Marmoricola solisilvae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Marmoricola

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N0DTX5|A0A3N0DTX5_9ACTN Uncharacterized protein OS=Marmoricola solisilvae OX=1542737 GN=EFL95_08545 PE=4 SV=1
MM1 pKa = 7.34TLDD4 pKa = 3.64SDD6 pKa = 4.22WYY8 pKa = 10.58AVRR11 pKa = 11.84CVFRR15 pKa = 11.84SIVEE19 pKa = 4.26VGVTTYY25 pKa = 9.95EE26 pKa = 3.71EE27 pKa = 6.05RR28 pKa = 11.84ITLWQAASTDD38 pKa = 3.75EE39 pKa = 4.72AIEE42 pKa = 3.97RR43 pKa = 11.84AEE45 pKa = 4.29AEE47 pKa = 4.15AEE49 pKa = 4.12EE50 pKa = 4.18YY51 pKa = 10.74AAAIEE56 pKa = 4.25DD57 pKa = 3.99AEE59 pKa = 4.12IEE61 pKa = 4.5YY62 pKa = 10.94VGLAQCFHH70 pKa = 6.88LFDD73 pKa = 5.56DD74 pKa = 4.97PVDD77 pKa = 3.7GAEE80 pKa = 4.38IFSLMRR86 pKa = 11.84DD87 pKa = 3.23SDD89 pKa = 4.79LDD91 pKa = 3.82PEE93 pKa = 5.2DD94 pKa = 4.2YY95 pKa = 11.31LDD97 pKa = 4.66AFFDD101 pKa = 4.0TGDD104 pKa = 3.35EE105 pKa = 4.37HH106 pKa = 7.78QSHH109 pKa = 6.89GDD111 pKa = 3.45DD112 pKa = 3.41

Molecular weight: 12.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N0E0J4|A0A3N0E0J4_9ACTN TetR/AcrR family transcriptional regulator OS=Marmoricola solisilvae OX=1542737 GN=EFL95_03255 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.51LLKK22 pKa = 8.15KK23 pKa = 9.12TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84LGKK33 pKa = 10.04

Molecular weight: 4.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3726

0

3726

1174687

33

3197

315.3

33.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.598 ± 0.053

0.754 ± 0.012

6.18 ± 0.034

5.582 ± 0.047

3.036 ± 0.024

9.189 ± 0.04

2.129 ± 0.019

4.06 ± 0.027

2.663 ± 0.035

10.01 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.889 ± 0.017

2.313 ± 0.034

5.397 ± 0.028

2.846 ± 0.02

6.704 ± 0.056

5.711 ± 0.037

6.395 ± 0.05

9.017 ± 0.033

1.45 ± 0.019

2.075 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski