Advenella mimigardefordensis (strain DSM 17166 / LMG 22922 / DPN7)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Advenella; Advenella mimigardefordensis

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4072 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0PEU0|W0PEU0_ADVMD Transcriptional regulator LysR family OS=Advenella mimigardefordensis (strain DSM 17166 / LMG 22922 / DPN7) OX=1247726 GN=MIM_c19720 PE=3 SV=1
MM1 pKa = 6.89KK2 pKa = 10.27QWMCGPCGFIYY13 pKa = 10.43DD14 pKa = 4.35EE15 pKa = 4.45SAGLPEE21 pKa = 4.34EE22 pKa = 5.07GIPAGTRR29 pKa = 11.84FEE31 pKa = 6.1DD32 pKa = 4.92IPDD35 pKa = 3.53DD36 pKa = 4.19WVCPDD41 pKa = 4.35CGLSKK46 pKa = 10.93LDD48 pKa = 4.04FVLIPDD54 pKa = 4.06

Molecular weight: 5.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0PI75|W0PI75_ADVMD Putative ABC transporter permease protein OS=Advenella mimigardefordensis (strain DSM 17166 / LMG 22922 / DPN7) OX=1247726 GN=MIM_c34570 PE=3 SV=1
MM1 pKa = 7.22TLIDD5 pKa = 4.21PVLLGSLLVLGACTGFLAGLLGIGGGMIIVPFLTAILSTLAVPLDD50 pKa = 4.08LTVKK54 pKa = 9.63MAIATSMGTILFTSISSVRR73 pKa = 11.84AHH75 pKa = 5.51QRR77 pKa = 11.84RR78 pKa = 11.84GAIRR82 pKa = 11.84WDD84 pKa = 3.47LVRR87 pKa = 11.84AFTPGIVLGALAGSLGLFTFARR109 pKa = 11.84GTSLAILFGCFVIFSATQMFLDD131 pKa = 4.86RR132 pKa = 11.84KK133 pKa = 8.38PKK135 pKa = 9.59PSRR138 pKa = 11.84SMPEE142 pKa = 3.54LPGRR146 pKa = 11.84LGAGMMIGMISGLVGAGGGFISVPFMIWCNISMHH180 pKa = 5.62VAVATSAALGFPIALANVLGYY201 pKa = 8.49VTGGISLTHH210 pKa = 6.75LPPGSFGYY218 pKa = 9.88IWLPGLAAIALPSVLTAPLGAKK240 pKa = 8.86AAHH243 pKa = 6.03MLPVRR248 pKa = 11.84TLKK251 pKa = 10.64RR252 pKa = 11.84VFAVILYY259 pKa = 9.87LLAIYY264 pKa = 7.38MLWKK268 pKa = 10.25ALHH271 pKa = 6.01

Molecular weight: 28.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4072

0

4072

1360965

35

4309

334.2

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.848 ± 0.044

0.943 ± 0.013

5.338 ± 0.026

5.153 ± 0.033

3.737 ± 0.024

7.806 ± 0.039

2.239 ± 0.02

5.643 ± 0.025

3.814 ± 0.034

10.321 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.646 ± 0.018

3.396 ± 0.034

4.809 ± 0.028

4.424 ± 0.029

5.94 ± 0.04

6.056 ± 0.027

5.585 ± 0.029

7.343 ± 0.037

1.324 ± 0.016

2.636 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski