Pseudomonas phage phi2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U4B0L3|A0A0U4B0L3_9CAUD Uncharacterized protein OS=Pseudomonas phage phi2 OX=1450169 PE=4 SV=1
MM1 pKa = 7.29QGVDD5 pKa = 3.36PMTIIHH11 pKa = 6.39EE12 pKa = 4.28CDD14 pKa = 2.74RR15 pKa = 11.84CNATGRR21 pKa = 11.84VIEE24 pKa = 4.19TSDD27 pKa = 3.29GFRR30 pKa = 11.84CEE32 pKa = 3.77GCYY35 pKa = 10.37EE36 pKa = 3.99EE37 pKa = 5.6AQEE40 pKa = 4.2QVRR43 pKa = 11.84SEE45 pKa = 4.24ASCPEE50 pKa = 4.28CGRR53 pKa = 11.84LGVTATGICYY63 pKa = 10.38ACEE66 pKa = 3.91NTT68 pKa = 4.37

Molecular weight: 7.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U3TH05|A0A0U3TH05_9CAUD Uncharacterized protein OS=Pseudomonas phage phi2 OX=1450169 PE=4 SV=1
MM1 pKa = 7.75LKK3 pKa = 9.88HH4 pKa = 5.86QEE6 pKa = 4.07QTEE9 pKa = 4.3VLAGLLSQTALARR22 pKa = 11.84MAFAQRR28 pKa = 11.84IMAPAVAEE36 pKa = 4.47PYY38 pKa = 10.16QVVPQGRR45 pKa = 11.84GFFHH49 pKa = 7.7IVEE52 pKa = 4.33TATGKK57 pKa = 9.79VRR59 pKa = 11.84GFRR62 pKa = 11.84RR63 pKa = 11.84SHH65 pKa = 6.3NEE67 pKa = 2.79ACAYY71 pKa = 9.12AEE73 pKa = 4.03RR74 pKa = 11.84MKK76 pKa = 10.65RR77 pKa = 11.84QQAAKK82 pKa = 10.27

Molecular weight: 9.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10943

59

713

210.4

23.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.41 ± 0.612

1.087 ± 0.161

5.392 ± 0.246

6.872 ± 0.319

2.924 ± 0.213

7.768 ± 0.365

1.627 ± 0.167

4.459 ± 0.196

3.829 ± 0.346

8.59 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.188

3.162 ± 0.211

5.172 ± 0.325

4.332 ± 0.311

7.384 ± 0.49

5.967 ± 0.374

5.638 ± 0.35

6.753 ± 0.246

1.755 ± 0.184

2.431 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski