4IEE108 pKa = 4.62SVTRR112 pKa = 11.84RR113 pKa = 11.84VDD115 pKa = 3.43DD116 pKa = 4.03ASVAIRR122 pKa = 11.84INVEE126 pKa = 3.79RR127 pKa = 11.84LLKK130 pKa = 10.87LLGEE134 pKa = 4.14VHH136 pKa = 6.33GVYY139 pKa = 10.48DD140 pKa = 3.44RR141 pKa = 11.84ALFEE145 pKa = 4.32QVSGLRR151 pKa = 11.84WADD154 pKa = 3.56DD155 pKa = 3.59SAPASTSKK163 pKa = 11.13

Molecular weight: 18.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6KJM7|F6KJM7_9VIRU Capsid protein OS=Passion fruit mosaic virus OX=1032457 PE=3 SV=1
MM1 pKa = 7.74SLRR4 pKa = 11.84AQNAQDD10 pKa = 3.65VVPPLDD16 pKa = 3.58YY17 pKa = 11.12LNPSNFVKK25 pKa = 9.62TQLLGLEE32 pKa = 3.98RR33 pKa = 11.84LGLVKK38 pKa = 10.48SPKK41 pKa = 10.04KK42 pKa = 9.67MCALMEE48 pKa = 4.21TDD50 pKa = 3.63VVKK53 pKa = 10.72ISPRR57 pKa = 11.84EE58 pKa = 3.68RR59 pKa = 11.84TVFPLRR65 pKa = 11.84LTEE68 pKa = 4.48KK69 pKa = 10.51IKK71 pKa = 11.05DD72 pKa = 3.55LGNYY76 pKa = 8.82KK77 pKa = 10.45YY78 pKa = 11.55VMFLACVVSGRR89 pKa = 11.84WHH91 pKa = 6.05VPTTVKK97 pKa = 11.1GEE99 pKa = 4.21VFLSLMDD106 pKa = 3.53RR107 pKa = 11.84RR108 pKa = 11.84LTNEE112 pKa = 3.89RR113 pKa = 11.84EE114 pKa = 4.44SVILTANAKK123 pKa = 9.76PSVSEE128 pKa = 3.7FQIRR132 pKa = 11.84IHH134 pKa = 6.42PRR136 pKa = 11.84YY137 pKa = 10.88SMVSSDD143 pKa = 4.71AMQEE147 pKa = 3.88PLEE150 pKa = 4.39LFVHH154 pKa = 6.08VKK156 pKa = 9.16GLQMAEE162 pKa = 4.11GFSPLSLEE170 pKa = 3.7IAFCVVCCDD179 pKa = 3.57VVIAKK184 pKa = 9.15SLKK187 pKa = 9.78MKK189 pKa = 10.34ILQNTDD195 pKa = 2.35KK196 pKa = 10.88FGNAEE201 pKa = 4.11GEE203 pKa = 4.43VGSEE207 pKa = 4.16GLDD210 pKa = 3.56DD211 pKa = 5.37LLSGSNMPSIRR222 pKa = 11.84TSAFNSRR229 pKa = 11.84RR230 pKa = 11.84SVNANPKK237 pKa = 10.06RR238 pKa = 11.84VFKK241 pKa = 10.75GKK243 pKa = 10.07KK244 pKa = 7.95GNVWSEE250 pKa = 3.89AKK252 pKa = 10.58DD253 pKa = 3.4FAGVRR258 pKa = 11.84RR259 pKa = 11.84KK260 pKa = 10.36KK261 pKa = 10.9GVVEE265 pKa = 4.21SDD267 pKa = 3.12MLTSNAEE274 pKa = 4.28STVSNYY280 pKa = 9.28STDD283 pKa = 3.34QSDD286 pKa = 3.72ALSTGFSTDD295 pKa = 2.05TSMAVGQLGRR305 pKa = 11.84LQDD308 pKa = 3.58TSS310 pKa = 3.31

Molecular weight: 34.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3184

163

1611

796.0

90.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.151 ± 0.538

2.041 ± 0.254

6.344 ± 0.255

5.842 ± 0.16

5.119 ± 0.53

4.931 ± 0.25

1.696 ± 0.223

4.648 ± 0.407

6.376 ± 0.469

9.108 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.326

4.68 ± 0.165

3.832 ± 0.102

3.455 ± 0.131

5.842 ± 0.365

7.538 ± 0.772

6.91 ± 0.411

9.265 ± 0.37

1.225 ± 0.178

3.612 ± 0.418
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski