Microbacterium trichothecenolyticum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4075 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M2HAT0|A0A0M2HAT0_9MICO Uncharacterized protein OS=Microbacterium trichothecenolyticum OX=69370 GN=RS82_01258 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.11KK3 pKa = 10.33LRR5 pKa = 11.84VGAVAAATGVAVVLTGCASGSGGEE29 pKa = 4.29SSTGGKK35 pKa = 7.29PTIVVDD41 pKa = 3.43MWAGSEE47 pKa = 4.08ADD49 pKa = 3.48TTALEE54 pKa = 4.11EE55 pKa = 4.08QVAIAQEE62 pKa = 4.2EE63 pKa = 4.41NPDD66 pKa = 3.53VTIKK70 pKa = 10.96LQTAPWNDD78 pKa = 3.4FFTKK82 pKa = 9.7LTTNMASGNMACVTGMSGAQLGGFTDD108 pKa = 4.69GFRR111 pKa = 11.84EE112 pKa = 4.14LTAQDD117 pKa = 4.67LEE119 pKa = 4.59TAGLDD124 pKa = 3.59FADD127 pKa = 5.05FNPGAEE133 pKa = 4.81GILSFEE139 pKa = 4.36GGLYY143 pKa = 10.21GVPFDD148 pKa = 3.71VATMLVYY155 pKa = 10.71YY156 pKa = 9.69NQDD159 pKa = 2.91MLTATGAATPEE170 pKa = 4.17IGWTFDD176 pKa = 3.5DD177 pKa = 5.21FEE179 pKa = 7.65DD180 pKa = 3.5IATAATADD188 pKa = 3.51GKK190 pKa = 11.19YY191 pKa = 10.2GFGMGMGGYY200 pKa = 8.64QWMSMPIALSGKK212 pKa = 9.61QPASEE217 pKa = 5.14DD218 pKa = 3.69GTLHH222 pKa = 7.24IDD224 pKa = 4.47DD225 pKa = 5.54PDD227 pKa = 4.0FVDD230 pKa = 3.46AASWYY235 pKa = 9.86SGLVTDD241 pKa = 5.45LGVAAPVASASDD253 pKa = 3.72TGWGEE258 pKa = 3.77NQYY261 pKa = 9.94TGGNAAMAVDD271 pKa = 4.52GTWNAVSYY279 pKa = 10.88LNNEE283 pKa = 4.1SGFAAGMAPLPTGEE297 pKa = 5.1HH298 pKa = 6.43GNSGPILGSGYY309 pKa = 10.43GISKK313 pKa = 8.67TCDD316 pKa = 3.23DD317 pKa = 4.65PEE319 pKa = 5.58AALKK323 pKa = 11.1VMGSLLGKK331 pKa = 9.88DD332 pKa = 3.36AQDD335 pKa = 4.4YY336 pKa = 9.37IASSGRR342 pKa = 11.84SYY344 pKa = 10.55PARR347 pKa = 11.84TEE349 pKa = 4.03SQPLYY354 pKa = 10.21FDD356 pKa = 5.4SIDD359 pKa = 3.34EE360 pKa = 4.37EE361 pKa = 4.58YY362 pKa = 10.54RR363 pKa = 11.84DD364 pKa = 3.56QVQTVFEE371 pKa = 4.59SAFATTVPLYY381 pKa = 7.59MTKK384 pKa = 9.75NWEE387 pKa = 4.18KK388 pKa = 10.44LDD390 pKa = 5.16SYY392 pKa = 10.58IQPNLVSVYY401 pKa = 9.4NGQMEE406 pKa = 4.27MSDD409 pKa = 4.03LLEE412 pKa = 4.29SAQAQFGNN420 pKa = 3.99

Molecular weight: 44.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M2HFC5|A0A0M2HFC5_9MICO Uncharacterized protein OS=Microbacterium trichothecenolyticum OX=69370 GN=RS82_00252 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4075

0

4075

1369450

29

2346

336.1

35.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.948 ± 0.058

0.477 ± 0.008

6.437 ± 0.034

5.615 ± 0.033

3.163 ± 0.022

9.05 ± 0.032

1.959 ± 0.02

4.463 ± 0.031

1.821 ± 0.027

9.84 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.727 ± 0.015

1.877 ± 0.022

5.555 ± 0.03

2.656 ± 0.019

7.27 ± 0.049

5.433 ± 0.028

6.053 ± 0.039

9.007 ± 0.034

1.617 ± 0.018

2.031 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski