Lysinibacillus sp. BF-4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lysinibacillus; unclassified Lysinibacillus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2407 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087N144|A0A087N144_9BACI Uncharacterized protein OS=Lysinibacillus sp. BF-4 OX=1473546 GN=CH76_10080 PE=3 SV=1
MM1 pKa = 7.69KK2 pKa = 10.4EE3 pKa = 3.38MWEE6 pKa = 4.49DD7 pKa = 3.67EE8 pKa = 4.2NSGLTIAAPTFNATKK23 pKa = 10.4VYY25 pKa = 10.91DD26 pKa = 3.94GANTVATVAAGEE38 pKa = 4.17LTGVAEE44 pKa = 4.28GDD46 pKa = 3.4IVTVNATATYY56 pKa = 11.13DD57 pKa = 3.59NADD60 pKa = 3.53VGNGKK65 pKa = 8.33TITVSYY71 pKa = 8.89TLSGADD77 pKa = 3.13AGKK80 pKa = 10.72YY81 pKa = 8.35SAPAPTLLTTGEE93 pKa = 4.11ITAKK97 pKa = 10.31ALTIAVPAVASKK109 pKa = 10.57EE110 pKa = 4.03YY111 pKa = 10.84DD112 pKa = 3.27GTTTATVTAGALTGVVGSDD131 pKa = 3.1TVTVSATGTFADD143 pKa = 4.2ANVGAGKK150 pKa = 8.0TVAVAYY156 pKa = 8.6TLAGAQAGNYY166 pKa = 7.84TLANDD171 pKa = 3.91TATADD176 pKa = 3.12ITAIAIVGAEE186 pKa = 4.51LGLTAPVIGEE196 pKa = 4.17APVTSSITGTGYY208 pKa = 9.21TGTVNWTGTLDD219 pKa = 3.77GDD221 pKa = 4.44GKK223 pKa = 10.2FQSGQVYY230 pKa = 7.77TATVTLTPATGYY242 pKa = 8.61TLTGLLEE249 pKa = 4.08NSFTNTSGGTVTNAVDD265 pKa = 3.49SATVTVTFTTLL276 pKa = 2.91

Molecular weight: 27.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087N3I5|A0A087N3I5_9BACI Membrane protein insertase YidC OS=Lysinibacillus sp. BF-4 OX=1473546 GN=yidC PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.55QPKK8 pKa = 7.97TRR10 pKa = 11.84KK11 pKa = 9.07HH12 pKa = 5.43SRR14 pKa = 11.84VHH16 pKa = 6.04GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 9.98NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2407

0

2407

713184

37

1546

296.3

33.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.005 ± 0.059

0.649 ± 0.015

5.125 ± 0.04

7.012 ± 0.054

4.285 ± 0.047

6.594 ± 0.045

2.122 ± 0.027

7.234 ± 0.044

5.975 ± 0.049

9.69 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.969 ± 0.023

3.759 ± 0.033

3.547 ± 0.03

4.313 ± 0.047

4.263 ± 0.038

5.037 ± 0.031

6.435 ± 0.038

7.646 ± 0.038

0.88 ± 0.016

3.461 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski