Duck hepatitis B virus (isolate Shanghai/DHBVQCA34) (DHBV)

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Avihepadnavirus; Duck hepatitis B virus

Average proteome isoelectric point is 8.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q66406|CAPSD_DHBVQ Capsid protein OS=Duck hepatitis B virus (isolate Shanghai/DHBVQCA34) OX=644639 GN=C PE=3 SV=1
MM1 pKa = 7.15GQQPAKK7 pKa = 10.78SMDD10 pKa = 2.92VRR12 pKa = 11.84RR13 pKa = 11.84IEE15 pKa = 4.69GGEE18 pKa = 3.94LLLNQLAGRR27 pKa = 11.84MIPKK31 pKa = 8.84GTVTWSGKK39 pKa = 10.16FPTIDD44 pKa = 3.24HH45 pKa = 6.83LLDD48 pKa = 3.59HH49 pKa = 6.24VQTMEE54 pKa = 4.49EE55 pKa = 4.67VNTLQQQGAWPAGAGRR71 pKa = 11.84RR72 pKa = 11.84LGLTNPTPHH81 pKa = 6.63EE82 pKa = 4.3TPQPQWTPEE91 pKa = 3.83EE92 pKa = 4.01DD93 pKa = 3.23QKK95 pKa = 11.68ARR97 pKa = 11.84EE98 pKa = 3.95AFRR101 pKa = 11.84RR102 pKa = 11.84YY103 pKa = 8.96QEE105 pKa = 3.91EE106 pKa = 4.36RR107 pKa = 11.84PPEE110 pKa = 4.12TTTIAPTSPTPWKK123 pKa = 9.68LQPGDD128 pKa = 4.75DD129 pKa = 3.94PLLEE133 pKa = 4.35NKK135 pKa = 10.1SLLEE139 pKa = 3.89THH141 pKa = 7.35PLYY144 pKa = 10.71QNPEE148 pKa = 3.83PAVPVIKK155 pKa = 9.85TPPLKK160 pKa = 10.1KK161 pKa = 10.13KK162 pKa = 10.46KK163 pKa = 9.72MPGTFGGILAGLIGLLVSFFLLIKK187 pKa = 10.07ILEE190 pKa = 4.06ILRR193 pKa = 11.84RR194 pKa = 11.84LDD196 pKa = 3.1WWWISLSSPKK206 pKa = 10.95GKK208 pKa = 8.31MQCAFQDD215 pKa = 3.05TGAQISQHH223 pKa = 5.34YY224 pKa = 9.84VGSCPWGCPGFLWTYY239 pKa = 10.39LRR241 pKa = 11.84LFIIFLLILLVAAGLLYY258 pKa = 10.12LTDD261 pKa = 3.4NMSIILEE268 pKa = 4.07KK269 pKa = 10.68LQWEE273 pKa = 4.62SVSVLFSSISSLLPSDD289 pKa = 3.65QKK291 pKa = 11.76SLVALMFGLLLIWMTSSSATQTLVTLTQLATLSVLFYY328 pKa = 11.28KK329 pKa = 10.78NN330 pKa = 2.82

Molecular weight: 36.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q66405|HBSAG_DHBVQ Large envelope protein OS=Duck hepatitis B virus (isolate Shanghai/DHBVQCA34) OX=644639 GN=S PE=3 SV=1
MM1 pKa = 7.45PQPLKK6 pKa = 10.84QSLDD10 pKa = 3.17QSKK13 pKa = 10.49RR14 pKa = 11.84LRR16 pKa = 11.84EE17 pKa = 4.21AEE19 pKa = 3.75KK20 pKa = 10.54HH21 pKa = 5.62LRR23 pKa = 11.84EE24 pKa = 4.61LEE26 pKa = 4.05NLVDD30 pKa = 4.46SNLEE34 pKa = 3.97EE35 pKa = 4.51EE36 pKa = 4.37KK37 pKa = 10.94LKK39 pKa = 10.52PQLSMGEE46 pKa = 4.42DD47 pKa = 3.39VQSPGKK53 pKa = 10.64GEE55 pKa = 3.85PLHH58 pKa = 6.29PNVRR62 pKa = 11.84APLSHH67 pKa = 6.3VVRR70 pKa = 11.84AATIDD75 pKa = 3.69LPRR78 pKa = 11.84LGNKK82 pKa = 9.66LPAKK86 pKa = 9.73HH87 pKa = 6.56HH88 pKa = 6.15LGKK91 pKa = 10.87LSGLYY96 pKa = 9.38QMKK99 pKa = 9.56GCTFNPEE106 pKa = 3.58WKK108 pKa = 10.59VPDD111 pKa = 3.45ISDD114 pKa = 3.37THH116 pKa = 7.31FDD118 pKa = 3.52LQVVNEE124 pKa = 4.29CPSRR128 pKa = 11.84NWKK131 pKa = 9.93YY132 pKa = 8.85LTPAKK137 pKa = 9.91FWPKK141 pKa = 9.85SISYY145 pKa = 9.65FPVQAGVKK153 pKa = 9.77AKK155 pKa = 10.91YY156 pKa = 8.9PDD158 pKa = 3.32NVMQHH163 pKa = 5.26EE164 pKa = 4.88AIVGKK169 pKa = 9.48YY170 pKa = 9.86LNRR173 pKa = 11.84LYY175 pKa = 10.71EE176 pKa = 3.99AGILYY181 pKa = 10.13KK182 pKa = 10.39RR183 pKa = 11.84ISKK186 pKa = 10.27HH187 pKa = 5.64LVTFKK192 pKa = 10.75GKK194 pKa = 9.45PYY196 pKa = 10.15NWEE199 pKa = 3.41LQYY202 pKa = 11.07LVKK205 pKa = 10.33QHH207 pKa = 5.66QVPDD211 pKa = 3.51GTTTSKK217 pKa = 10.82INGRR221 pKa = 11.84AEE223 pKa = 3.7NRR225 pKa = 11.84RR226 pKa = 11.84RR227 pKa = 11.84RR228 pKa = 11.84APAKK232 pKa = 10.28SISRR236 pKa = 11.84PHH238 pKa = 7.13DD239 pKa = 3.41SEE241 pKa = 4.98RR242 pKa = 11.84DD243 pKa = 3.71CNMVGQISNNRR254 pKa = 11.84SSIRR258 pKa = 11.84PCANNGGGKK267 pKa = 9.71HH268 pKa = 5.9SSTTGRR274 pKa = 11.84LACWGGKK281 pKa = 8.13TIGTDD286 pKa = 3.12QSYY289 pKa = 11.12SSRR292 pKa = 11.84DD293 pKa = 3.06ASASVDD299 pKa = 3.08SRR301 pKa = 11.84GRR303 pKa = 11.84SKK305 pKa = 10.88SSRR308 pKa = 11.84GFSSISRR315 pKa = 11.84RR316 pKa = 11.84KK317 pKa = 8.34ATGNHH322 pKa = 5.05HH323 pKa = 6.24HH324 pKa = 7.01CSYY327 pKa = 9.19VTNSVEE333 pKa = 3.6ATTRR337 pKa = 11.84GRR339 pKa = 11.84STPGKK344 pKa = 10.01QVFTRR349 pKa = 11.84DD350 pKa = 3.44SSSLPEE356 pKa = 3.98SRR358 pKa = 11.84ASRR361 pKa = 11.84ACDD364 pKa = 3.47KK365 pKa = 11.04DD366 pKa = 3.78SSPQEE371 pKa = 4.3EE372 pKa = 4.04EE373 pKa = 3.9NAWYY377 pKa = 10.46LRR379 pKa = 11.84GNTSWPNRR387 pKa = 11.84ITGKK391 pKa = 10.57LFLVDD396 pKa = 3.43KK397 pKa = 10.57NSRR400 pKa = 11.84NTTEE404 pKa = 3.6ARR406 pKa = 11.84LVVDD410 pKa = 5.36FSQFSKK416 pKa = 10.78GKK418 pKa = 9.03NAMRR422 pKa = 11.84FPRR425 pKa = 11.84YY426 pKa = 8.48WSPNLSTLRR435 pKa = 11.84RR436 pKa = 11.84ILPVGMPRR444 pKa = 11.84ISLDD448 pKa = 3.21LSQAFYY454 pKa = 10.9HH455 pKa = 6.6LPLNPASSSRR465 pKa = 11.84LAVSDD470 pKa = 3.95GQHH473 pKa = 4.67VYY475 pKa = 10.42YY476 pKa = 10.43FRR478 pKa = 11.84KK479 pKa = 10.17APMGVGLSPFLLHH492 pKa = 7.37LFTTALGSEE501 pKa = 3.97IARR504 pKa = 11.84RR505 pKa = 11.84FNVWTFTYY513 pKa = 9.89MDD515 pKa = 5.26DD516 pKa = 5.74FLLCHH521 pKa = 6.51PNARR525 pKa = 11.84HH526 pKa = 6.01LNSISHH532 pKa = 6.57AVCSFLQEE540 pKa = 3.6LGIRR544 pKa = 11.84INFDD548 pKa = 2.79KK549 pKa = 8.52TTPSPVNDD557 pKa = 3.02IRR559 pKa = 11.84FLGYY563 pKa = 10.64QIDD566 pKa = 3.83QKK568 pKa = 10.99FMKK571 pKa = 9.8IEE573 pKa = 3.68EE574 pKa = 4.36SRR576 pKa = 11.84WIEE579 pKa = 3.72LRR581 pKa = 11.84TVIKK585 pKa = 10.07KK586 pKa = 10.14IKK588 pKa = 9.72IGAWYY593 pKa = 9.18DD594 pKa = 3.2WKK596 pKa = 10.91CIQRR600 pKa = 11.84FVGHH604 pKa = 6.96LNFVLPFTKK613 pKa = 10.62GNIEE617 pKa = 4.08MLKK620 pKa = 10.42PMYY623 pKa = 10.41AAITNKK629 pKa = 9.7VNFSFSSAYY638 pKa = 8.34RR639 pKa = 11.84TLLYY643 pKa = 10.37KK644 pKa = 10.16LTMGVCKK651 pKa = 10.22LAIRR655 pKa = 11.84PKK657 pKa = 10.65SSVPLPRR664 pKa = 11.84VATDD668 pKa = 3.36ATPTHH673 pKa = 6.4GAISHH678 pKa = 5.63ITGGSAVFAFSKK690 pKa = 10.69VRR692 pKa = 11.84DD693 pKa = 3.38IHH695 pKa = 7.3IQEE698 pKa = 4.6LLMVCLAKK706 pKa = 10.61IMIKK710 pKa = 9.85PRR712 pKa = 11.84CILSDD717 pKa = 3.57STFVCHH723 pKa = 7.09KK724 pKa = 10.27RR725 pKa = 11.84YY726 pKa = 8.06QTLPWHH732 pKa = 6.55FAMLAKK738 pKa = 10.32QLLSPIQLYY747 pKa = 8.05FVPSKK752 pKa = 10.83YY753 pKa = 10.6NPADD757 pKa = 3.52GPSRR761 pKa = 11.84HH762 pKa = 6.15RR763 pKa = 11.84PPDD766 pKa = 3.32WTALTYY772 pKa = 10.44TPLSKK777 pKa = 10.42AIYY780 pKa = 9.36IPHH783 pKa = 7.04RR784 pKa = 11.84LCGTT788 pKa = 3.88

Molecular weight: 89.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

1

5

1852

167

788

370.4

41.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.479 ± 0.582

1.404 ± 0.261

4.158 ± 0.429

4.482 ± 0.566

3.672 ± 0.406

5.616 ± 0.468

2.916 ± 0.434

5.94 ± 0.434

5.832 ± 0.536

11.069 ± 1.56

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.89 ± 0.327

3.186 ± 0.686

6.911 ± 0.487

3.942 ± 0.553

7.127 ± 1.163

8.747 ± 0.592

6.317 ± 0.42

4.806 ± 0.25

2.43 ± 0.34

3.078 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski