Myxococcus phage Mx8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Myxoctovirus; Myxococcus virus Mx8

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q94MP3|Q94MP3_9CAUD p76 OS=Myxococcus phage Mx8 OX=49964 PE=4 SV=1
MM1 pKa = 7.82TEE3 pKa = 4.17GQWCITKK10 pKa = 10.93DD11 pKa = 3.35NDD13 pKa = 3.42DD14 pKa = 3.64WMSAPGFDD22 pKa = 3.81SKK24 pKa = 11.68EE25 pKa = 3.99EE26 pKa = 3.95AVALAPKK33 pKa = 10.35LLLLSSSQKK42 pKa = 9.14FWVGRR47 pKa = 11.84AVQAASHH54 pKa = 6.66LGADD58 pKa = 4.11LVSDD62 pKa = 3.8ALEE65 pKa = 3.9NHH67 pKa = 6.86ASDD70 pKa = 4.68EE71 pKa = 4.58GPDD74 pKa = 3.72GCDD77 pKa = 3.08GSNLTTEE84 pKa = 4.03AHH86 pKa = 6.74AEE88 pKa = 3.98LEE90 pKa = 4.15ALLDD94 pKa = 3.59AWAKK98 pKa = 9.77KK99 pKa = 10.05HH100 pKa = 6.16DD101 pKa = 3.94VRR103 pKa = 11.84PLWFDD108 pKa = 3.02IEE110 pKa = 4.24EE111 pKa = 4.44EE112 pKa = 4.33EE113 pKa = 4.37EE114 pKa = 4.18HH115 pKa = 6.41VAPALHH121 pKa = 6.71LFVDD125 pKa = 4.55DD126 pKa = 5.76DD127 pKa = 4.56GNAYY131 pKa = 9.96AARR134 pKa = 11.84DD135 pKa = 3.47LDD137 pKa = 3.85HH138 pKa = 7.34AKK140 pKa = 10.31EE141 pKa = 4.03LWTADD146 pKa = 2.94TGQPAEE152 pKa = 4.61EE153 pKa = 4.5AGDD156 pKa = 3.67WQSIPDD162 pKa = 3.66EE163 pKa = 4.5RR164 pKa = 11.84EE165 pKa = 3.15ITIRR169 pKa = 11.84DD170 pKa = 3.66DD171 pKa = 3.29EE172 pKa = 4.57GTVTKK177 pKa = 10.63RR178 pKa = 11.84AGDD181 pKa = 3.54WANEE185 pKa = 3.86AFEE188 pKa = 4.8PGCIGGDD195 pKa = 3.26NYY197 pKa = 11.82

Molecular weight: 21.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q94MS9|Q94MS9_9CAUD p40 OS=Myxococcus phage Mx8 OX=49964 PE=4 SV=1
MM1 pKa = 7.62ACNPVDD7 pKa = 4.19CVVDD11 pKa = 4.12GCLRR15 pKa = 11.84PARR18 pKa = 11.84RR19 pKa = 11.84SGLCWGHH26 pKa = 6.55LKK28 pKa = 10.24RR29 pKa = 11.84RR30 pKa = 11.84QRR32 pKa = 11.84GPQGTEE38 pKa = 3.21LRR40 pKa = 11.84EE41 pKa = 3.76RR42 pKa = 11.84GLAPKK47 pKa = 10.38ARR49 pKa = 11.84FLEE52 pKa = 4.21AVYY55 pKa = 11.05DD56 pKa = 4.0LVEE59 pKa = 4.1VDD61 pKa = 5.85ASDD64 pKa = 3.31KK65 pKa = 10.95RR66 pKa = 11.84QWEE69 pKa = 4.31LAWKK73 pKa = 8.41RR74 pKa = 11.84LRR76 pKa = 11.84MAAYY80 pKa = 9.8RR81 pKa = 11.84YY82 pKa = 9.68VRR84 pKa = 11.84ADD86 pKa = 2.93KK87 pKa = 10.55VTDD90 pKa = 3.5PRR92 pKa = 11.84RR93 pKa = 11.84KK94 pKa = 9.56KK95 pKa = 10.15RR96 pKa = 11.84QSGG99 pKa = 3.34

Molecular weight: 11.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

1

86

16102

32

743

187.2

20.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.495 ± 0.479

1.565 ± 0.175

5.36 ± 0.249

6.552 ± 0.3

2.428 ± 0.164

8.042 ± 0.335

1.782 ± 0.172

2.223 ± 0.166

3.41 ± 0.22

8.738 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.174 ± 0.114

2.143 ± 0.184

6.155 ± 0.259

4.0 ± 0.328

9.024 ± 0.42

5.726 ± 0.253

5.825 ± 0.309

7.266 ± 0.259

1.95 ± 0.157

2.143 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski