Pepper cryptic virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Deltapartitivirus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J1DT58|A0A1J1DT58_9VIRU Putative RNA-dependent RNA polymerase OS=Pepper cryptic virus 2 OX=1050903 GN=RdRp PE=4 SV=1
MM1 pKa = 7.79AIYY4 pKa = 10.79RR5 pKa = 11.84MLNGYY10 pKa = 10.41AFTAFGNDD18 pKa = 4.26LEE20 pKa = 5.35KK21 pKa = 10.73LDD23 pKa = 3.66QRR25 pKa = 11.84HH26 pKa = 4.27IHH28 pKa = 6.55RR29 pKa = 11.84IRR31 pKa = 11.84RR32 pKa = 11.84EE33 pKa = 3.54EE34 pKa = 3.67ATTYY38 pKa = 10.64RR39 pKa = 11.84DD40 pKa = 3.18EE41 pKa = 4.53FALKK45 pKa = 10.55EE46 pKa = 3.98LMDD49 pKa = 5.19LNPILYY55 pKa = 9.57EE56 pKa = 3.8QFLEE60 pKa = 4.64GWSRR64 pKa = 11.84SYY66 pKa = 11.77YY67 pKa = 10.24EE68 pKa = 4.29GSKK71 pKa = 10.22HH72 pKa = 5.43LQAIIQYY79 pKa = 7.92GIPDD83 pKa = 4.1PHH85 pKa = 7.13PNLIDD90 pKa = 3.52KK91 pKa = 10.02EE92 pKa = 4.39IYY94 pKa = 9.89NKK96 pKa = 10.4AGYY99 pKa = 9.56VVLEE103 pKa = 4.36SLGSLPRR110 pKa = 11.84VRR112 pKa = 11.84AFDD115 pKa = 3.53VLTEE119 pKa = 4.16LDD121 pKa = 3.86SVHH124 pKa = 6.32YY125 pKa = 8.51EE126 pKa = 3.88QSSSAGCDD134 pKa = 3.13YY135 pKa = 10.74HH136 pKa = 8.59GPKK139 pKa = 10.71GPIQGEE145 pKa = 3.89NHH147 pKa = 5.33IRR149 pKa = 11.84AITRR153 pKa = 11.84AKK155 pKa = 8.72ATLWSAIKK163 pKa = 10.61DD164 pKa = 3.37EE165 pKa = 4.83GEE167 pKa = 4.44GIEE170 pKa = 4.1HH171 pKa = 7.42VIRR174 pKa = 11.84SSVPDD179 pKa = 3.2VGYY182 pKa = 8.77TRR184 pKa = 11.84TQLTDD189 pKa = 3.66LYY191 pKa = 9.69EE192 pKa = 3.99KK193 pKa = 10.34TKK195 pKa = 10.51IRR197 pKa = 11.84GVWGRR202 pKa = 11.84AFHH205 pKa = 6.67YY206 pKa = 10.13ILLEE210 pKa = 4.04GTVANPLLDD219 pKa = 3.47VFKK222 pKa = 10.96RR223 pKa = 11.84GGTFYY228 pKa = 10.77HH229 pKa = 7.19IGEE232 pKa = 4.3NPQYY236 pKa = 10.44SVPDD240 pKa = 3.41ILSQVSEE247 pKa = 4.21CCKK250 pKa = 10.48YY251 pKa = 10.65LVAIDD256 pKa = 4.01WSNFDD261 pKa = 3.1ATVARR266 pKa = 11.84FEE268 pKa = 4.61INMAFDD274 pKa = 5.15LIKK277 pKa = 10.0TLIMFPNIEE286 pKa = 4.17TEE288 pKa = 4.16LCFEE292 pKa = 4.47ICRR295 pKa = 11.84QLFIHH300 pKa = 6.58KK301 pKa = 9.99KK302 pKa = 8.94IAAPDD307 pKa = 3.67GNIYY311 pKa = 9.66WSHH314 pKa = 7.32KK315 pKa = 9.34GIPSGSYY322 pKa = 7.09FTSIIGSIVNRR333 pKa = 11.84LRR335 pKa = 11.84VEE337 pKa = 4.08YY338 pKa = 9.96IFRR341 pKa = 11.84KK342 pKa = 10.15AYY344 pKa = 9.79GVGPKK349 pKa = 8.76MCYY352 pKa = 8.05TQGDD356 pKa = 4.0DD357 pKa = 3.95SLIGADD363 pKa = 4.25FRR365 pKa = 11.84VDD367 pKa = 3.45PDD369 pKa = 3.58RR370 pKa = 11.84LSEE373 pKa = 3.97IAAPLDD379 pKa = 3.48WKK381 pKa = 10.96LNPAKK386 pKa = 9.75TDD388 pKa = 3.14VSLYY392 pKa = 9.49PEE394 pKa = 4.16NVTFLGRR401 pKa = 11.84TMYY404 pKa = 10.87GGINQRR410 pKa = 11.84DD411 pKa = 4.03LKK413 pKa = 10.81RR414 pKa = 11.84CLRR417 pKa = 11.84LLIFPEE423 pKa = 4.57FPVPSGEE430 pKa = 3.56ISAYY434 pKa = 9.3RR435 pKa = 11.84AVSIAQDD442 pKa = 3.1AGGTSEE448 pKa = 4.4VLNSIAKK455 pKa = 9.81RR456 pKa = 11.84LRR458 pKa = 11.84RR459 pKa = 11.84QYY461 pKa = 10.98GVAEE465 pKa = 4.07EE466 pKa = 4.32HH467 pKa = 6.0TVPKK471 pKa = 10.45HH472 pKa = 5.48FEE474 pKa = 4.12LYY476 pKa = 10.74VPP478 pKa = 4.68

Molecular weight: 54.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J1DT58|A0A1J1DT58_9VIRU Putative RNA-dependent RNA polymerase OS=Pepper cryptic virus 2 OX=1050903 GN=RdRp PE=4 SV=1
MM1 pKa = 7.31ATPVSDD7 pKa = 3.69TPTTKK12 pKa = 9.45STQDD16 pKa = 3.13KK17 pKa = 10.28TDD19 pKa = 3.5QLPAATPPITQKK31 pKa = 10.33PPSYY35 pKa = 9.81KK36 pKa = 10.28SEE38 pKa = 3.64ISRR41 pKa = 11.84IADD44 pKa = 3.54YY45 pKa = 10.96LADD48 pKa = 4.84DD49 pKa = 4.12YY50 pKa = 11.66EE51 pKa = 4.96IGYY54 pKa = 9.46VRR56 pKa = 11.84DD57 pKa = 3.71RR58 pKa = 11.84PPKK61 pKa = 7.61HH62 pKa = 6.13RR63 pKa = 11.84RR64 pKa = 11.84YY65 pKa = 10.28AVLRR69 pKa = 11.84TQALFNKK76 pKa = 10.12LIEE79 pKa = 4.47IYY81 pKa = 10.89GKK83 pKa = 10.22LFKK86 pKa = 10.93QNWEE90 pKa = 3.91FLRR93 pKa = 11.84RR94 pKa = 11.84SIDD97 pKa = 3.26GFTTAVGIRR106 pKa = 11.84EE107 pKa = 4.24RR108 pKa = 11.84QWEE111 pKa = 4.18NAARR115 pKa = 11.84AYY117 pKa = 9.43ISCWFFDD124 pKa = 3.95LYY126 pKa = 11.3VSVRR130 pKa = 11.84NAVEE134 pKa = 4.35KK135 pKa = 11.01VSGTAFTQYY144 pKa = 9.92YY145 pKa = 8.29HH146 pKa = 6.59AHH148 pKa = 6.6AAHH151 pKa = 7.12ILHH154 pKa = 7.08EE155 pKa = 4.27YY156 pKa = 10.23DD157 pKa = 4.0ALLVHH162 pKa = 6.82LNAIIRR168 pKa = 11.84PTHH171 pKa = 6.06IKK173 pKa = 10.3LALEE177 pKa = 3.99DD178 pKa = 4.55CIYY181 pKa = 10.29IPRR184 pKa = 11.84IATNMNITNANPFGLAEE201 pKa = 4.26ATWDD205 pKa = 3.51LDD207 pKa = 3.48ADD209 pKa = 4.36FAYY212 pKa = 11.02SIMDD216 pKa = 3.41RR217 pKa = 11.84MKK219 pKa = 10.88ASGSRR224 pKa = 11.84WRR226 pKa = 11.84VEE228 pKa = 3.77PLVTDD233 pKa = 3.69TLGRR237 pKa = 11.84PGWLFDD243 pKa = 3.24WHH245 pKa = 6.22QVGAQQQAYY254 pKa = 9.48AWFPSEE260 pKa = 4.46GNYY263 pKa = 11.0SMEE266 pKa = 4.8DD267 pKa = 3.88LIAPHH272 pKa = 7.14IIGEE276 pKa = 4.25ALTPLMGPCDD286 pKa = 3.9IDD288 pKa = 3.18DD289 pKa = 3.49WQYY292 pKa = 10.44FPNFIRR298 pKa = 11.84PANVPWQNMEE308 pKa = 3.96RR309 pKa = 11.84VSARR313 pKa = 11.84RR314 pKa = 11.84FRR316 pKa = 11.84GSAEE320 pKa = 3.47VRR322 pKa = 11.84TYY324 pKa = 11.4AEE326 pKa = 4.04EE327 pKa = 4.17KK328 pKa = 10.54VNLTRR333 pKa = 11.84FASTGHH339 pKa = 5.23EE340 pKa = 4.11TSKK343 pKa = 10.67KK344 pKa = 8.96RR345 pKa = 11.84SKK347 pKa = 10.47PSSTTTTSSQGGSRR361 pKa = 11.84DD362 pKa = 3.34MEE364 pKa = 4.46TEE366 pKa = 3.94DD367 pKa = 3.67EE368 pKa = 4.2PRR370 pKa = 11.84EE371 pKa = 4.1VVTDD375 pKa = 3.55ITLSQVTSTQQLQTLMTTGEE395 pKa = 3.97ADD397 pKa = 3.54RR398 pKa = 11.84FRR400 pKa = 11.84IIDD403 pKa = 3.52YY404 pKa = 8.86TYY406 pKa = 8.07YY407 pKa = 10.73CCVILRR413 pKa = 11.84NTAQKK418 pKa = 10.21RR419 pKa = 11.84DD420 pKa = 3.13GAIRR424 pKa = 11.84SFCFKK429 pKa = 10.54QQ430 pKa = 3.07

Molecular weight: 49.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

908

430

478

454.0

51.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.819 ± 0.848

1.432 ± 0.025

5.947 ± 0.225

6.278 ± 0.472

4.295 ± 0.074

5.947 ± 1.037

2.643 ± 0.215

7.489 ± 0.663

4.405 ± 0.307

7.599 ± 0.896

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.762 ± 0.225

3.524 ± 0.024

5.176 ± 0.117

3.744 ± 0.615

7.048 ± 0.425

6.167 ± 0.076

6.608 ± 1.512

5.176 ± 0.356

1.762 ± 0.382

5.176 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski