Bifidobacterium bohemicum DSM 22767

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium; Bifidobacterium bohemicum

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1631 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A086ZE98|A0A086ZE98_9BIFI GNAT family acetyltransferase OS=Bifidobacterium bohemicum DSM 22767 OX=1437606 GN=BBOH_1579 PE=4 SV=1
MM1 pKa = 7.8AYY3 pKa = 10.24VIAQPCVDD11 pKa = 4.41VKK13 pKa = 11.37DD14 pKa = 3.89KK15 pKa = 11.56ACVDD19 pKa = 4.0EE20 pKa = 5.6CPVDD24 pKa = 4.37CIYY27 pKa = 11.15EE28 pKa = 4.18GDD30 pKa = 3.46RR31 pKa = 11.84TLYY34 pKa = 10.34INPNEE39 pKa = 4.32CVDD42 pKa = 4.29CGACEE47 pKa = 3.97PVCPTEE53 pKa = 4.64AIFYY57 pKa = 10.04EE58 pKa = 4.75DD59 pKa = 4.8DD60 pKa = 5.52VPDD63 pKa = 3.22EE64 pKa = 4.13WAWYY68 pKa = 9.6KK69 pKa = 10.86DD70 pKa = 3.21AAVTYY75 pKa = 10.42FNDD78 pKa = 4.05LGDD81 pKa = 4.1LGGAQAAGPSGKK93 pKa = 10.15DD94 pKa = 3.22EE95 pKa = 4.12EE96 pKa = 5.62RR97 pKa = 11.84IAALPPQNQDD107 pKa = 2.49

Molecular weight: 11.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A086ZEH4|A0A086ZEH4_9BIFI CRISPR-associated protein Csd1 family OS=Bifidobacterium bohemicum DSM 22767 OX=1437606 GN=BBOH_1655 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.63KK15 pKa = 9.39KK16 pKa = 9.21HH17 pKa = 5.44RR18 pKa = 11.84KK19 pKa = 6.23MLRR22 pKa = 11.84KK23 pKa = 7.88TRR25 pKa = 11.84HH26 pKa = 3.78QRR28 pKa = 11.84KK29 pKa = 8.94

Molecular weight: 3.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1631

0

1631

569118

29

2021

348.9

38.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.351 ± 0.071

0.954 ± 0.019

6.664 ± 0.057

5.428 ± 0.062

3.451 ± 0.041

8.338 ± 0.056

2.171 ± 0.026

5.114 ± 0.046

4.072 ± 0.056

8.683 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.604 ± 0.027

3.449 ± 0.033

4.647 ± 0.036

3.555 ± 0.041

6.212 ± 0.054

6.59 ± 0.054

5.799 ± 0.046

8.011 ± 0.059

1.295 ± 0.024

2.611 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski