Human feces smacovirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cremevirales; Smacoviridae; Porprismacovirus; Porprismacovirus humas1

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142J7I2|A0A142J7I2_9VIRU Replication associated protein OS=Human feces smacovirus 2 OX=1820158 PE=4 SV=1
MM1 pKa = 7.01FVKK4 pKa = 10.65VSEE7 pKa = 4.5TYY9 pKa = 10.75DD10 pKa = 3.33LSTKK14 pKa = 8.7VGKK17 pKa = 9.38MGMLGIHH24 pKa = 6.04TPDD27 pKa = 2.9GRR29 pKa = 11.84LVYY32 pKa = 10.92NMWKK36 pKa = 10.35GFYY39 pKa = 9.89QNYY42 pKa = 8.72RR43 pKa = 11.84KK44 pKa = 8.85MRR46 pKa = 11.84FVSCDD51 pKa = 3.03VALACASMLPADD63 pKa = 4.27PLQVGVEE70 pKa = 4.11AGDD73 pKa = 3.85IAPQDD78 pKa = 3.63MFNPILYY85 pKa = 9.77KK86 pKa = 10.6AVSNDD91 pKa = 3.59SMTLLLNRR99 pKa = 11.84IYY101 pKa = 10.98AGAHH105 pKa = 6.18KK106 pKa = 9.93EE107 pKa = 4.25SNPDD111 pKa = 3.04WVGKK115 pKa = 10.55NSVTDD120 pKa = 3.99DD121 pKa = 3.72NEE123 pKa = 4.1TSFVYY128 pKa = 10.64DD129 pKa = 2.99STRR132 pKa = 11.84EE133 pKa = 3.68VDD135 pKa = 3.53QFAMYY140 pKa = 10.44YY141 pKa = 10.68GLLSDD146 pKa = 3.69TSGWKK151 pKa = 9.93KK152 pKa = 10.98AMPQAGLSMSRR163 pKa = 11.84LRR165 pKa = 11.84PITWSLLASQGQPGVCGGFSNSVTYY190 pKa = 10.17PSDD193 pKa = 3.42GSSAGSSGNVVTSKK207 pKa = 9.33TRR209 pKa = 11.84EE210 pKa = 3.67LGRR213 pKa = 11.84FLRR216 pKa = 11.84GPACVMPAIDD226 pKa = 4.05TMVMTSDD233 pKa = 4.45GSCVNSSSEE242 pKa = 3.79QTNLVVTQAEE252 pKa = 4.33LPYY255 pKa = 10.94VNTSDD260 pKa = 3.32IQLGPGVASTVVLPNLDD277 pKa = 3.63VPDD280 pKa = 4.63CFVAAIILPPAKK292 pKa = 10.32LSRR295 pKa = 11.84LYY297 pKa = 10.97YY298 pKa = 9.4RR299 pKa = 11.84LKK301 pKa = 9.65VTWTVEE307 pKa = 4.01FFGPRR312 pKa = 11.84PLTDD316 pKa = 3.12LTNWYY321 pKa = 10.05GLALQGTQSYY331 pKa = 10.52GSDD334 pKa = 3.68YY335 pKa = 11.07VDD337 pKa = 3.4QAALITSGSTAGKK350 pKa = 9.6QAMVDD355 pKa = 3.44AGDD358 pKa = 4.21SEE360 pKa = 4.39IQKK363 pKa = 10.04VMEE366 pKa = 4.46SASS369 pKa = 3.36

Molecular weight: 39.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142J7I2|A0A142J7I2_9VIRU Replication associated protein OS=Human feces smacovirus 2 OX=1820158 PE=4 SV=1
MM1 pKa = 8.19DD2 pKa = 3.6IMATIGRR9 pKa = 11.84TGYY12 pKa = 10.62SGFAEE17 pKa = 3.65IMAIIRR23 pKa = 11.84EE24 pKa = 4.09LDD26 pKa = 2.9IHH28 pKa = 6.26KK29 pKa = 9.73WIIGAEE35 pKa = 4.12VGAGGYY41 pKa = 7.23QHH43 pKa = 6.13WQCRR47 pKa = 11.84FKK49 pKa = 10.83TRR51 pKa = 11.84LSPEE55 pKa = 3.61EE56 pKa = 4.26RR57 pKa = 11.84IEE59 pKa = 4.13GLEE62 pKa = 3.97WDD64 pKa = 4.68LKK66 pKa = 9.38TATVKK71 pKa = 10.01QKK73 pKa = 10.67KK74 pKa = 7.91RR75 pKa = 11.84TVGPAEE81 pKa = 3.89QMTEE85 pKa = 3.74KK86 pKa = 10.53LRR88 pKa = 11.84ARR90 pKa = 11.84IGDD93 pKa = 3.65GPSIYY98 pKa = 9.87TAEE101 pKa = 4.86CSDD104 pKa = 2.93NWEE107 pKa = 4.19YY108 pKa = 10.45EE109 pKa = 4.26AKK111 pKa = 10.34EE112 pKa = 4.05GAYY115 pKa = 9.87LASWDD120 pKa = 3.8TMKK123 pKa = 10.36VRR125 pKa = 11.84STRR128 pKa = 11.84FGKK131 pKa = 9.73PSWQQEE137 pKa = 4.04VALQALRR144 pKa = 11.84DD145 pKa = 3.87TNDD148 pKa = 3.24RR149 pKa = 11.84QVVVWYY155 pKa = 9.73DD156 pKa = 3.32PQGNIGKK163 pKa = 8.92SWLTNHH169 pKa = 7.47LYY171 pKa = 10.28EE172 pKa = 5.34QGLAYY177 pKa = 9.93CIPATMNNIEE187 pKa = 4.64GIIKK191 pKa = 8.85TVASLAVKK199 pKa = 10.12DD200 pKa = 4.25RR201 pKa = 11.84DD202 pKa = 3.15MGYY205 pKa = 9.56PPRR208 pKa = 11.84PYY210 pKa = 10.55VIIDD214 pKa = 3.66IPRR217 pKa = 11.84SWKK220 pKa = 9.68WSEE223 pKa = 3.9QLYY226 pKa = 8.31TAIEE230 pKa = 4.32SIKK233 pKa = 10.66DD234 pKa = 3.62GLIMDD239 pKa = 5.0PRR241 pKa = 11.84YY242 pKa = 9.77SAQPINIRR250 pKa = 11.84GVKK253 pKa = 9.21VMVMTNTMPKK263 pKa = 8.96IDD265 pKa = 4.66KK266 pKa = 10.18LSSDD270 pKa = 3.03RR271 pKa = 11.84WVIHH275 pKa = 5.71TNPP278 pKa = 3.61

Molecular weight: 31.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

647

278

369

323.5

35.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.419 ± 0.12

1.391 ± 0.167

6.182 ± 0.228

4.482 ± 1.064

2.164 ± 0.579

7.883 ± 0.368

0.927 ± 0.273

5.719 ± 1.941

5.1 ± 0.734

7.264 ± 0.998

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.173 ± 0.116

3.709 ± 0.252

5.255 ± 0.117

4.173 ± 0.077

4.791 ± 0.899

8.346 ± 1.576

6.646 ± 0.091

7.419 ± 1.081

2.628 ± 0.71

4.328 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski