Rickettsia endosymbiont of Culicoides newsteadi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Rickettsiaceae; Rickettsieae; Rickettsia; unclassified Rickettsia

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1352 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A261DAY4|A0A261DAY4_9RICK Transposase OS=Rickettsia endosymbiont of Culicoides newsteadi OX=1961830 GN=RiCNE_07930 PE=4 SV=1
MM1 pKa = 7.49FYY3 pKa = 10.32IFSPLYY9 pKa = 10.61NIFNVLQLALNTEE22 pKa = 4.42PPLSNNFLMLFFIGNQSYY40 pKa = 7.55TAPNFYY46 pKa = 10.08IVNAGEE52 pKa = 4.35LGSIVNSEE60 pKa = 4.07EE61 pKa = 3.37FGARR65 pKa = 11.84SDD67 pKa = 3.61GATPISNRR75 pKa = 11.84RR76 pKa = 11.84ATSDD80 pKa = 2.98NVTNSSSIDD89 pKa = 3.54YY90 pKa = 10.72

Molecular weight: 9.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A261DCE2|A0A261DCE2_9RICK Uncharacterized protein OS=Rickettsia endosymbiont of Culicoides newsteadi OX=1961830 GN=RiCNE_02560 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.3RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.41GFKK19 pKa = 10.87ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84EE29 pKa = 3.88ILRR32 pKa = 11.84KK33 pKa = 9.61RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.76KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1352

0

1352

385443

31

1984

285.1

32.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.199 ± 0.074

1.13 ± 0.026

5.206 ± 0.056

5.955 ± 0.07

4.572 ± 0.067

5.463 ± 0.071

1.87 ± 0.026

9.77 ± 0.081

8.004 ± 0.073

10.143 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.299 ± 0.031

6.101 ± 0.058

3.142 ± 0.046

3.834 ± 0.047

3.616 ± 0.045

7.226 ± 0.064

5.102 ± 0.053

5.81 ± 0.058

0.812 ± 0.023

3.744 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski