Bacillus phage B4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Bequatrovirus; Bacillus virus B4

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 277 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9PW25|J9PW25_9CAUD Uncharacterized protein OS=Bacillus phage B4 OX=1141133 GN=BCB4_0055 PE=4 SV=1
MM1 pKa = 7.93DD2 pKa = 5.5VEE4 pKa = 4.43QCWDD8 pKa = 3.48CGGLVSSGIIWDD20 pKa = 4.02DD21 pKa = 3.41KK22 pKa = 11.11VICFPCIYY30 pKa = 10.7AEE32 pKa = 4.2DD33 pKa = 3.99EE34 pKa = 3.98RR35 pKa = 11.84RR36 pKa = 11.84EE37 pKa = 3.8QDD39 pKa = 3.17GEE41 pKa = 4.41SNRR44 pKa = 11.84EE45 pKa = 3.78VGTDD49 pKa = 3.21MM50 pKa = 5.94

Molecular weight: 5.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9PUR5|J9PUR5_9CAUD Uncharacterized protein OS=Bacillus phage B4 OX=1141133 GN=BCB4_0058 PE=4 SV=1
MM1 pKa = 6.84TRR3 pKa = 11.84HH4 pKa = 5.4VRR6 pKa = 11.84YY7 pKa = 9.46KK8 pKa = 10.23QLNKK12 pKa = 9.81ATIKK16 pKa = 10.38QATINSIMLRR26 pKa = 11.84TPSIEE31 pKa = 4.23EE32 pKa = 3.47NRR34 pKa = 11.84QVYY37 pKa = 9.8KK38 pKa = 11.25AMGILDD44 pKa = 4.1EE45 pKa = 4.64GFLWFSPRR53 pKa = 11.84RR54 pKa = 11.84WHH56 pKa = 5.51QVKK59 pKa = 7.8MTNLIYY65 pKa = 10.1LTGKK69 pKa = 10.29EE70 pKa = 4.47SFDD73 pKa = 3.36AMKK76 pKa = 10.81ARR78 pKa = 11.84IFQIIDD84 pKa = 2.96VRR86 pKa = 11.84TWKK89 pKa = 10.72

Molecular weight: 10.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

277

0

277

48831

43

2019

176.3

20.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.963 ± 0.204

0.817 ± 0.063

6.387 ± 0.119

8.491 ± 0.311

3.977 ± 0.104

6.498 ± 0.216

1.837 ± 0.106

6.578 ± 0.121

8.153 ± 0.195

8.101 ± 0.177

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.83 ± 0.089

5.337 ± 0.151

2.912 ± 0.15

3.32 ± 0.153

4.29 ± 0.124

5.37 ± 0.141

6.242 ± 0.237

6.897 ± 0.167

1.218 ± 0.06

4.782 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski