Amino acid dipepetide frequency for Nostoc sp. 3335mG

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.527AlaAla: 18.527 ± 0.125	0.977AlaCys: 0.977 ± 0.023	7.654AlaAsp: 7.654 ± 0.057	7.467AlaGlu: 7.467 ± 0.059	4.401AlaPhe: 4.401 ± 0.043	11.531AlaGly: 11.531 ± 0.073	2.363AlaHis: 2.363 ± 0.033	7.049AlaIle: 7.049 ± 0.059	3.871AlaLys: 3.871 ± 0.05	13.914AlaLeu: 13.914 ± 0.1	3.918AlaMet: 3.918 ± 0.045	3.119AlaAsn: 3.119 ± 0.036	6.307AlaPro: 6.307 ± 0.061	4.368AlaGln: 4.368 ± 0.047	9.535AlaArg: 9.535 ± 0.071	6.572AlaSer: 6.572 ± 0.054	6.958AlaThr: 6.958 ± 0.06	8.722AlaVal: 8.722 ± 0.066	1.714AlaTrp: 1.714 ± 0.029	2.643AlaTyr: 2.643 ± 0.032	0.001AlaXaa: 0.001 ± 0.001
Cys	0.93CysAla: 0.93 ± 0.02	0.09CysCys: 0.09 ± 0.006	0.488CysAsp: 0.488 ± 0.014	0.362CysGlu: 0.362 ± 0.01	0.277CysPhe: 0.277 ± 0.011	0.809CysGly: 0.809 ± 0.018	0.198CysHis: 0.198 ± 0.009	0.331CysIle: 0.331 ± 0.012	0.138CysLys: 0.138 ± 0.008	0.678CysLeu: 0.678 ± 0.018	0.137CysMet: 0.137 ± 0.007	0.194CysAsn: 0.194 ± 0.009	0.428CysPro: 0.428 ± 0.012	0.188CysGln: 0.188 ± 0.008	0.584CysArg: 0.584 ± 0.016	0.417CysSer: 0.417 ± 0.012	0.409CysThr: 0.409 ± 0.013	0.523CysVal: 0.523 ± 0.014	0.116CysTrp: 0.116 ± 0.006	0.157CysTyr: 0.157 ± 0.007	0.0CysXaa: 0.0 ± 0.0
Asp	7.956AspAla: 7.956 ± 0.061	0.451AspCys: 0.451 ± 0.014	3.348AspAsp: 3.348 ± 0.039	3.263AspGlu: 3.263 ± 0.043	2.17AspPhe: 2.17 ± 0.028	5.782AspGly: 5.782 ± 0.052	1.355AspHis: 1.355 ± 0.027	2.966AspIle: 2.966 ± 0.039	1.608AspLys: 1.608 ± 0.026	5.864AspLeu: 5.864 ± 0.046	1.373AspMet: 1.373 ± 0.021	1.263AspAsn: 1.263 ± 0.021	3.968AspPro: 3.968 ± 0.041	1.899AspGln: 1.899 ± 0.026	5.249AspArg: 5.249 ± 0.052	2.31AspSer: 2.31 ± 0.031	2.796AspThr: 2.796 ± 0.032	4.1AspVal: 4.1 ± 0.036	1.137AspTrp: 1.137 ± 0.02	1.702AspTyr: 1.702 ± 0.025	0.0AspXaa: 0.0 ± 0.0
Glu	7.637GluAla: 7.637 ± 0.064	0.291GluCys: 0.291 ± 0.012	2.601GluAsp: 2.601 ± 0.038	2.707GluGlu: 2.707 ± 0.044	1.456GluPhe: 1.456 ± 0.021	4.365GluGly: 4.365 ± 0.041	1.13GluHis: 1.13 ± 0.024	2.811GluIle: 2.811 ± 0.032	1.773GluLys: 1.773 ± 0.028	4.854GluLeu: 4.854 ± 0.052	1.343GluMet: 1.343 ± 0.021	1.221GluAsn: 1.221 ± 0.021	2.552GluPro: 2.552 ± 0.032	1.969GluGln: 1.969 ± 0.029	4.905GluArg: 4.905 ± 0.056	2.107GluSer: 2.107 ± 0.025	3.194GluThr: 3.194 ± 0.036	3.576GluVal: 3.576 ± 0.042	0.743GluTrp: 0.743 ± 0.018	0.926GluTyr: 0.926 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	4.848PheAla: 4.848 ± 0.042	0.314PheCys: 0.314 ± 0.012	2.753PheAsp: 2.753 ± 0.035	2.003PheGlu: 2.003 ± 0.027	1.328PhePhe: 1.328 ± 0.024	3.712PheGly: 3.712 ± 0.039	0.762PheHis: 0.762 ± 0.02	1.415PheIle: 1.415 ± 0.022	0.823PheLys: 0.823 ± 0.017	3.166PheLeu: 3.166 ± 0.036	0.701PheMet: 0.701 ± 0.016	1.037PheAsn: 1.037 ± 0.024	1.519PhePro: 1.519 ± 0.026	0.913PheGln: 0.913 ± 0.018	2.332PheArg: 2.332 ± 0.033	2.05PheSer: 2.05 ± 0.029	2.052PheThr: 2.052 ± 0.031	2.778PheVal: 2.778 ± 0.034	0.54PheTrp: 0.54 ± 0.016	0.927PheTyr: 0.927 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	9.886GlyAla: 9.886 ± 0.069	0.809GlyCys: 0.809 ± 0.017	5.003GlyAsp: 5.003 ± 0.047	4.695GlyGlu: 4.695 ± 0.041	3.739GlyPhe: 3.739 ± 0.038	8.131GlyGly: 8.131 ± 0.072	1.921GlyHis: 1.921 ± 0.031	4.632GlyIle: 4.632 ± 0.047	3.099GlyLys: 3.099 ± 0.042	8.734GlyLeu: 8.734 ± 0.06	2.397GlyMet: 2.397 ± 0.033	2.321GlyAsn: 2.321 ± 0.035	3.78GlyPro: 3.78 ± 0.042	2.986GlyGln: 2.986 ± 0.031	6.662GlyArg: 6.662 ± 0.048	4.933GlySer: 4.933 ± 0.048	5.158GlyThr: 5.158 ± 0.056	6.387GlyVal: 6.387 ± 0.048	1.725GlyTrp: 1.725 ± 0.029	2.43GlyTyr: 2.43 ± 0.028	0.001GlyXaa: 0.001 ± 0.001
His	2.442HisAla: 2.442 ± 0.031	0.192HisCys: 0.192 ± 0.008	1.321HisAsp: 1.321 ± 0.028	0.998HisGlu: 0.998 ± 0.02	0.814HisPhe: 0.814 ± 0.019	2.058HisGly: 2.058 ± 0.032	0.599HisHis: 0.599 ± 0.018	0.907HisIle: 0.907 ± 0.022	0.434HisLys: 0.434 ± 0.012	1.94HisLeu: 1.94 ± 0.029	0.463HisMet: 0.463 ± 0.015	0.441HisAsn: 0.441 ± 0.014	1.324HisPro: 1.324 ± 0.021	0.583HisGln: 0.583 ± 0.015	1.58HisArg: 1.58 ± 0.026	0.942HisSer: 0.942 ± 0.018	0.732HisThr: 0.732 ± 0.017	1.508HisVal: 1.508 ± 0.026	0.376HisTrp: 0.376 ± 0.011	0.594HisTyr: 0.594 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	7.866IleAla: 7.866 ± 0.058	0.396IleCys: 0.396 ± 0.012	4.095IleAsp: 4.095 ± 0.039	3.544IleGlu: 3.544 ± 0.039	1.601IlePhe: 1.601 ± 0.024	5.331IleGly: 5.331 ± 0.049	0.886IleHis: 0.886 ± 0.019	1.901IleIle: 1.901 ± 0.029	1.11IleLys: 1.11 ± 0.024	4.12IleLeu: 4.12 ± 0.049	0.861IleMet: 0.861 ± 0.022	1.266IleAsn: 1.266 ± 0.02	2.247IlePro: 2.247 ± 0.033	1.15IleGln: 1.15 ± 0.019	3.296IleArg: 3.296 ± 0.034	2.434IleSer: 2.434 ± 0.033	2.531IleThr: 2.531 ± 0.033	4.316IleVal: 4.316 ± 0.042	0.613IleTrp: 0.613 ± 0.014	1.013IleTyr: 1.013 ± 0.018	0.0IleXaa: 0.0 ± 0.0
Lys	3.937LysAla: 3.937 ± 0.045	0.133LysCys: 0.133 ± 0.008	1.478LysAsp: 1.478 ± 0.026	1.101LysGlu: 1.101 ± 0.021	0.803LysPhe: 0.803 ± 0.017	2.484LysGly: 2.484 ± 0.035	0.518LysHis: 0.518 ± 0.014	1.326LysIle: 1.326 ± 0.024	0.908LysLys: 0.908 ± 0.021	3.068LysLeu: 3.068 ± 0.034	0.663LysMet: 0.663 ± 0.017	0.636LysAsn: 0.636 ± 0.015	1.995LysPro: 1.995 ± 0.03	0.87LysGln: 0.87 ± 0.018	2.154LysArg: 2.154 ± 0.031	1.451LysSer: 1.451 ± 0.024	1.645LysThr: 1.645 ± 0.023	2.081LysVal: 2.081 ± 0.03	0.35LysTrp: 0.35 ± 0.012	0.528LysTyr: 0.528 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	14.014LeuAla: 14.014 ± 0.095	0.764LeuCys: 0.764 ± 0.017	6.369LeuAsp: 6.369 ± 0.053	4.374LeuGlu: 4.374 ± 0.039	3.654LeuPhe: 3.654 ± 0.046	8.386LeuGly: 8.386 ± 0.061	1.882LeuHis: 1.882 ± 0.027	4.97LeuIle: 4.97 ± 0.045	2.927LeuLys: 2.927 ± 0.032	9.441LeuLeu: 9.441 ± 0.083	2.12LeuMet: 2.12 ± 0.03	2.436LeuAsn: 2.436 ± 0.032	5.704LeuPro: 5.704 ± 0.049	2.502LeuGln: 2.502 ± 0.025	6.87LeuArg: 6.87 ± 0.056	6.024LeuSer: 6.024 ± 0.051	5.764LeuThr: 5.764 ± 0.051	7.313LeuVal: 7.313 ± 0.059	1.252LeuTrp: 1.252 ± 0.026	2.056LeuTyr: 2.056 ± 0.023	0.001LeuXaa: 0.001 ± 0.001
Met	3.25MetAla: 3.25 ± 0.034	0.151MetCys: 0.151 ± 0.008	1.089MetAsp: 1.089 ± 0.019	0.96MetGlu: 0.96 ± 0.021	0.689MetPhe: 0.689 ± 0.017	1.825MetGly: 1.825 ± 0.025	0.409MetHis: 0.409 ± 0.012	1.342MetIle: 1.342 ± 0.024	0.816MetLys: 0.816 ± 0.02	2.678MetLeu: 2.678 ± 0.027	0.665MetMet: 0.665 ± 0.016	0.632MetAsn: 0.632 ± 0.016	1.526MetPro: 1.526 ± 0.023	0.707MetGln: 0.707 ± 0.016	1.816MetArg: 1.816 ± 0.031	1.459MetSer: 1.459 ± 0.023	1.817MetThr: 1.817 ± 0.023	1.715MetVal: 1.715 ± 0.027	0.234MetTrp: 0.234 ± 0.01	0.282MetTyr: 0.282 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	3.106AsnAla: 3.106 ± 0.039	0.212AsnCys: 0.212 ± 0.01	1.377AsnAsp: 1.377 ± 0.025	1.089AsnGlu: 1.089 ± 0.022	0.935AsnPhe: 0.935 ± 0.02	2.469AsnGly: 2.469 ± 0.037	0.535AsnHis: 0.535 ± 0.015	1.222AsnIle: 1.222 ± 0.024	0.596AsnLys: 0.596 ± 0.016	2.488AsnLeu: 2.488 ± 0.032	0.552AsnMet: 0.552 ± 0.016	0.673AsnAsn: 0.673 ± 0.018	1.736AsnPro: 1.736 ± 0.03	0.749AsnGln: 0.749 ± 0.016	1.822AsnArg: 1.822 ± 0.027	1.243AsnSer: 1.243 ± 0.022	1.142AsnThr: 1.142 ± 0.025	1.811AsnVal: 1.811 ± 0.032	0.431AsnTrp: 0.431 ± 0.014	0.73AsnTyr: 0.73 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	7.124ProAla: 7.124 ± 0.065	0.319ProCys: 0.319 ± 0.011	3.963ProAsp: 3.963 ± 0.04	3.472ProGlu: 3.472 ± 0.039	1.998ProPhe: 1.998 ± 0.031	4.956ProGly: 4.956 ± 0.05	1.108ProHis: 1.108 ± 0.019	2.573ProIle: 2.573 ± 0.032	1.548ProLys: 1.548 ± 0.028	4.857ProLeu: 4.857 ± 0.04	1.245ProMet: 1.245 ± 0.024	1.313ProAsn: 1.313 ± 0.025	2.763ProPro: 2.763 ± 0.041	1.705ProGln: 1.705 ± 0.023	3.194ProArg: 3.194 ± 0.039	2.741ProSer: 2.741 ± 0.036	2.716ProThr: 2.716 ± 0.038	4.427ProVal: 4.427 ± 0.041	0.73ProTrp: 0.73 ± 0.016	1.202ProTyr: 1.202 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	4.182GlnAla: 4.182 ± 0.04	0.211GlnCys: 0.211 ± 0.008	1.464GlnAsp: 1.464 ± 0.023	1.193GlnGlu: 1.193 ± 0.02	1.082GlnPhe: 1.082 ± 0.02	2.498GlnGly: 2.498 ± 0.032	0.629GlnHis: 0.629 ± 0.016	1.646GlnIle: 1.646 ± 0.025	0.851GlnLys: 0.851 ± 0.02	3.04GlnLeu: 3.04 ± 0.035	0.787GlnMet: 0.787 ± 0.015	0.778GlnAsn: 0.778 ± 0.019	1.847GlnPro: 1.847 ± 0.029	1.216GlnGln: 1.216 ± 0.026	2.528GlnArg: 2.528 ± 0.033	1.708GlnSer: 1.708 ± 0.029	1.655GlnThr: 1.655 ± 0.024	2.355GlnVal: 2.355 ± 0.033	0.425GlnTrp: 0.425 ± 0.013	0.622GlnTyr: 0.622 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	8.805ArgAla: 8.805 ± 0.075	0.509ArgCys: 0.509 ± 0.013	4.554ArgAsp: 4.554 ± 0.044	3.946ArgGlu: 3.946 ± 0.042	3.167ArgPhe: 3.167 ± 0.041	5.232ArgGly: 5.232 ± 0.047	1.748ArgHis: 1.748 ± 0.028	4.247ArgIle: 4.247 ± 0.042	1.99ArgLys: 1.99 ± 0.029	8.133ArgLeu: 8.133 ± 0.061	2.005ArgMet: 2.005 ± 0.026	1.817ArgAsn: 1.817 ± 0.026	3.833ArgPro: 3.833 ± 0.038	2.472ArgGln: 2.472 ± 0.039	5.903ArgArg: 5.903 ± 0.065	3.833ArgSer: 3.833 ± 0.044	3.742ArgThr: 3.742 ± 0.039	4.989ArgVal: 4.989 ± 0.044	1.276ArgTrp: 1.276 ± 0.025	1.996ArgTyr: 1.996 ± 0.031	0.002ArgXaa: 0.002 ± 0.001
Ser	6.402SerAla: 6.402 ± 0.056	0.383SerCys: 0.383 ± 0.011	3.13SerAsp: 3.13 ± 0.035	2.475SerGlu: 2.475 ± 0.032	2.212SerPhe: 2.212 ± 0.031	5.532SerGly: 5.532 ± 0.053	0.977SerHis: 0.977 ± 0.02	2.766SerIle: 2.766 ± 0.031	1.355SerLys: 1.355 ± 0.024	4.975SerLeu: 4.975 ± 0.053	1.175SerMet: 1.175 ± 0.019	1.387SerAsn: 1.387 ± 0.024	2.864SerPro: 2.864 ± 0.034	1.522SerGln: 1.522 ± 0.025	3.527SerArg: 3.527 ± 0.039	2.711SerSer: 2.711 ± 0.037	2.719SerThr: 2.719 ± 0.034	3.831SerVal: 3.831 ± 0.038	0.814SerTrp: 0.814 ± 0.019	1.391SerTyr: 1.391 ± 0.025	0.001SerXaa: 0.001 ± 0.001
Thr	6.583ThrAla: 6.583 ± 0.057	0.36ThrCys: 0.36 ± 0.013	3.054ThrAsp: 3.054 ± 0.037	2.374ThrGlu: 2.374 ± 0.031	1.867ThrPhe: 1.867 ± 0.028	5.504ThrGly: 5.504 ± 0.055	1.022ThrHis: 1.022 ± 0.019	3.189ThrIle: 3.189 ± 0.041	1.288ThrLys: 1.288 ± 0.022	6.122ThrLeu: 6.122 ± 0.05	1.22ThrMet: 1.22 ± 0.023	1.331ThrAsn: 1.331 ± 0.026	3.609ThrPro: 3.609 ± 0.039	1.477ThrGln: 1.477 ± 0.024	3.637ThrArg: 3.637 ± 0.039	2.773ThrSer: 2.773 ± 0.037	2.912ThrThr: 2.912 ± 0.044	4.38ThrVal: 4.38 ± 0.044	0.671ThrTrp: 0.671 ± 0.016	1.239ThrTyr: 1.239 ± 0.026	0.0ThrXaa: 0.0 ± 0.0
Val	9.87ValAla: 9.87 ± 0.063	0.538ValCys: 0.538 ± 0.015	4.435ValAsp: 4.435 ± 0.04	4.432ValGlu: 4.432 ± 0.041	2.293ValPhe: 2.293 ± 0.029	5.658ValGly: 5.658 ± 0.05	1.349ValHis: 1.349 ± 0.023	3.726ValIle: 3.726 ± 0.04	1.966ValLys: 1.966 ± 0.029	6.8ValLeu: 6.8 ± 0.051	1.664ValMet: 1.664 ± 0.024	1.915ValAsn: 1.915 ± 0.032	4.09ValPro: 4.09 ± 0.04	2.091ValGln: 2.091 ± 0.029	5.248ValArg: 5.248 ± 0.051	4.234ValSer: 4.234 ± 0.046	4.703ValThr: 4.703 ± 0.05	5.599ValVal: 5.599 ± 0.049	0.873ValTrp: 0.873 ± 0.021	1.42ValTyr: 1.42 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	1.476TrpAla: 1.476 ± 0.025	0.131TrpCys: 0.131 ± 0.007	0.753TrpAsp: 0.753 ± 0.019	0.567TrpGlu: 0.567 ± 0.015	0.584TrpPhe: 0.584 ± 0.015	0.987TrpGly: 0.987 ± 0.02	0.357TrpHis: 0.357 ± 0.012	0.722TrpIle: 0.722 ± 0.017	0.469TrpLys: 0.469 ± 0.014	1.749TrpLeu: 1.749 ± 0.03	0.364TrpMet: 0.364 ± 0.012	0.495TrpAsn: 0.495 ± 0.015	0.747TrpPro: 0.747 ± 0.019	0.622TrpGln: 0.622 ± 0.017	1.332TrpArg: 1.332 ± 0.025	0.95TrpSer: 0.95 ± 0.021	0.905TrpThr: 0.905 ± 0.021	0.871TrpVal: 0.871 ± 0.018	0.294TrpTrp: 0.294 ± 0.012	0.311TrpTyr: 0.311 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.77TyrAla: 2.77 ± 0.031	0.223TyrCys: 0.223 ± 0.009	1.633TyrAsp: 1.633 ± 0.033	1.139TyrGlu: 1.139 ± 0.023	0.899TyrPhe: 0.899 ± 0.019	2.18TyrGly: 2.18 ± 0.029	0.501TyrHis: 0.501 ± 0.013	0.837TyrIle: 0.837 ± 0.021	0.559TyrLys: 0.559 ± 0.015	2.201TyrLeu: 2.201 ± 0.028	0.409TyrMet: 0.409 ± 0.011	0.643TyrAsn: 0.643 ± 0.016	1.113TyrPro: 1.113 ± 0.022	0.725TyrGln: 0.725 ± 0.019	2.024TyrArg: 2.024 ± 0.031	1.258TyrSer: 1.258 ± 0.023	1.059TyrThr: 1.059 ± 0.022	1.62TyrVal: 1.62 ± 0.022	0.372TyrTrp: 0.372 ± 0.013	0.661TyrTyr: 0.661 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.011XaaXaa: 0.011 ± 0.003

Statistics based on 8647 proteins (2691358 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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