Rhodococcus coprophilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4163 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2X4WRT6|A0A2X4WRT6_9NOCA Beta lactamase OS=Rhodococcus coprophilus OX=38310 GN=NCTC10994_01188 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 9.81KK3 pKa = 10.05LHH5 pKa = 6.7IGAIAAVAALTVSACGSSGGDD26 pKa = 3.14ASGDD30 pKa = 3.32AAAPTGAPIVIGAQAPIKK48 pKa = 10.49GATAYY53 pKa = 8.43PQTAYY58 pKa = 10.56GLEE61 pKa = 4.29AGVHH65 pKa = 4.84YY66 pKa = 10.9VNEE69 pKa = 4.11VLGGINGRR77 pKa = 11.84PLEE80 pKa = 3.99VDD82 pKa = 3.3VCGGDD87 pKa = 3.69GSPEE91 pKa = 3.89TSINCANGFVSKK103 pKa = 10.6NVPLVIDD110 pKa = 4.71AVDD113 pKa = 3.46QSMGAAVPILGSAGIPIIGTLAGSFVADD141 pKa = 3.29AAEE144 pKa = 4.19YY145 pKa = 9.95GQAFYY150 pKa = 8.59FTGPVSFAALASMSALDD167 pKa = 3.86TMGKK171 pKa = 6.38TTASLAVNEE180 pKa = 4.97SPTSHH185 pKa = 7.02AYY187 pKa = 10.27VDD189 pKa = 4.02DD190 pKa = 5.75LIMPIADD197 pKa = 3.23GMGIDD202 pKa = 4.65VSVQYY207 pKa = 10.47PPASGANLNVVAATQLADD225 pKa = 3.76NPDD228 pKa = 3.56MAGVIALPEE237 pKa = 4.75DD238 pKa = 4.03GCTSLFQALRR248 pKa = 11.84RR249 pKa = 11.84QGYY252 pKa = 9.65EE253 pKa = 3.72DD254 pKa = 4.35TILAGSCSQFIDD266 pKa = 4.49EE267 pKa = 5.18MGTDD271 pKa = 3.31AAGIVTQPRR280 pKa = 11.84LWVPLAKK287 pKa = 10.2EE288 pKa = 3.91SAPAEE293 pKa = 4.19VQEE296 pKa = 4.23QLDD299 pKa = 3.84IFADD303 pKa = 3.44AMDD306 pKa = 3.61AVGYY310 pKa = 10.57GNEE313 pKa = 3.89LSSRR317 pKa = 11.84SIYY320 pKa = 10.13TFAAIVNIAQILSDD334 pKa = 3.73HH335 pKa = 6.53GGEE338 pKa = 3.91ITAGTVTDD346 pKa = 3.73AFKK349 pKa = 10.72AVKK352 pKa = 10.05DD353 pKa = 4.49FPSFAGPTVTCDD365 pKa = 3.08GQQWPGVATTCNAQAIFFEE384 pKa = 4.41VQEE387 pKa = 4.83DD388 pKa = 4.1GTLRR392 pKa = 11.84SVDD395 pKa = 3.38EE396 pKa = 4.49NGYY399 pKa = 10.1IDD401 pKa = 5.92LDD403 pKa = 4.03PSLMPASS410 pKa = 3.79

Molecular weight: 41.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2X4TLZ1|A0A2X4TLZ1_9NOCA Uncharacterized protein OS=Rhodococcus coprophilus OX=38310 GN=NCTC10994_00316 PE=4 SV=1
MM1 pKa = 7.47AHH3 pKa = 5.88RR4 pKa = 11.84TAIEE8 pKa = 4.21VIPAARR14 pKa = 11.84RR15 pKa = 11.84IGILALRR22 pKa = 11.84APTIGRR28 pKa = 11.84TLVPIVAPTPAPTAVPKK45 pKa = 10.32VVRR48 pKa = 11.84ISGRR52 pKa = 11.84IVVRR56 pKa = 11.84RR57 pKa = 11.84VVLIVVPRR65 pKa = 11.84VVRR68 pKa = 11.84ISVPIVVPRR77 pKa = 11.84VVLIAAPRR85 pKa = 11.84VVRR88 pKa = 11.84ISVPIVVPRR97 pKa = 11.84AVLIVVPKK105 pKa = 9.86VVRR108 pKa = 11.84TTVPIVVRR116 pKa = 11.84RR117 pKa = 11.84VVRR120 pKa = 11.84ISGRR124 pKa = 11.84IVVRR128 pKa = 11.84KK129 pKa = 9.21AVPIVVRR136 pKa = 11.84RR137 pKa = 11.84VVRR140 pKa = 11.84ITAPIVVPKK149 pKa = 10.39AVPNTGTTPVPIVVRR164 pKa = 11.84TLVPIAGLRR173 pKa = 11.84DD174 pKa = 3.33VRR176 pKa = 11.84TLVPIAAPTLARR188 pKa = 11.84IDD190 pKa = 3.56GQKK193 pKa = 9.8PRR195 pKa = 11.84RR196 pKa = 11.84RR197 pKa = 11.84TPGRR201 pKa = 11.84SRR203 pKa = 11.84TARR206 pKa = 11.84APRR209 pKa = 3.72

Molecular weight: 22.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4163

0

4163

1371707

29

7364

329.5

35.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.649 ± 0.052

0.739 ± 0.011

6.52 ± 0.033

5.813 ± 0.036

3.021 ± 0.02

9.002 ± 0.035

2.15 ± 0.02

4.032 ± 0.028

2.032 ± 0.028

9.961 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.911 ± 0.014

1.972 ± 0.02

5.706 ± 0.031

2.6 ± 0.023

7.552 ± 0.041

5.53 ± 0.022

6.195 ± 0.03

9.16 ± 0.037

1.412 ± 0.013

2.04 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski