Bacillus virus Wbeta

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Wbetavirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2LIH5|Q2LIH5_9CAUD Putative major tail protein OS=Bacillus virus Wbeta OX=359961 GN=wp10 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 9.15LTLMINKK9 pKa = 8.29EE10 pKa = 4.02KK11 pKa = 9.69QTFNMPEE18 pKa = 4.57FIPARR23 pKa = 11.84LIRR26 pKa = 11.84QAPEE30 pKa = 3.29LAEE33 pKa = 4.24IPNNPGPEE41 pKa = 4.29DD42 pKa = 3.77MDD44 pKa = 3.93KK45 pKa = 10.42MVQFVVKK52 pKa = 10.59VYY54 pKa = 10.72DD55 pKa = 3.7GQFTLDD61 pKa = 3.74QYY63 pKa = 11.42WDD65 pKa = 3.62GVDD68 pKa = 3.17ARR70 pKa = 11.84KK71 pKa = 9.13FLSTTSDD78 pKa = 3.58VINAIINEE86 pKa = 4.3TVEE89 pKa = 4.01AAGGSTEE96 pKa = 4.23SGEE99 pKa = 4.31EE100 pKa = 3.99EE101 pKa = 4.29NPNAA105 pKa = 4.92

Molecular weight: 11.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2LIG5|Q2LIG5_9CAUD Conserved phage protein OS=Bacillus virus Wbeta OX=359961 GN=wp20 PE=4 SV=1
MM1 pKa = 7.18TLAGEE6 pKa = 5.05AIIIWTATGLSVVAMKK22 pKa = 10.43AAEE25 pKa = 4.13KK26 pKa = 9.39MGKK29 pKa = 9.06SVPHH33 pKa = 6.06WLPRR37 pKa = 11.84VTLYY41 pKa = 7.6TTLTGSFLYY50 pKa = 10.53LLRR53 pKa = 11.84YY54 pKa = 9.77VLVLFLL60 pKa = 5.08

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12243

56

1331

231.0

26.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.306 ± 0.496

0.858 ± 0.162

5.66 ± 0.25

8.437 ± 0.371

3.823 ± 0.227

6.453 ± 0.732

1.576 ± 0.194

7.122 ± 0.299

9.222 ± 0.423

8.339 ± 0.388

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.704 ± 0.178

5.334 ± 0.242

3.022 ± 0.537

4.296 ± 0.294

4.648 ± 0.245

5.775 ± 0.275

5.726 ± 0.428

6.077 ± 0.185

1.045 ± 0.142

3.578 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski