Lactococcus phage 98101

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8BLI4|A0A1P8BLI4_9CAUD HNH endonuclease OS=Lactococcus phage 98101 OX=1868854 GN=DS98101_32 PE=4 SV=1
MM1 pKa = 7.3MKK3 pKa = 8.56QTTCYY8 pKa = 10.66GCDD11 pKa = 3.3KK12 pKa = 10.25PIEE15 pKa = 4.4PEE17 pKa = 3.77WLPEE21 pKa = 4.11GEE23 pKa = 5.29FIVCDD28 pKa = 3.72EE29 pKa = 4.92CSSDD33 pKa = 3.45TDD35 pKa = 3.56KK36 pKa = 11.68LSVEE40 pKa = 4.26RR41 pKa = 11.84LQEE44 pKa = 3.9QLNTAKK50 pKa = 10.48KK51 pKa = 10.15YY52 pKa = 9.7IEE54 pKa = 4.32HH55 pKa = 7.25LIGTIKK61 pKa = 10.55HH62 pKa = 6.61DD63 pKa = 3.52GHH65 pKa = 7.76LGTIQTDD72 pKa = 4.1WILPNLEE79 pKa = 4.13KK80 pKa = 10.68ALEE83 pKa = 4.33VIGGDD88 pKa = 4.62DD89 pKa = 3.46EE90 pKa = 4.86LL91 pKa = 6.03

Molecular weight: 10.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8BLG1|A0A1P8BLG1_9CAUD Uncharacterized protein OS=Lactococcus phage 98101 OX=1868854 GN=DS98101_12 PE=4 SV=1
MM1 pKa = 7.62HH2 pKa = 7.57YY3 pKa = 9.71IPKK6 pKa = 10.05YY7 pKa = 9.07SRR9 pKa = 11.84EE10 pKa = 4.05RR11 pKa = 11.84QKK13 pKa = 10.94KK14 pKa = 8.58RR15 pKa = 11.84RR16 pKa = 11.84NNDD19 pKa = 3.27VIRR22 pKa = 11.84VTTSPFLTEE31 pKa = 3.67VHH33 pKa = 6.7SIADD37 pKa = 3.34ATVNIVRR44 pKa = 11.84NEE46 pKa = 3.75IKK48 pKa = 10.3KK49 pKa = 10.67ANYY52 pKa = 9.71QIGKK56 pKa = 9.61

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

10498

42

929

190.9

21.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.287 ± 0.322

0.724 ± 0.145

6.173 ± 0.197

7.763 ± 0.683

4.391 ± 0.178

6.315 ± 0.53

1.286 ± 0.152

6.582 ± 0.276

9.078 ± 0.474

8.03 ± 0.352

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.581 ± 0.178

6.315 ± 0.282

2.524 ± 0.169

4.296 ± 0.214

3.772 ± 0.31

6.335 ± 0.287

6.344 ± 0.307

6.068 ± 0.307

1.448 ± 0.188

3.686 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski