Nostoc sp. 3335mG

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Nostoc; unclassified Nostoc

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8647 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A318BD35|A0A318BD35_9NOSO Uncharacterized protein OS=Nostoc sp. 3335mG OX=2204170 GN=DMC47_14130 PE=4 SV=1
MM1 pKa = 7.39TKK3 pKa = 10.38LAKK6 pKa = 10.01TGDD9 pKa = 3.7GVSRR13 pKa = 11.84RR14 pKa = 11.84LLSGTALASAAACALIATPVYY35 pKa = 10.57AQDD38 pKa = 3.33EE39 pKa = 4.7CGPTTEE45 pKa = 4.46GTVTCTAANNPYY57 pKa = 9.97PNGIEE62 pKa = 4.11YY63 pKa = 7.6STPAVDD69 pKa = 4.02PLQDD73 pKa = 4.23PGLDD77 pKa = 3.67PNAPVYY83 pKa = 10.89DD84 pKa = 3.66LTVNLGSGVAIDD96 pKa = 4.9ADD98 pKa = 3.64ATRR101 pKa = 11.84PGVALIGFNDD111 pKa = 4.21GAVTLNSFGDD121 pKa = 3.54TSITVTGVGAQGVLASTYY139 pKa = 10.22TGDD142 pKa = 3.61LTISTDD148 pKa = 3.47SITATGGASGGINANSSAGTITINADD174 pKa = 3.43TTSVSGNGSAGISANSFAGNVDD196 pKa = 2.86ITAGTVTAEE205 pKa = 4.26GLASSGIVAYY215 pKa = 10.67SNQGDD220 pKa = 3.77VTINAGTVTTTADD233 pKa = 2.91GGYY236 pKa = 10.45FGINSAAIEE245 pKa = 4.08AVSVQGTTNVTVDD258 pKa = 3.49SVSTTGVYY266 pKa = 10.19AAGISAYY273 pKa = 10.36SGVSANVTADD283 pKa = 3.83TISTTGYY290 pKa = 9.63ASYY293 pKa = 10.84GAIVQSRR300 pKa = 11.84GDD302 pKa = 3.22ASLTVGSISTAGDD315 pKa = 3.56YY316 pKa = 9.42STGAFVQTLNGTASLTSTGSITTTGDD342 pKa = 3.08DD343 pKa = 4.12AIGVFVDD350 pKa = 4.59SVAGNATVVVNDD362 pKa = 3.56VSTTGDD368 pKa = 3.02NSTAVYY374 pKa = 10.88ADD376 pKa = 3.49GDD378 pKa = 4.06IVDD381 pKa = 3.71VTINGDD387 pKa = 3.15VSTQGLASFGVDD399 pKa = 2.89AFGTDD404 pKa = 3.17TVTVTNNGTITTAGDD419 pKa = 3.46GSIGVLVTGVNGAVVDD435 pKa = 4.45GSGSVSTSGDD445 pKa = 3.01NSRR448 pKa = 11.84GLSIRR453 pKa = 11.84SVFGDD458 pKa = 3.23ADD460 pKa = 3.8VTVGDD465 pKa = 4.03VTTTGANSAAVVATSDD481 pKa = 3.7FGNVSVTNTGAIDD494 pKa = 3.65TSGDD498 pKa = 2.99ASAGIRR504 pKa = 11.84AVAFTGNATVANNGTVTTTGLGSTGIYY531 pKa = 9.86AGAYY535 pKa = 9.79GDD537 pKa = 3.82VTVTGAGSVASSGVGIEE554 pKa = 4.41AYY556 pKa = 10.27SVAGSIDD563 pKa = 3.34ITHH566 pKa = 6.95GDD568 pKa = 3.07IATTEE573 pKa = 4.41DD574 pKa = 3.74GASGIVAEE582 pKa = 4.48TGNYY586 pKa = 9.8NYY588 pKa = 10.82AAGNITIDD596 pKa = 3.56VGDD599 pKa = 3.65ITTTGDD605 pKa = 3.25NADD608 pKa = 4.88GINASALSGGTIDD621 pKa = 3.86ITHH624 pKa = 6.78GAITTSGTNAFGVFAIGVDD643 pKa = 3.76DD644 pKa = 4.21VSISGTSATTTGEE657 pKa = 4.06DD658 pKa = 3.11AAGVYY663 pKa = 9.81GVSLFGDD670 pKa = 3.48VTIDD674 pKa = 3.14VGTVSTAGEE683 pKa = 4.13YY684 pKa = 10.98APGVVGAALLGGDD697 pKa = 3.73VSITAGTITTTGDD710 pKa = 3.38TSKK713 pKa = 11.0GAYY716 pKa = 9.2GLSRR720 pKa = 11.84YY721 pKa = 9.63GSTAITVGNVTTSGDD736 pKa = 3.45YY737 pKa = 10.99SIGVDD742 pKa = 3.37AYY744 pKa = 9.63AYY746 pKa = 9.34YY747 pKa = 10.3GASVTVNGTVATSGDD762 pKa = 3.84YY763 pKa = 11.04APGVNVYY770 pKa = 10.55AVGLGGTDD778 pKa = 2.8VDD780 pKa = 4.3NNATVVNNGAIRR792 pKa = 11.84TSGIEE797 pKa = 4.1SNGITAEE804 pKa = 4.04AVFGDD809 pKa = 4.01VVISGTGSVATTGDD823 pKa = 3.58FSTGILARR831 pKa = 11.84SANGVVDD838 pKa = 3.73ITAGTVTTTGIASGIDD854 pKa = 3.41AYY856 pKa = 10.91ASGGVSITVDD866 pKa = 3.42TVRR869 pKa = 11.84TAGADD874 pKa = 3.5SNAINAGGFYY884 pKa = 10.64SVDD887 pKa = 3.09VTANTVSTTGASANGIDD904 pKa = 3.45AVSFGGDD911 pKa = 3.22VTVAAGSVIASGADD925 pKa = 3.22STAIRR930 pKa = 11.84ALGFGGGANVSVTGAVRR947 pKa = 11.84STSGTAIDD955 pKa = 4.21ILAAGAGGAGGPAVGDD971 pKa = 3.69PANDD975 pKa = 4.74GIARR979 pKa = 11.84VLVGTNGSVQGGTNAITTDD998 pKa = 3.88ALNGTQITNRR1008 pKa = 11.84GSIIGGSGYY1017 pKa = 10.64AIEE1020 pKa = 4.71ASGGAATINNIGSITGRR1037 pKa = 11.84LLLTDD1042 pKa = 4.01NSDD1045 pKa = 3.43TLTNSGNFTLIGNSDD1060 pKa = 3.57FGGGNDD1066 pKa = 4.27LLTNSGTVRR1075 pKa = 11.84FGSAAGTQNITLVGLEE1091 pKa = 4.11SFANSGLVDD1100 pKa = 4.11LRR1102 pKa = 11.84NGVAGDD1108 pKa = 3.86RR1109 pKa = 11.84LTLAAYY1115 pKa = 7.59TGSGPAALGLDD1126 pKa = 3.39IAFGATPTVDD1136 pKa = 2.96RR1137 pKa = 11.84LNVTTATGSTSVVLNPLNGPATLIPTTTLIQASAASSATAFTLSAGSQFNGLIQYY1192 pKa = 9.62GLVYY1196 pKa = 10.67NPTSFAYY1203 pKa = 10.24QLISAPNATVYY1214 pKa = 10.42RR1215 pKa = 11.84QAKK1218 pKa = 8.73LGEE1221 pKa = 4.78GISTLWNRR1229 pKa = 11.84SADD1232 pKa = 3.81AITAHH1237 pKa = 6.94LAADD1241 pKa = 4.44RR1242 pKa = 11.84DD1243 pKa = 4.6SGWSAPTADD1252 pKa = 3.24SAGRR1256 pKa = 11.84IWLQMFGEE1264 pKa = 4.42VNKK1267 pKa = 10.32RR1268 pKa = 11.84DD1269 pKa = 3.57EE1270 pKa = 4.23TRR1272 pKa = 11.84SFGFNGLAQNDD1283 pKa = 3.06VDD1285 pKa = 5.45LGYY1288 pKa = 10.57KK1289 pKa = 9.77QDD1291 pKa = 3.46VFGGQIGFDD1300 pKa = 3.61LYY1302 pKa = 10.41GTRR1305 pKa = 11.84SDD1307 pKa = 3.28TGGFTVGVSGGYY1319 pKa = 9.95ASSVMHH1325 pKa = 6.41FAGNDD1330 pKa = 3.06RR1331 pKa = 11.84FQIDD1335 pKa = 3.81AVNGTLYY1342 pKa = 10.95AGFRR1346 pKa = 11.84AGGLFLNGLAKK1357 pKa = 10.23YY1358 pKa = 10.29DD1359 pKa = 3.78YY1360 pKa = 11.46AWIKK1364 pKa = 10.53NRR1366 pKa = 11.84GNAPVSTFNLEE1377 pKa = 4.03TKK1379 pKa = 9.68AQTWGGKK1386 pKa = 9.26VEE1388 pKa = 4.01AGFRR1392 pKa = 11.84FGSNSFFIEE1401 pKa = 3.84PAASIAYY1408 pKa = 7.56TSTDD1412 pKa = 2.69MDD1414 pKa = 3.35QYY1416 pKa = 11.42GVYY1419 pKa = 10.59GGTFDD1424 pKa = 5.8FDD1426 pKa = 4.47DD1427 pKa = 3.99FTGLRR1432 pKa = 11.84GKK1434 pKa = 10.44AGARR1438 pKa = 11.84IGGSTPVGSSTLVFYY1453 pKa = 11.09AGAAAVHH1460 pKa = 5.79EE1461 pKa = 4.88FKK1463 pKa = 11.44GEE1465 pKa = 4.08DD1466 pKa = 3.39GLLFSSGGQTVRR1478 pKa = 11.84LTNDD1482 pKa = 2.82RR1483 pKa = 11.84LGTYY1487 pKa = 9.8GQGTLGLNIVTSSGVTGFIEE1507 pKa = 4.36AHH1509 pKa = 5.91GEE1511 pKa = 4.13YY1512 pKa = 10.13NDD1514 pKa = 4.11EE1515 pKa = 4.15YY1516 pKa = 11.34SGGGGRR1522 pKa = 11.84AGIRR1526 pKa = 11.84IKK1528 pKa = 10.8FF1529 pKa = 3.53

Molecular weight: 149.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A318BD78|A0A318BD78_9NOSO Ketosteroid isomerase OS=Nostoc sp. 3335mG OX=2204170 GN=DMC47_20305 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATPGGRR28 pKa = 11.84KK29 pKa = 9.14VIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84AKK41 pKa = 10.7LSAA44 pKa = 4.0

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8647

0

8647

2691358

25

2252

311.2

33.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.161 ± 0.039

0.745 ± 0.007

6.056 ± 0.021

5.216 ± 0.026

3.597 ± 0.016

8.943 ± 0.022

2.025 ± 0.015

5.058 ± 0.018

2.813 ± 0.021

9.882 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.406 ± 0.013

2.492 ± 0.019

5.395 ± 0.019

3.071 ± 0.013

7.42 ± 0.025

5.215 ± 0.018

5.461 ± 0.025

7.29 ± 0.022

1.469 ± 0.012

2.283 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski