Beet necrotic yellow vein virus (isolate Japan/S) (BNYVV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Hepelivirales; Benyviridae; Benyvirus; Beet necrotic yellow vein virus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q65674|TGB1_BNYVS Movement protein TGB1 OS=Beet necrotic yellow vein virus (isolate Japan/S) OX=652670 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.92ADD3 pKa = 4.27GEE5 pKa = 4.3ICRR8 pKa = 11.84CQVTDD13 pKa = 3.84PPLIRR18 pKa = 11.84HH19 pKa = 6.19EE20 pKa = 6.0DD21 pKa = 3.69YY22 pKa = 11.28DD23 pKa = 3.84CTARR27 pKa = 11.84MVQKK31 pKa = 10.31RR32 pKa = 11.84IEE34 pKa = 4.12IGPLGVLLNLNMLFHH49 pKa = 7.04MSRR52 pKa = 11.84VRR54 pKa = 11.84HH55 pKa = 5.81IDD57 pKa = 3.14VYY59 pKa = 10.92PYY61 pKa = 10.66LNNIMSISVSLDD73 pKa = 3.18VPVSSGVGVGRR84 pKa = 11.84VRR86 pKa = 11.84VLIFTTSRR94 pKa = 11.84EE95 pKa = 3.95RR96 pKa = 11.84VGIFHH101 pKa = 7.32GWQVVPGCFLNAPCYY116 pKa = 10.55SGVDD120 pKa = 3.59VLSDD124 pKa = 3.7EE125 pKa = 4.59LCEE128 pKa = 4.4ANITNTSVSSVAMFNGSYY146 pKa = 9.99RR147 pKa = 11.84PEE149 pKa = 4.28DD150 pKa = 3.47VWILLLTSSTCYY162 pKa = 10.05GYY164 pKa = 10.96HH165 pKa = 6.77DD166 pKa = 3.93VVVDD170 pKa = 4.21IEE172 pKa = 4.37QCTLPSNIDD181 pKa = 3.29GCVCCSGVCYY191 pKa = 10.57FNDD194 pKa = 3.17NHH196 pKa = 6.23CFCGRR201 pKa = 11.84RR202 pKa = 11.84DD203 pKa = 3.85SNPFNPPCFQFIKK216 pKa = 10.55DD217 pKa = 3.83CNEE220 pKa = 3.88LYY222 pKa = 9.07GTNEE226 pKa = 4.07TKK228 pKa = 10.59QFICDD233 pKa = 3.88LVGDD237 pKa = 4.77DD238 pKa = 5.62NLDD241 pKa = 3.66SVNTLTKK248 pKa = 9.77EE249 pKa = 3.74GWRR252 pKa = 11.84RR253 pKa = 11.84FCDD256 pKa = 3.56VLWNTTYY263 pKa = 11.61GDD265 pKa = 3.61VEE267 pKa = 4.45SRR269 pKa = 11.84TFARR273 pKa = 11.84FLWFVFYY280 pKa = 10.94HH281 pKa = 6.88DD282 pKa = 3.8

Molecular weight: 31.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q65676|TGB3_BNYVS Movement protein TGB3 OS=Beet necrotic yellow vein virus (isolate Japan/S) OX=652670 PE=3 SV=1
MM1 pKa = 7.3SRR3 pKa = 11.84EE4 pKa = 3.35ITARR8 pKa = 11.84PNKK11 pKa = 9.69NVPIVVGVCVVAFFVLLAFMQQKK34 pKa = 10.37HH35 pKa = 5.0KK36 pKa = 8.76THH38 pKa = 6.44SGGDD42 pKa = 3.43YY43 pKa = 10.74GVPTFSNGGKK53 pKa = 9.9YY54 pKa = 9.73RR55 pKa = 11.84DD56 pKa = 3.9GTRR59 pKa = 11.84SADD62 pKa = 3.47FNSNNHH68 pKa = 5.99RR69 pKa = 11.84AYY71 pKa = 10.45GCGGSGGSVSSRR83 pKa = 11.84VGQQLVVLAIVSVLIVSLLQRR104 pKa = 11.84LRR106 pKa = 11.84SPPEE110 pKa = 4.12HH111 pKa = 6.74ICNGACGG118 pKa = 3.35

Molecular weight: 12.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

1

10

4479

118

2109

447.9

49.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.988 ± 1.093

2.3 ± 0.757

6.586 ± 0.567

5.046 ± 0.624

4.555 ± 0.522

6.698 ± 0.774

2.367 ± 0.291

4.197 ± 0.32

5.09 ± 0.862

9.243 ± 0.486

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.88 ± 0.249

4.867 ± 0.385

3.862 ± 0.245

2.612 ± 0.288

5.604 ± 0.423

8.127 ± 0.56

5.023 ± 0.624

9.221 ± 0.66

1.496 ± 0.158

3.215 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski