Vibrio phage VSKK

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Fibrovirus; Vibrio virus fs1; Vibrio phage VSK

Average proteome isoelectric point is 7.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8W6E1|Q8W6E1_9VIRU Putative major coat protein OS=Vibrio phage VSKK OX=180503 PE=4 SV=1
MM1 pKa = 7.29NEE3 pKa = 4.71DD4 pKa = 3.45MNEE7 pKa = 3.73LLTRR11 pKa = 11.84LNSDD15 pKa = 3.45NNKK18 pKa = 9.66QLDD21 pKa = 3.95DD22 pKa = 4.12VNNQLLQLNTQSQRR36 pKa = 11.84IVAQIAKK43 pKa = 9.05QEE45 pKa = 4.11KK46 pKa = 8.44QDD48 pKa = 3.42AAIYY52 pKa = 9.41EE53 pKa = 4.23NTKK56 pKa = 11.01ALIQNLNKK64 pKa = 10.62DD65 pKa = 3.06VTTAVNKK72 pKa = 6.75TTNAVNALGSKK83 pKa = 10.33VDD85 pKa = 3.84GLSDD89 pKa = 3.48AVDD92 pKa = 3.62GLGEE96 pKa = 4.32DD97 pKa = 3.59VSAIKK102 pKa = 10.5DD103 pKa = 3.59AITNVDD109 pKa = 3.12TSGAGISGTCIEE121 pKa = 5.16SDD123 pKa = 3.5TCTGFYY129 pKa = 10.59EE130 pKa = 4.24SGYY133 pKa = 9.37PDD135 pKa = 5.32GISGIFSQHH144 pKa = 5.97FEE146 pKa = 4.26TVSEE150 pKa = 4.52SVHH153 pKa = 6.21DD154 pKa = 3.94TVKK157 pKa = 11.2DD158 pKa = 3.93FMKK161 pKa = 10.12IDD163 pKa = 3.92LSHH166 pKa = 6.56AQRR169 pKa = 11.84PSFSIPVLHH178 pKa = 7.03FGKK181 pKa = 10.26FQLL184 pKa = 4.45

Molecular weight: 19.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8W6D9|Q8W6D9_9VIRU Zot-like protein OS=Vibrio phage VSKK OX=180503 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 10.33ADD4 pKa = 5.87LINKK8 pKa = 8.86RR9 pKa = 11.84PKK11 pKa = 10.11RR12 pKa = 11.84YY13 pKa = 8.59STMFLNLVFSKK24 pKa = 10.67KK25 pKa = 10.12LPQAIRR31 pKa = 11.84RR32 pKa = 11.84TGQRR36 pKa = 11.84ASVGQVSRR44 pKa = 11.84RR45 pKa = 11.84EE46 pKa = 3.83WQPRR50 pKa = 11.84RR51 pKa = 11.84DD52 pKa = 3.42

Molecular weight: 6.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1205

52

411

200.8

22.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.722 ± 1.071

1.577 ± 0.366

5.809 ± 0.748

4.979 ± 0.556

4.896 ± 0.302

5.809 ± 0.557

2.656 ± 0.61

7.718 ± 1.182

6.722 ± 0.311

7.718 ± 0.925

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.905 ± 0.573

4.481 ± 0.785

4.647 ± 0.567

4.398 ± 0.545

5.062 ± 1.065

6.473 ± 1.053

5.809 ± 0.482

6.473 ± 0.377

1.162 ± 0.314

3.983 ± 1.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski