Microbacterium sp. CH12i

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2717 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A062VGK2|A0A062VGK2_9MICO Alanine aminopeptidase OS=Microbacterium sp. CH12i OX=1479651 GN=DC31_13595 PE=3 SV=1
MM1 pKa = 7.61NEE3 pKa = 4.03EE4 pKa = 4.1GLGDD8 pKa = 4.54EE9 pKa = 4.92IFCSLTQDD17 pKa = 4.07DD18 pKa = 4.32EE19 pKa = 5.08SGASFRR25 pKa = 11.84EE26 pKa = 4.57GFHH29 pKa = 6.61FALDD33 pKa = 3.86EE34 pKa = 4.54LDD36 pKa = 3.6LAKK39 pKa = 10.88GIDD42 pKa = 3.49VTYY45 pKa = 8.4PTGNQDD51 pKa = 3.33FVAQITQLKK60 pKa = 9.37NAGCGVVHH68 pKa = 7.0LAGTGGTLQNAAIKK82 pKa = 10.14AVQLDD87 pKa = 5.1FDD89 pKa = 4.45ATWLAPAMAVLATTATGPGAEE110 pKa = 4.36YY111 pKa = 9.66IIEE114 pKa = 4.11HH115 pKa = 5.79VRR117 pKa = 11.84VFMTGTEE124 pKa = 3.98WDD126 pKa = 3.52GDD128 pKa = 3.98QAPGQAMMQEE138 pKa = 4.55DD139 pKa = 5.15LEE141 pKa = 4.72DD142 pKa = 4.83LYY144 pKa = 10.81PDD146 pKa = 4.29YY147 pKa = 10.97IPYY150 pKa = 10.43ANSYY154 pKa = 6.17QTGYY158 pKa = 10.25LSSVTTTAILEE169 pKa = 4.07RR170 pKa = 11.84AIADD174 pKa = 3.28GDD176 pKa = 3.86LSRR179 pKa = 11.84EE180 pKa = 3.89NLMSIAASLGEE191 pKa = 4.06VDD193 pKa = 5.92DD194 pKa = 5.26LGLGGGTYY202 pKa = 10.35LYY204 pKa = 10.71GDD206 pKa = 4.61SIDD209 pKa = 3.73EE210 pKa = 4.43RR211 pKa = 11.84EE212 pKa = 4.2PTTALSVFRR221 pKa = 11.84VDD223 pKa = 3.31PAAAIGLTLEE233 pKa = 4.19KK234 pKa = 10.24FQYY237 pKa = 9.87EE238 pKa = 4.34SPLAAKK244 pKa = 10.36YY245 pKa = 9.86NAEE248 pKa = 4.0NN249 pKa = 3.48

Molecular weight: 26.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A062VSL9|A0A062VSL9_9MICO Exodeoxyribonuclease 7 small subunit OS=Microbacterium sp. CH12i OX=1479651 GN=xseB PE=3 SV=1
MM1 pKa = 7.51TKK3 pKa = 9.07RR4 pKa = 11.84TFQPNIRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.8KK16 pKa = 8.97HH17 pKa = 4.39GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84GILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.56GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2717

0

2717

784182

37

2036

288.6

31.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.911 ± 0.066

0.534 ± 0.013

6.192 ± 0.042

5.798 ± 0.042

3.166 ± 0.028

8.539 ± 0.041

2.06 ± 0.023

5.177 ± 0.037

2.312 ± 0.032

9.982 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.988 ± 0.023

2.231 ± 0.025

5.055 ± 0.035

2.95 ± 0.026

7.019 ± 0.046

5.907 ± 0.033

6.178 ± 0.033

8.543 ± 0.041

1.496 ± 0.02

1.962 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski