Clostridium phage phi3626

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8SBL9|Q8SBL9_9CAUD DNA helicase OS=Clostridium phage phi3626 OX=190478 PE=3 SV=1
MM1 pKa = 7.66CEE3 pKa = 4.38LYY5 pKa = 11.1EE6 pKa = 4.39NMLNEE11 pKa = 4.41ILSKK15 pKa = 10.82QYY17 pKa = 10.46DD18 pKa = 3.32ICKK21 pKa = 10.54DD22 pKa = 2.74KK23 pKa = 10.83WNCLDD28 pKa = 4.82CIFEE32 pKa = 4.19NEE34 pKa = 3.96CDD36 pKa = 3.55KK37 pKa = 11.43VFVSDD42 pKa = 5.15RR43 pKa = 11.84PDD45 pKa = 3.9SICDD49 pKa = 3.12KK50 pKa = 9.8EE51 pKa = 4.54TFIKK55 pKa = 10.21KK56 pKa = 9.74IKK58 pKa = 10.22EE59 pKa = 3.74EE60 pKa = 3.89MLRR63 pKa = 11.84GCEE66 pKa = 3.82

Molecular weight: 7.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8SBL3|Q8SBL3_9CAUD Gp41 protein OS=Clostridium phage phi3626 OX=190478 PE=4 SV=1
MM1 pKa = 7.53YY2 pKa = 9.88KK3 pKa = 10.04RR4 pKa = 11.84KK5 pKa = 9.62YY6 pKa = 10.09KK7 pKa = 10.35SRR9 pKa = 11.84QVEE12 pKa = 3.85IRR14 pKa = 11.84VLNVVDD20 pKa = 4.23YY21 pKa = 10.91IINNKK26 pKa = 6.02VTIRR30 pKa = 11.84EE31 pKa = 3.91AAKK34 pKa = 10.3VFGVSKK40 pKa = 9.66STVYY44 pKa = 10.87LDD46 pKa = 3.18STSRR50 pKa = 11.84ILEE53 pKa = 4.09INPQKK58 pKa = 11.13AMEE61 pKa = 4.13VEE63 pKa = 4.88KK64 pKa = 10.6IILQNKK70 pKa = 7.56SKK72 pKa = 10.81RR73 pKa = 11.84AMRR76 pKa = 11.84GVKK79 pKa = 9.36ARR81 pKa = 11.84KK82 pKa = 9.09IKK84 pKa = 10.92SLDD87 pKa = 3.36RR88 pKa = 11.84TSS90 pKa = 2.93

Molecular weight: 10.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

10313

39

983

206.3

23.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.984 ± 0.351

1.338 ± 0.226

5.537 ± 0.25

9.008 ± 0.584

4.092 ± 0.178

6.196 ± 0.561

1.096 ± 0.121

8.746 ± 0.323

10.463 ± 0.382

8.494 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.347 ± 0.151

7.282 ± 0.393

2.191 ± 0.213

2.502 ± 0.171

4.325 ± 0.375

5.808 ± 0.364

5.052 ± 0.355

5.43 ± 0.25

0.941 ± 0.126

4.169 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski