Bacteroides sp. CAG:443

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; environmental samples

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2710 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6MMB2|R6MMB2_9BACE Chaperone protein ClpB OS=Bacteroides sp. CAG:443 OX=1262739 GN=clpB PE=3 SV=1
MM1 pKa = 7.46KK2 pKa = 10.48AILKK6 pKa = 8.64YY7 pKa = 10.5IKK9 pKa = 9.9GLVMIGAIISGMVVTSCEE27 pKa = 4.16DD28 pKa = 3.34EE29 pKa = 4.09PDD31 pKa = 3.31KK32 pKa = 11.88YY33 pKa = 10.77EE34 pKa = 4.6ISDD37 pKa = 3.61GLPTVKK43 pKa = 10.14YY44 pKa = 10.82IRR46 pKa = 11.84MTDD49 pKa = 3.55PEE51 pKa = 4.47VADD54 pKa = 3.76SLITGAYY61 pKa = 6.97MANTICLVGEE71 pKa = 4.19NLRR74 pKa = 11.84SVYY77 pKa = 10.24EE78 pKa = 4.66LYY80 pKa = 11.08FNDD83 pKa = 3.99QKK85 pKa = 11.66AILNTSLITDD95 pKa = 3.27NTLIVDD101 pKa = 4.35IPSTIPSVVTNKK113 pKa = 9.91IYY115 pKa = 9.74LTNKK119 pKa = 7.81NHH121 pKa = 5.61EE122 pKa = 4.38TVTYY126 pKa = 9.83DD127 pKa = 3.63FNVLVPAPEE136 pKa = 3.89ITSMSNEE143 pKa = 3.83YY144 pKa = 10.13AQPGTVATIYY154 pKa = 10.99GDD156 pKa = 3.84YY157 pKa = 10.82FIDD160 pKa = 4.6DD161 pKa = 4.4PNVPLSIEE169 pKa = 3.89IGNVPVTEE177 pKa = 4.16ITDD180 pKa = 3.64LTRR183 pKa = 11.84NAVSFVIPDD192 pKa = 3.84GAEE195 pKa = 3.21MGYY198 pKa = 10.31INVEE202 pKa = 4.36SIYY205 pKa = 10.34GTGQSTFRR213 pKa = 11.84YY214 pKa = 8.11MEE216 pKa = 4.55RR217 pKa = 11.84DD218 pKa = 3.54GILFDD223 pKa = 5.57FDD225 pKa = 6.26DD226 pKa = 5.82DD227 pKa = 5.05GDD229 pKa = 4.12DD230 pKa = 6.49AIAKK234 pKa = 10.03DD235 pKa = 4.24NGWHH239 pKa = 6.61AATIGTLDD247 pKa = 3.88KK248 pKa = 11.16VASLDD253 pKa = 3.5GNYY256 pKa = 10.6LIFQGDD262 pKa = 4.17LDD264 pKa = 3.96NGAWNDD270 pKa = 2.9TDD272 pKa = 4.49FAFEE276 pKa = 3.81YY277 pKa = 9.83WPYY280 pKa = 11.13GDD282 pKa = 4.48EE283 pKa = 3.98ATPYY287 pKa = 11.04LNTLVDD293 pKa = 5.94FEE295 pKa = 5.28DD296 pKa = 4.84LSNLQLKK303 pKa = 10.27FEE305 pKa = 4.83VNVPDD310 pKa = 3.56AWSACAMQVLLTTNNEE326 pKa = 3.85VSGNNMNNTFFQGDD340 pKa = 3.96TFPRR344 pKa = 11.84ALWIPWQSTGSYY356 pKa = 7.07TTTGWTTVTIPLSDD370 pKa = 3.65FRR372 pKa = 11.84YY373 pKa = 8.1GASGISISPLSATDD387 pKa = 3.26ITGLTLFVWNGGVTGTACSPTICIDD412 pKa = 3.59NIRR415 pKa = 11.84VVPIYY420 pKa = 10.97

Molecular weight: 46.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6NKX6|R6NKX6_9BACE 3-dehydroquinate dehydratase OS=Bacteroides sp. CAG:443 OX=1262739 GN=aroQ PE=3 SV=1
MM1 pKa = 7.0ITILRR6 pKa = 11.84IFPVFCFLQEE16 pKa = 3.8LCNRR20 pKa = 11.84LSRR23 pKa = 11.84LLLEE27 pKa = 4.81KK28 pKa = 9.79AYY30 pKa = 10.81HH31 pKa = 5.51MSRR34 pKa = 11.84FQEE37 pKa = 4.23MQTEE41 pKa = 4.74SYY43 pKa = 10.83SLYY46 pKa = 10.26RR47 pKa = 11.84KK48 pKa = 9.2SRR50 pKa = 11.84KK51 pKa = 7.2QQIIFYY57 pKa = 9.37FLQQRR62 pKa = 11.84LFQQMGLPKK71 pKa = 10.07FQTPRR76 pKa = 11.84PQPYY80 pKa = 8.93PVKK83 pKa = 10.34HH84 pKa = 6.86DD85 pKa = 3.99SLPLSQHH92 pKa = 5.45NRR94 pKa = 11.84LHH96 pKa = 5.95VPSEE100 pKa = 4.06NVLLQQLFILIGTAIQRR117 pKa = 11.84QSRR120 pKa = 11.84RR121 pKa = 11.84VNN123 pKa = 3.11

Molecular weight: 14.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2710

0

2710

996151

29

1963

367.6

41.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.121 ± 0.039

1.321 ± 0.017

5.499 ± 0.029

6.536 ± 0.041

4.488 ± 0.027

6.876 ± 0.039

1.961 ± 0.02

6.798 ± 0.038

6.468 ± 0.036

9.134 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.805 ± 0.019

5.058 ± 0.038

3.736 ± 0.022

3.611 ± 0.031

4.525 ± 0.032

6.069 ± 0.037

5.681 ± 0.031

6.513 ± 0.038

1.255 ± 0.018

4.543 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski