Wenling hepe-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KKJ2|A0A1L3KKJ2_9VIRU Putative structural protein OS=Wenling hepe-like virus 1 OX=1923493 PE=4 SV=1
MM1 pKa = 7.46SIISRR6 pKa = 11.84AQVRR10 pKa = 11.84ITNTQWSYY18 pKa = 11.55LVDD21 pKa = 3.47YY22 pKa = 10.78FNFFTVGLYY31 pKa = 10.78GLLVAYY37 pKa = 10.07LSSIAYY43 pKa = 8.88NQFTINQSIIDD54 pKa = 4.23SRR56 pKa = 11.84NMILEE61 pKa = 4.06KK62 pKa = 10.29MEE64 pKa = 4.21LQHH67 pKa = 7.0NITIDD72 pKa = 3.05IYY74 pKa = 8.99NQSAEE79 pKa = 3.81FHH81 pKa = 6.69RR82 pKa = 11.84NILEE86 pKa = 4.72AISEE90 pKa = 4.21QTSLINQTGITINEE104 pKa = 4.16NLVKK108 pKa = 10.13QHH110 pKa = 6.54NISEE114 pKa = 4.74TILEE118 pKa = 4.15QNIGAFEE125 pKa = 4.24EE126 pKa = 4.2NLVLFDD132 pKa = 6.53KK133 pKa = 10.99IILTLNDD140 pKa = 3.59TLKK143 pKa = 10.9EE144 pKa = 3.84LVSFNQSSGVNFNSVIYY161 pKa = 9.37NQEE164 pKa = 3.56RR165 pKa = 11.84VLQFLGDD172 pKa = 4.34DD173 pKa = 3.01IGKK176 pKa = 9.77YY177 pKa = 9.56VLNLLNDD184 pKa = 3.82HH185 pKa = 6.64VDD187 pKa = 3.88SILTAEE193 pKa = 4.3VNSADD198 pKa = 3.92FLGDD202 pKa = 3.22VCEE205 pKa = 4.26YY206 pKa = 11.01DD207 pKa = 3.53PAGLGPINCEE217 pKa = 3.57STQLVRR223 pKa = 11.84QRR225 pKa = 11.84MVEE228 pKa = 3.98PEE230 pKa = 3.98FQQ232 pKa = 3.2

Molecular weight: 26.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KKC2|A0A1L3KKC2_9VIRU Uncharacterized protein OS=Wenling hepe-like virus 1 OX=1923493 PE=4 SV=1
MM1 pKa = 7.39EE2 pKa = 5.15NKK4 pKa = 9.57LVKK7 pKa = 10.44LEE9 pKa = 3.96RR10 pKa = 11.84ANIIEE15 pKa = 4.2DD16 pKa = 3.81LVRR19 pKa = 11.84DD20 pKa = 3.98FDD22 pKa = 4.43FKK24 pKa = 11.55LFGKK28 pKa = 9.85QKK30 pKa = 9.07QDD32 pKa = 2.75MYY34 pKa = 11.1RR35 pKa = 11.84RR36 pKa = 11.84TIRR39 pKa = 11.84FFMEE43 pKa = 5.58GIDD46 pKa = 3.32HH47 pKa = 7.33AYY49 pKa = 8.83YY50 pKa = 10.75SRR52 pKa = 11.84WHH54 pKa = 5.1TTKK57 pKa = 10.68SDD59 pKa = 3.72AKK61 pKa = 11.06DD62 pKa = 3.35DD63 pKa = 4.02MANVLANITLPDD75 pKa = 3.51VPRR78 pKa = 11.84NPEE81 pKa = 3.88PFRR84 pKa = 11.84VTVDD88 pKa = 3.16YY89 pKa = 11.15DD90 pKa = 3.7DD91 pKa = 4.63EE92 pKa = 4.84GFHH95 pKa = 7.05IVGPNQRR102 pKa = 11.84VRR104 pKa = 11.84NVTLKK109 pKa = 10.25MNVGKK114 pKa = 10.31AISEE118 pKa = 3.89MAYY121 pKa = 10.06QSRR124 pKa = 11.84HH125 pKa = 4.73FYY127 pKa = 10.86

Molecular weight: 15.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3458

127

2419

691.6

78.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.986 ± 0.524

1.388 ± 0.423

5.928 ± 0.344

6.449 ± 0.622

4.772 ± 0.328

4.945 ± 0.3

2.371 ± 0.301

6.333 ± 0.781

7.548 ± 1.38

8.386 ± 0.681

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.298

5.639 ± 1.06

4.482 ± 0.724

4.28 ± 0.48

4.222 ± 0.379

6.709 ± 0.389

7.345 ± 0.472

6.449 ± 0.336

0.781 ± 0.194

3.702 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski