African eggplant yellowing virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; unclassified Polerovirus

Average proteome isoelectric point is 8.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S6PCS2|A0A1S6PCS2_9LUTE Readthrough protein OS=African eggplant yellowing virus OX=1963256 PE=3 SV=1
MM1 pKa = 7.37VKK3 pKa = 10.03ILLALFAFFSLFSLGTPSHH22 pKa = 6.8LGLPLDD28 pKa = 3.76GTLFRR33 pKa = 11.84DD34 pKa = 3.67SGNEE38 pKa = 4.02LIASPSTAEE47 pKa = 3.96LRR49 pKa = 11.84HH50 pKa = 5.85FPSINLSVLRR60 pKa = 11.84PMRR63 pKa = 11.84SPGRR67 pKa = 11.84EE68 pKa = 3.83TLDD71 pKa = 3.51SKK73 pKa = 11.74DD74 pKa = 3.49LTFSDD79 pKa = 4.93AYY81 pKa = 10.66RR82 pKa = 11.84LMWEE86 pKa = 3.92VSLRR90 pKa = 11.84DD91 pKa = 3.8TKK93 pKa = 10.9HH94 pKa = 6.26LSLEE98 pKa = 4.02ATRR101 pKa = 11.84ILQNSFASGRR111 pKa = 11.84ATLTNCLEE119 pKa = 4.28KK120 pKa = 10.96ALGALLWMIVWIWSSVIWAFGYY142 pKa = 6.2WTFYY146 pKa = 10.42MVTTFTMPVVCLALLFAFTKK166 pKa = 10.42FMVLAAEE173 pKa = 4.46KK174 pKa = 9.66MLAGWPVYY182 pKa = 10.06LGKK185 pKa = 10.82LFLRR189 pKa = 11.84LLKK192 pKa = 9.61TASTALFSKK201 pKa = 10.41RR202 pKa = 11.84NYY204 pKa = 9.99VPEE207 pKa = 4.2KK208 pKa = 9.76SVKK211 pKa = 10.29GFISVKK217 pKa = 9.9IPQSPPRR224 pKa = 11.84GSVLLIQHH232 pKa = 7.41DD233 pKa = 4.53DD234 pKa = 3.8GSHH237 pKa = 6.41AGYY240 pKa = 10.6ASCVKK245 pKa = 10.66LYY247 pKa = 10.92DD248 pKa = 3.51GTLALMTCEE257 pKa = 3.98HH258 pKa = 6.57VATGVEE264 pKa = 4.14GGRR267 pKa = 11.84VVSAKK272 pKa = 9.14TRR274 pKa = 11.84NKK276 pKa = 10.03IPLNLFTPLLISQKK290 pKa = 10.96GDD292 pKa = 3.14FALLSGPPNWEE303 pKa = 3.87SLLGCKK309 pKa = 9.97GAMFVPASQLAKK321 pKa = 10.42SKK323 pKa = 9.38MRR325 pKa = 11.84FYY327 pKa = 10.78FVEE330 pKa = 4.09NEE332 pKa = 3.47EE333 pKa = 3.88WMADD337 pKa = 3.17HH338 pKa = 6.89GEE340 pKa = 4.3VVSARR345 pKa = 11.84DD346 pKa = 3.02HH347 pKa = 6.07WFATTLCNSEE357 pKa = 4.56PGFSGTPIFNGKK369 pKa = 7.73TIIGVHH375 pKa = 6.16AGSEE379 pKa = 4.18VEE381 pKa = 4.25EE382 pKa = 4.34NSNLMSVIPPVPGLTTPQYY401 pKa = 10.89VYY403 pKa = 9.56EE404 pKa = 4.34TTAPQGRR411 pKa = 11.84LFIDD415 pKa = 3.48EE416 pKa = 4.66DD417 pKa = 4.34VSVLLKK423 pKa = 10.51SVKK426 pKa = 9.31TRR428 pKa = 11.84IPDD431 pKa = 3.04IAKK434 pKa = 9.25YY435 pKa = 10.73VSVSGKK441 pKa = 8.92NWADD445 pKa = 3.38YY446 pKa = 10.94RR447 pKa = 11.84SDD449 pKa = 4.31DD450 pKa = 4.57DD451 pKa = 5.76DD452 pKa = 6.11DD453 pKa = 4.89FFEE456 pKa = 5.12SSVTFEE462 pKa = 5.1GEE464 pKa = 4.14TKK466 pKa = 9.76PAPIAEE472 pKa = 4.45PVTPPIQFGTVPEE485 pKa = 4.32APVGKK490 pKa = 8.45QQWVPRR496 pKa = 11.84AQPSSGNRR504 pKa = 11.84EE505 pKa = 3.98RR506 pKa = 11.84QSCLPKK512 pKa = 9.71QQHH515 pKa = 6.52PSSEE519 pKa = 4.2AKK521 pKa = 9.93AHH523 pKa = 6.12SGGKK527 pKa = 9.87AKK529 pKa = 10.82AKK531 pKa = 10.23GPRR534 pKa = 11.84NQHH537 pKa = 5.06PAKK540 pKa = 9.97AARR543 pKa = 11.84IARR546 pKa = 11.84KK547 pKa = 9.02RR548 pKa = 11.84AQAARR553 pKa = 11.84AAEE556 pKa = 3.92EE557 pKa = 3.73RR558 pKa = 11.84AGNVRR563 pKa = 11.84KK564 pKa = 9.59AHH566 pKa = 6.2GEE568 pKa = 3.75NGGEE572 pKa = 4.04DD573 pKa = 3.01RR574 pKa = 11.84PIRR577 pKa = 11.84HH578 pKa = 6.19RR579 pKa = 11.84KK580 pKa = 8.43EE581 pKa = 4.03SGASSCRR588 pKa = 11.84EE589 pKa = 3.79GNEE592 pKa = 3.68EE593 pKa = 4.24AKK595 pKa = 10.5RR596 pKa = 11.84LSAQEE601 pKa = 3.37IAEE604 pKa = 4.02NFEE607 pKa = 4.02AHH609 pKa = 6.98FGGLYY614 pKa = 8.62EE615 pKa = 4.45WEE617 pKa = 4.49VSTSPQEE624 pKa = 3.66IPGFEE629 pKa = 4.03RR630 pKa = 11.84CGCLPRR636 pKa = 11.84YY637 pKa = 7.96YY638 pKa = 10.28FPKK641 pKa = 10.14QIQGSSWGAQLIEE654 pKa = 4.07EE655 pKa = 4.38HH656 pKa = 7.15PEE658 pKa = 3.94LGEE661 pKa = 4.08KK662 pKa = 10.03VAGFGWPLVGPRR674 pKa = 11.84AEE676 pKa = 4.33VTSLTLQAEE685 pKa = 4.3RR686 pKa = 11.84WLQRR690 pKa = 11.84AQSAKK695 pKa = 10.15IPSTEE700 pKa = 3.64DD701 pKa = 2.93RR702 pKa = 11.84EE703 pKa = 4.26RR704 pKa = 11.84VINKK708 pKa = 8.39TVDD711 pKa = 2.72AYY713 pKa = 10.34KK714 pKa = 10.37DD715 pKa = 3.72VKK717 pKa = 10.49TMGPTATRR725 pKa = 11.84GNKK728 pKa = 10.04LEE730 pKa = 3.9WSQFLEE736 pKa = 4.24DD737 pKa = 5.16FKK739 pKa = 11.03TAVNSLEE746 pKa = 3.99LDD748 pKa = 3.3AGIGVPYY755 pKa = 10.1ISYY758 pKa = 10.38GRR760 pKa = 11.84PTHH763 pKa = 6.43RR764 pKa = 11.84GWVEE768 pKa = 3.89DD769 pKa = 3.99PKK771 pKa = 11.09LLPVLARR778 pKa = 11.84LTFSRR783 pKa = 11.84LQKK786 pKa = 9.58MLEE789 pKa = 4.05VEE791 pKa = 4.35ADD793 pKa = 3.67LLTAEE798 pKa = 4.38QLVQEE803 pKa = 4.85GLCDD807 pKa = 4.87PIRR810 pKa = 11.84VFVKK814 pKa = 10.54RR815 pKa = 11.84EE816 pKa = 3.54PHH818 pKa = 5.83KK819 pKa = 10.47QSKK822 pKa = 10.11LDD824 pKa = 3.38EE825 pKa = 4.27GRR827 pKa = 11.84YY828 pKa = 8.96RR829 pKa = 11.84LIMSVSLVDD838 pKa = 3.45QLVARR843 pKa = 11.84VLFQNQNKK851 pKa = 9.66RR852 pKa = 11.84EE853 pKa = 3.89IALWRR858 pKa = 11.84CNPSKK863 pKa = 10.66PGFGLSTDD871 pKa = 3.63EE872 pKa = 3.99QVLEE876 pKa = 4.47FVEE879 pKa = 4.34TLAAQVEE886 pKa = 4.78VTPEE890 pKa = 3.77DD891 pKa = 5.07LVASWEE897 pKa = 4.34KK898 pKa = 10.96YY899 pKa = 9.98LVPTDD904 pKa = 3.67CSGFDD909 pKa = 3.21WSVAEE914 pKa = 4.57WMLHH918 pKa = 5.89DD919 pKa = 5.83DD920 pKa = 3.43MVVRR924 pKa = 11.84NKK926 pKa = 9.42LTLDD930 pKa = 3.76LNSTTEE936 pKa = 4.06KK937 pKa = 10.64LRR939 pKa = 11.84KK940 pKa = 7.13TWLRR944 pKa = 11.84CISNSVLCLSDD955 pKa = 3.31GTLLAQRR962 pKa = 11.84VPGVQKK968 pKa = 10.45SGSYY972 pKa = 8.25NTSSSNSRR980 pKa = 11.84IRR982 pKa = 11.84VMAAYY987 pKa = 9.63HH988 pKa = 6.86AGASWAIAMGDD999 pKa = 3.51DD1000 pKa = 3.67ALEE1003 pKa = 4.26SVNTNLEE1010 pKa = 4.32VYY1012 pKa = 10.11KK1013 pKa = 10.98SLGFKK1018 pKa = 10.72VEE1020 pKa = 3.93VSGQLEE1026 pKa = 4.4FCSHH1030 pKa = 6.9IFRR1033 pKa = 11.84APDD1036 pKa = 2.91LATPVNEE1043 pKa = 3.98NKK1045 pKa = 9.38MLYY1048 pKa = 10.08KK1049 pKa = 10.68LIYY1052 pKa = 9.23GYY1054 pKa = 10.97NVGCGNLEE1062 pKa = 4.03VVSNYY1067 pKa = 9.16IAACASVLNEE1077 pKa = 4.41LRR1079 pKa = 11.84HH1080 pKa = 6.43DD1081 pKa = 4.37PDD1083 pKa = 3.94SVALLYY1089 pKa = 10.28QWLVDD1094 pKa = 3.49PVLPQKK1100 pKa = 10.43II1101 pKa = 3.75

Molecular weight: 122.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S6PCU6|A0A1S6PCU6_9LUTE p3 OS=African eggplant yellowing virus OX=1963256 PE=4 SV=1
MM1 pKa = 6.8NTGGRR6 pKa = 11.84KK7 pKa = 8.27NVNYY11 pKa = 9.86RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84QRR17 pKa = 11.84RR18 pKa = 11.84TVRR21 pKa = 11.84PVQQMVVAVPSGQPRR36 pKa = 11.84GRR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84NRR44 pKa = 11.84NRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84GVRR51 pKa = 11.84GGPRR55 pKa = 11.84SGGSSNSEE63 pKa = 3.35TFVFNKK69 pKa = 10.46DD70 pKa = 3.59SIKK73 pKa = 10.99DD74 pKa = 3.68NSAGTIKK81 pKa = 10.65FGPNLSEE88 pKa = 4.82SIALSGGVLKK98 pKa = 10.54AYY100 pKa = 10.22HH101 pKa = 6.68EE102 pKa = 4.5YY103 pKa = 10.51KK104 pKa = 9.32ITMVNIRR111 pKa = 11.84FVSEE115 pKa = 4.1SSSTAEE121 pKa = 3.6GSIAYY126 pKa = 9.39EE127 pKa = 4.08LDD129 pKa = 3.29PHH131 pKa = 7.03CKK133 pKa = 10.0LKK135 pKa = 10.89SLQSTLRR142 pKa = 11.84KK143 pKa = 9.77FPVTKK148 pKa = 10.49GGSTTFRR155 pKa = 11.84AAQINGVEE163 pKa = 4.07WHH165 pKa = 7.01DD166 pKa = 3.65TADD169 pKa = 3.53DD170 pKa = 3.93QFFLHH175 pKa = 6.22YY176 pKa = 10.23AGNGTSGKK184 pKa = 8.04TAGFLQIRR192 pKa = 11.84FTVQLHH198 pKa = 5.06NPKK201 pKa = 10.54

Molecular weight: 22.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3072

154

1101

512.0

56.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.552 ± 0.501

1.335 ± 0.349

4.46 ± 0.471

5.957 ± 0.496

4.264 ± 0.243

7.389 ± 0.623

2.116 ± 0.179

4.199 ± 0.394

5.664 ± 0.608

8.984 ± 1.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.758 ± 0.261

3.809 ± 0.447

6.283 ± 0.447

3.516 ± 0.199

6.836 ± 1.097

8.952 ± 0.639

5.762 ± 0.41

6.771 ± 0.454

1.823 ± 0.238

2.539 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski