Kluyvera genomosp. 3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Kluyvera

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2234 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A248KHL4|A0A248KHL4_9ENTR Nitrate reductase molybdenum cofactor assembly chaperone OS=Kluyvera genomosp. 3 OX=2774055 GN=narJ PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84HH3 pKa = 4.86TARR6 pKa = 11.84LTLLSQCILLSLSAISGSALADD28 pKa = 3.25QTDD31 pKa = 4.06PCTGTSTTTCGFQDD45 pKa = 3.57KK46 pKa = 10.16TSTGPNGTSLIFVNNNGSAIMSDD69 pKa = 3.31AQNSNAIYY77 pKa = 10.04LWDD80 pKa = 3.53QTAGDD85 pKa = 4.12TQSLTVNGTDD95 pKa = 3.1MSGTYY100 pKa = 9.6IQGGYY105 pKa = 9.83IGTKK109 pKa = 10.42NITLNNATTDD119 pKa = 3.56MIEE122 pKa = 4.49AGNHH126 pKa = 6.46DD127 pKa = 4.67SGDD130 pKa = 3.75STNVNLAINNSTLNGEE146 pKa = 4.78DD147 pKa = 4.31DD148 pKa = 3.79STAYY152 pKa = 10.24GYY154 pKa = 11.03KK155 pKa = 9.33PAKK158 pKa = 9.52GNKK161 pKa = 9.65AYY163 pKa = 9.87MDD165 pKa = 4.22GAALFVDD172 pKa = 4.11SGSNAGTNNISIKK185 pKa = 10.62NGSSLMGSVYY195 pKa = 10.82AVTGGDD201 pKa = 3.62NNISMSDD208 pKa = 3.37SSIGGTNGSTGAIYY222 pKa = 10.88AMSNGGNNTITLEE235 pKa = 3.95NSTVVGSASEE245 pKa = 4.2PTDD248 pKa = 3.39KK249 pKa = 10.89TLLKK253 pKa = 10.61YY254 pKa = 10.84FEE256 pKa = 5.77DD257 pKa = 4.46NISGNSNASTIDD269 pKa = 3.26NLLNGSTIAMGVSGTQASSVALSNSKK295 pKa = 8.52VTGDD299 pKa = 2.97IAMVGTGSSSTASLNLSGNSNVTGDD324 pKa = 4.03ILLADD329 pKa = 4.31HH330 pKa = 6.73SAATVSMSDD339 pKa = 3.03STLTGNIDD347 pKa = 3.31ATNEE351 pKa = 3.8GNTAVALNNATVNGNITTGTGNDD374 pKa = 4.11SITLANNSHH383 pKa = 4.96VTGTVDD389 pKa = 3.47GGTGADD395 pKa = 3.95TLSLDD400 pKa = 3.98AGSSVDD406 pKa = 3.51GSIAQFEE413 pKa = 4.73TVNTAGNNSLTVDD426 pKa = 4.16TIEE429 pKa = 6.54DD430 pKa = 3.49NTTWNIQSGSTLFVTNTTGSNVQVNMSSDD459 pKa = 3.44SLVNLGTVGATANSNLVVSNTSMSTANQQNLAIATYY495 pKa = 7.21TTSASNPPNAVSVAFSNGAQQVEE518 pKa = 4.61SRR520 pKa = 11.84NGAYY524 pKa = 10.25NYY526 pKa = 10.67NNSLTQQAQPSTVSNGLLQANNDD549 pKa = 3.23TVYY552 pKa = 11.4NVMFSSSRR560 pKa = 11.84GDD562 pKa = 3.27LASDD566 pKa = 3.67VQGMIAGLDD575 pKa = 3.41AAKK578 pKa = 10.3QAGRR582 pKa = 11.84MITDD586 pKa = 3.95DD587 pKa = 3.62LANRR591 pKa = 11.84LTQVHH596 pKa = 6.14LQNLFGHH603 pKa = 6.44GVDD606 pKa = 4.9GAQVWGDD613 pKa = 3.37FLYY616 pKa = 11.17QNGDD620 pKa = 3.27YY621 pKa = 11.03SDD623 pKa = 4.45DD624 pKa = 3.52VDD626 pKa = 4.88YY627 pKa = 11.63KK628 pKa = 11.26DD629 pKa = 3.24ITQGVQGGVDD639 pKa = 3.21WTTHH643 pKa = 5.26LNNGDD648 pKa = 3.99SLTGGIALGWTRR660 pKa = 11.84SRR662 pKa = 11.84DD663 pKa = 3.17RR664 pKa = 11.84STNGGSNNFNDD675 pKa = 3.87SVYY678 pKa = 10.0GNYY681 pKa = 10.18YY682 pKa = 9.9SVYY685 pKa = 10.54GGWQQSLHH693 pKa = 6.54DD694 pKa = 4.68NLWGMFVDD702 pKa = 5.66GSFSYY707 pKa = 11.74GDD709 pKa = 3.37MRR711 pKa = 11.84YY712 pKa = 8.29STSANNVSNATTGMTQALDD731 pKa = 3.75GSSDD735 pKa = 3.4GNLYY739 pKa = 6.87TTQARR744 pKa = 11.84AGVNVVLPGEE754 pKa = 4.47TVIQPYY760 pKa = 8.31ATLGWDD766 pKa = 3.32KK767 pKa = 11.07AQEE770 pKa = 4.56DD771 pKa = 4.83GFSDD775 pKa = 3.55QAITFGDD782 pKa = 3.87SQVSEE787 pKa = 4.3WNTGIGMRR795 pKa = 11.84VTTKK799 pKa = 10.76LADD802 pKa = 3.73LNKK805 pKa = 10.33NVEE808 pKa = 4.32LYY810 pKa = 9.19PWLDD814 pKa = 2.81ARR816 pKa = 11.84YY817 pKa = 7.97QTEE820 pKa = 4.03FSDD823 pKa = 3.66NTDD826 pKa = 2.63IKK828 pKa = 11.07AADD831 pKa = 3.48YY832 pKa = 11.27HH833 pKa = 5.71NTNGHH838 pKa = 6.08NATMGIFGAGINTTIGKK855 pKa = 9.5DD856 pKa = 3.48FSLNTGVYY864 pKa = 10.06FGTGDD869 pKa = 3.35VDD871 pKa = 3.65NDD873 pKa = 3.34ASVQAGVSYY882 pKa = 10.85HH883 pKa = 5.72FF884 pKa = 4.73

Molecular weight: 91.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A248KKU6|A0A248KKU6_9ENTR Peptide transporter OS=Kluyvera genomosp. 3 OX=2774055 GN=CEW81_19245 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.16GRR39 pKa = 11.84SRR41 pKa = 11.84LTVSKK46 pKa = 11.03

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2234

0

2234

514278

26

1407

230.2

25.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.785 ± 0.067

1.142 ± 0.021

5.374 ± 0.043

5.734 ± 0.055

3.835 ± 0.043

7.255 ± 0.052

2.281 ± 0.028

5.8 ± 0.05

4.554 ± 0.049

10.293 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.824 ± 0.028

3.959 ± 0.042

4.134 ± 0.037

4.443 ± 0.042

5.588 ± 0.05

5.992 ± 0.046

5.542 ± 0.04

7.179 ± 0.048

1.401 ± 0.023

2.886 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski