Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157) (Silicibacter lacuscaerulensis)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Ruegeria; Ruegeria lacuscaerulensis

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3603 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0CU85|D0CU85_RUELI Pyridoxal phosphate homeostasis protein OS=Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157) OX=644107 GN=SL1157_1447 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 10.22KK3 pKa = 10.39VLFASTALIATASVAAAEE21 pKa = 4.58VKK23 pKa = 10.4FGGYY27 pKa = 9.98GRR29 pKa = 11.84FGLGYY34 pKa = 10.36LEE36 pKa = 5.0DD37 pKa = 3.86RR38 pKa = 11.84AVTTTTASGATNKK51 pKa = 9.62IAADD55 pKa = 3.52EE56 pKa = 4.69TILVSRR62 pKa = 11.84FRR64 pKa = 11.84LNIDD68 pKa = 4.62GIAEE72 pKa = 3.97TDD74 pKa = 3.14GGVRR78 pKa = 11.84FEE80 pKa = 4.34ARR82 pKa = 11.84VRR84 pKa = 11.84LQADD88 pKa = 3.48EE89 pKa = 4.5DD90 pKa = 4.47ASTGEE95 pKa = 4.07ANAAGLNGARR105 pKa = 11.84FSVIYY110 pKa = 10.46GGLRR114 pKa = 11.84VDD116 pKa = 4.02AGNVAGAFDD125 pKa = 4.34NLAGYY130 pKa = 9.35YY131 pKa = 10.42GNEE134 pKa = 4.03PGLEE138 pKa = 4.13TFIGQYY144 pKa = 10.54SGVDD148 pKa = 3.67YY149 pKa = 11.39DD150 pKa = 4.32FLAYY154 pKa = 10.4DD155 pKa = 3.79STGSGANAVFFQYY168 pKa = 11.24AVGDD172 pKa = 3.88FAFGASYY179 pKa = 10.63DD180 pKa = 3.74QNTTYY185 pKa = 11.21AAGVATDD192 pKa = 3.59ADD194 pKa = 3.8RR195 pKa = 11.84WDD197 pKa = 3.28ISATYY202 pKa = 9.72TFGNITAALAYY213 pKa = 9.58GQTDD217 pKa = 3.71GGAGSDD223 pKa = 3.56PSLTVLTLGGEE234 pKa = 4.34FGDD237 pKa = 4.09LSGTLFVADD246 pKa = 4.88DD247 pKa = 3.97ATEE250 pKa = 3.99VAATDD255 pKa = 3.53GTAYY259 pKa = 10.44GLSLAYY265 pKa = 10.47NLGAATTLTFAYY277 pKa = 10.15GDD279 pKa = 3.76GSADD283 pKa = 4.06EE284 pKa = 4.72DD285 pKa = 4.06TQNYY289 pKa = 9.51GIGAIYY295 pKa = 10.27DD296 pKa = 3.84LGGGASLRR304 pKa = 11.84GGIGVSDD311 pKa = 4.81CDD313 pKa = 3.56VCADD317 pKa = 3.43STLRR321 pKa = 11.84ADD323 pKa = 5.63FGAQFNFF330 pKa = 3.8

Molecular weight: 33.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0CSX3|D0CSX3_RUELI Glycosyl transferase group 2 family OS=Ruegeria lacuscaerulensis (strain DSM 11314 / KCTC 2953 / ITI-1157) OX=644107 GN=SL1157_3246 PE=4 SV=1
MM1 pKa = 6.98TPKK4 pKa = 9.43TGRR7 pKa = 11.84WAALKK12 pKa = 10.34AQLRR16 pKa = 11.84PALARR21 pKa = 11.84IRR23 pKa = 11.84QKK25 pKa = 10.58VPRR28 pKa = 11.84GLRR31 pKa = 11.84FVVGILLIIGGIFGFLPVVGFWMIPLGIMVAAMDD65 pKa = 3.5VQLFVRR71 pKa = 11.84WLRR74 pKa = 11.84KK75 pKa = 9.06RR76 pKa = 11.84RR77 pKa = 11.84QGPRR81 pKa = 11.84RR82 pKa = 3.51

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3603

0

3603

1059865

31

1510

294.2

32.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.945 ± 0.053

0.948 ± 0.013

6.093 ± 0.034

5.894 ± 0.039

3.735 ± 0.027

8.484 ± 0.038

2.105 ± 0.022

5.157 ± 0.03

3.369 ± 0.034

9.92 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.77 ± 0.018

2.655 ± 0.022

5.219 ± 0.028

3.488 ± 0.023

6.84 ± 0.042

4.973 ± 0.026

5.298 ± 0.028

7.275 ± 0.033

1.44 ± 0.018

2.26 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski