Blochmannia endosymbiont of Polyrhachis (Hedomyrma) turneri

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Enterobacteriaceae incertae sedis; ant, tsetse, mealybug, aphid, etc. endosymbionts; ant endosymbionts; Candidatus Blochmannia; unclassified Candidatus Blochmannia endosymbionts

Average proteome isoelectric point is 7.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 589 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3U3G6|A0A0E3U3G6_9ENTR Asparagine--tRNA ligase OS=Blochmannia endosymbiont of Polyrhachis (Hedomyrma) turneri OX=1505596 GN=asnS PE=3 SV=1
MM1 pKa = 7.07STIEE5 pKa = 4.57DD6 pKa = 3.49RR7 pKa = 11.84VKK9 pKa = 11.08VIIAEE14 pKa = 3.86QLGVKK19 pKa = 10.16KK20 pKa = 10.6EE21 pKa = 3.96DD22 pKa = 4.08VINHH26 pKa = 6.41ASFVEE31 pKa = 3.97DD32 pKa = 4.72LGADD36 pKa = 3.39SLDD39 pKa = 3.64TVEE42 pKa = 5.9LVMALEE48 pKa = 4.53EE49 pKa = 4.22EE50 pKa = 4.81FNTEE54 pKa = 3.82IPDD57 pKa = 3.56EE58 pKa = 4.17EE59 pKa = 4.47AEE61 pKa = 5.03KK62 pKa = 9.69ITTVQAAINFIQANQSAINSS82 pKa = 3.84

Molecular weight: 9.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3U3P8|A0A0E3U3P8_9ENTR 50S ribosomal protein L13 OS=Blochmannia endosymbiont of Polyrhachis (Hedomyrma) turneri OX=1505596 GN=rplM PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.63RR12 pKa = 11.84IRR14 pKa = 11.84THH16 pKa = 5.65GFRR19 pKa = 11.84ARR21 pKa = 11.84MATSNGRR28 pKa = 11.84KK29 pKa = 8.56ILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.74GRR39 pKa = 11.84IKK41 pKa = 10.21LTATKK46 pKa = 10.18KK47 pKa = 9.73NHH49 pKa = 5.39

Molecular weight: 5.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

589

0

589

198143

38

1425

336.4

38.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.911 ± 0.081

1.695 ± 0.041

4.582 ± 0.071

4.681 ± 0.081

4.727 ± 0.08

5.956 ± 0.113

2.649 ± 0.05

10.589 ± 0.121

6.753 ± 0.097

10.153 ± 0.1

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.039

6.481 ± 0.107

3.202 ± 0.05

3.659 ± 0.063

4.437 ± 0.09

6.988 ± 0.076

4.93 ± 0.085

6.182 ± 0.124

0.999 ± 0.035

3.959 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski