Fictibacillus enclensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Fictibacillus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4409 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0V8JCV5|A0A0V8JCV5_9BACI Molybdenum cofactor biosynthesis protein MoaE OS=Fictibacillus enclensis OX=1017270 GN=AS030_04675 PE=4 SV=1
MM1 pKa = 7.77EE2 pKa = 4.41YY3 pKa = 10.87SLYY6 pKa = 10.74KK7 pKa = 10.06KK8 pKa = 10.54DD9 pKa = 3.63GAFPCDD15 pKa = 3.31VTIDD19 pKa = 3.65VDD21 pKa = 3.92NNIYY25 pKa = 7.96TVRR28 pKa = 11.84DD29 pKa = 3.44SDD31 pKa = 3.67TTGQIFQSAPEE42 pKa = 3.86VASWIKK48 pKa = 10.24QNWAPDD54 pKa = 3.7QFEE57 pKa = 4.81KK58 pKa = 10.88PDD60 pKa = 3.81DD61 pKa = 4.28YY62 pKa = 11.85YY63 pKa = 11.79DD64 pKa = 3.75LVNTLEE70 pKa = 4.63SSLQEE75 pKa = 4.31DD76 pKa = 4.04EE77 pKa = 5.52LGII80 pKa = 4.31

Molecular weight: 9.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0V8J896|A0A0V8J896_9BACI Protein translocase subunit SecA OS=Fictibacillus enclensis OX=1017270 GN=secA PE=3 SV=1
MM1 pKa = 7.43LRR3 pKa = 11.84FIGQFVIILATLAAGNALSSFFHH26 pKa = 6.58LPVPGSILGMFLLFLGLLTGVVKK49 pKa = 10.37LRR51 pKa = 11.84WVEE54 pKa = 4.37GVANSHH60 pKa = 5.99LRR62 pKa = 11.84HH63 pKa = 5.31MTLLFIPPVVGLFLNWGAVHH83 pKa = 5.76IQLWKK88 pKa = 10.13VLLVLSLSSLCILLGTGYY106 pKa = 10.47SVEE109 pKa = 4.37LYY111 pKa = 9.55EE112 pKa = 4.45KK113 pKa = 10.34RR114 pKa = 11.84KK115 pKa = 9.54RR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84NEE120 pKa = 3.23

Molecular weight: 13.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4409

0

4409

1274383

26

1520

289.0

32.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.415 ± 0.038

0.752 ± 0.012

4.957 ± 0.031

7.256 ± 0.048

4.418 ± 0.031

7.314 ± 0.034

2.205 ± 0.019

7.135 ± 0.037

6.874 ± 0.039

9.846 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.841 ± 0.018

4.129 ± 0.023

3.866 ± 0.022

3.73 ± 0.029

4.205 ± 0.028

6.15 ± 0.026

5.291 ± 0.023

7.116 ± 0.029

1.089 ± 0.016

3.409 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski