Gammaproteobacteria bacterium ESL0073

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; unclassified Gammaproteobacteria

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2886 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z3HYB0|A0A2Z3HYB0_9GAMM Sulfurtransferase complex subunit TusC OS=Gammaproteobacteria bacterium ESL0073 OX=2070539 GN=tusC PE=3 SV=1
MM1 pKa = 7.14PTITFLPNEE10 pKa = 4.47EE11 pKa = 4.36YY12 pKa = 10.88CPDD15 pKa = 3.6GAIVEE20 pKa = 4.42AEE22 pKa = 3.95TGEE25 pKa = 4.41TIIEE29 pKa = 3.66AAMRR33 pKa = 11.84NGIEE37 pKa = 4.06IDD39 pKa = 3.69HH40 pKa = 6.58ACEE43 pKa = 3.86MSCACTTCHH52 pKa = 6.5VIVRR56 pKa = 11.84DD57 pKa = 4.28GFDD60 pKa = 3.63SLPPSDD66 pKa = 4.68EE67 pKa = 5.37LEE69 pKa = 5.57DD70 pKa = 5.3DD71 pKa = 4.34MLDD74 pKa = 3.76KK75 pKa = 11.11AWGLEE80 pKa = 3.74PDD82 pKa = 4.3SRR84 pKa = 11.84LSCQAVVGDD93 pKa = 3.94EE94 pKa = 4.09DD95 pKa = 5.86LIVEE99 pKa = 4.56IPRR102 pKa = 11.84YY103 pKa = 8.11TINQISEE110 pKa = 3.77NHH112 pKa = 5.61

Molecular weight: 12.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z3HSZ2|A0A2Z3HSZ2_9GAMM Uncharacterized protein OS=Gammaproteobacteria bacterium ESL0073 OX=2070539 GN=DKL61_02535 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 9.76RR12 pKa = 11.84VRR14 pKa = 11.84VHH16 pKa = 6.34GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGRR28 pKa = 11.84QVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2886

0

2886

911864

29

4942

316.0

35.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.953 ± 0.048

1.106 ± 0.017

5.203 ± 0.033

5.718 ± 0.045

4.099 ± 0.033

6.647 ± 0.048

2.063 ± 0.026

7.376 ± 0.042

6.057 ± 0.044

10.587 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.302 ± 0.02

4.921 ± 0.045

3.965 ± 0.03

4.549 ± 0.041

4.186 ± 0.039

6.243 ± 0.041

5.855 ± 0.04

6.474 ± 0.038

1.232 ± 0.019

3.465 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski