alpha proteobacterium HIMB59

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Pelagibacterales; Pelagibacteraceae; unclassified Pelagibacteraceae

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1493 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9YZY3|J9YZY3_9PROT ParB-like protein OS=alpha proteobacterium HIMB59 OX=744985 GN=HIMB59_00003700 PE=3 SV=1
MM1 pKa = 7.42KK2 pKa = 10.29KK3 pKa = 9.57IAYY6 pKa = 9.6LMSAISAGLFSSAAHH21 pKa = 6.68ADD23 pKa = 3.11ISVAGSASVAYY34 pKa = 10.31NGAGGNTTIVHH45 pKa = 6.59GGSISFGLSTTTDD58 pKa = 2.72SGMTISSGAGLTRR71 pKa = 11.84DD72 pKa = 3.26TDD74 pKa = 4.17GADD77 pKa = 3.35TAPGVTGLSSVSFATGGATITAGYY101 pKa = 10.22DD102 pKa = 2.52IGLADD107 pKa = 4.3GAASVGEE114 pKa = 4.42LVTVADD120 pKa = 4.53LNSNGVTNTGGMGDD134 pKa = 4.01DD135 pKa = 3.74EE136 pKa = 4.77GAGVSLSTSFGGAALSVVYY155 pKa = 10.72VYY157 pKa = 11.19DD158 pKa = 3.72KK159 pKa = 11.28VAGTVGSGDD168 pKa = 3.95LDD170 pKa = 3.69GAANTGTSVAISMPVAGGTLGGNYY194 pKa = 9.67VSGDD198 pKa = 3.55VNGTTDD204 pKa = 3.63TEE206 pKa = 4.54SSATFSYY213 pKa = 8.26PTGAGTLTLGYY224 pKa = 10.73GSFEE228 pKa = 4.48GDD230 pKa = 2.97TTTTNGTTFGVAYY243 pKa = 11.01SMDD246 pKa = 4.16LDD248 pKa = 3.78GTAIGLGYY256 pKa = 8.12QTYY259 pKa = 10.68DD260 pKa = 3.39VNSKK264 pKa = 10.22SGNSTDD270 pKa = 3.55VTVSRR275 pKa = 11.84SLGGGASVFAEE286 pKa = 4.05MRR288 pKa = 11.84STGGTIGTTSTKK300 pKa = 10.23SSSTVAVGSSISFF313 pKa = 3.56

Molecular weight: 30.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9YYB8|J9YYB8_9PROT NOL1/NOP2/sun family protein OS=alpha proteobacterium HIMB59 OX=744985 GN=HIMB59_00003420 PE=3 SV=1
MM1 pKa = 7.47AVKK4 pKa = 10.14KK5 pKa = 10.62KK6 pKa = 10.31KK7 pKa = 9.58AAKK10 pKa = 9.88KK11 pKa = 9.73KK12 pKa = 9.92AAPKK16 pKa = 10.35KK17 pKa = 8.51KK18 pKa = 8.05TARR21 pKa = 11.84KK22 pKa = 9.03AATKK26 pKa = 10.24KK27 pKa = 10.48KK28 pKa = 10.08AVKK31 pKa = 10.18KK32 pKa = 10.17KK33 pKa = 10.18AAPKK37 pKa = 9.9KK38 pKa = 9.19AAKK41 pKa = 9.92KK42 pKa = 9.8KK43 pKa = 10.08AAPKK47 pKa = 9.87KK48 pKa = 10.42KK49 pKa = 10.15KK50 pKa = 8.93ATKK53 pKa = 10.08KK54 pKa = 9.92KK55 pKa = 8.22AAKK58 pKa = 9.71RR59 pKa = 11.84KK60 pKa = 8.43AVKK63 pKa = 10.28RR64 pKa = 11.84KK65 pKa = 8.94AAKK68 pKa = 9.97KK69 pKa = 9.59KK70 pKa = 10.03AAPKK74 pKa = 9.95KK75 pKa = 9.19AAKK78 pKa = 9.92KK79 pKa = 9.8KK80 pKa = 10.08AAPKK84 pKa = 9.78KK85 pKa = 10.28KK86 pKa = 9.82KK87 pKa = 9.8AAPKK91 pKa = 10.09RR92 pKa = 11.84KK93 pKa = 9.05AAKK96 pKa = 9.93KK97 pKa = 9.71KK98 pKa = 10.03AAPKK102 pKa = 10.19KK103 pKa = 9.88KK104 pKa = 10.05AAPKK108 pKa = 9.76KK109 pKa = 10.36KK110 pKa = 9.89KK111 pKa = 9.84AAPKK115 pKa = 9.71KK116 pKa = 10.36KK117 pKa = 9.85KK118 pKa = 9.83AAPKK122 pKa = 9.79KK123 pKa = 8.55AAKK126 pKa = 9.98RR127 pKa = 11.84KK128 pKa = 8.77AAPKK132 pKa = 9.82KK133 pKa = 8.51AAKK136 pKa = 9.91RR137 pKa = 11.84KK138 pKa = 9.02ASRR141 pKa = 11.84KK142 pKa = 9.14RR143 pKa = 11.84RR144 pKa = 3.59

Molecular weight: 15.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1493

0

1493

452782

29

1503

303.3

34.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.407 ± 0.081

0.972 ± 0.016

5.331 ± 0.052

6.073 ± 0.062

5.707 ± 0.062

6.059 ± 0.065

1.852 ± 0.029

9.456 ± 0.077

8.6 ± 0.089

9.902 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.188 ± 0.029

6.474 ± 0.063

3.349 ± 0.032

3.448 ± 0.042

3.129 ± 0.038

7.466 ± 0.052

4.602 ± 0.033

5.424 ± 0.05

0.904 ± 0.023

3.657 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski