Vibrio atlanticus (strain LGP32) (Vibrio splendidus (strain Mel32))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio; Vibrio atlanticus

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4420 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7VKF1|B7VKF1_VIBA3 Peptidoglycan glycosyltransferase MrdB OS=Vibrio atlanticus (strain LGP32) OX=575788 GN=mrdB PE=3 SV=1
MM1 pKa = 7.7KK2 pKa = 10.13YY3 pKa = 10.75SPILAVALSALFIVGCNDD21 pKa = 3.27DD22 pKa = 4.83DD23 pKa = 4.76QPTTQLQAVHH33 pKa = 7.15ASPDD37 pKa = 3.37APLANVLVNSQARR50 pKa = 11.84WTGVDD55 pKa = 3.35YY56 pKa = 11.18AQASGYY62 pKa = 9.38TSVNQGQTTLQVDD75 pKa = 3.78VQLPGDD81 pKa = 4.04GVATVIPPSQFDD93 pKa = 3.57LSGDD97 pKa = 3.33LDD99 pKa = 3.96YY100 pKa = 11.53TVMVVGDD107 pKa = 4.24ADD109 pKa = 3.74GSNNPVEE116 pKa = 4.38ALVVTRR122 pKa = 11.84PAAGTATSSSLDD134 pKa = 3.51VQVVHH139 pKa = 7.02AATGVGDD146 pKa = 3.6VNLYY150 pKa = 8.08VTAPNDD156 pKa = 3.66PLGAPIGTLGYY167 pKa = 10.67KK168 pKa = 10.61GFTDD172 pKa = 3.8VLNIPAGQYY181 pKa = 9.21RR182 pKa = 11.84VRR184 pKa = 11.84LEE186 pKa = 4.29TVSGSAIAFDD196 pKa = 3.86SGEE199 pKa = 3.91ITLPAGSEE207 pKa = 3.96LTIAAVPRR215 pKa = 11.84ADD217 pKa = 3.58SSSTSPVKK225 pKa = 10.77LMVMDD230 pKa = 5.36GKK232 pKa = 10.73SSSIIYY238 pKa = 10.65DD239 pKa = 3.31MAEE242 pKa = 3.9SAEE245 pKa = 3.99VRR247 pKa = 11.84VGHH250 pKa = 6.64LVDD253 pKa = 4.71GAPSVDD259 pKa = 3.6VYY261 pKa = 11.85VNSAKK266 pKa = 10.23FAPLDD271 pKa = 3.28ALMFKK276 pKa = 9.58EE277 pKa = 4.22VRR279 pKa = 11.84GFLDD283 pKa = 4.14LAAGSYY289 pKa = 10.78DD290 pKa = 2.95IDD292 pKa = 3.57IYY294 pKa = 10.4EE295 pKa = 4.43TATTSPTFIDD305 pKa = 3.39VDD307 pKa = 3.85GLAVFAGMDD316 pKa = 3.37YY317 pKa = 10.84SIYY320 pKa = 10.93AVGTVSPLNLEE331 pKa = 4.1ALVVPEE337 pKa = 4.13NRR339 pKa = 11.84RR340 pKa = 11.84SVATSAVLNITHH352 pKa = 7.49AAANPIAASVDD363 pKa = 2.94IYY365 pKa = 10.54LTEE368 pKa = 4.0NVGISGSTPALSNVKK383 pKa = 10.29FKK385 pKa = 11.38DD386 pKa = 3.65FANGIYY392 pKa = 9.76VAAGTYY398 pKa = 9.95YY399 pKa = 9.56VTITVAGDD407 pKa = 3.48PSTVAIDD414 pKa = 3.61SAPATLVNGVVYY426 pKa = 9.48QVVAIDD432 pKa = 4.17DD433 pKa = 3.93SMGTGFNLIVSEE445 pKa = 4.34TTDD448 pKa = 2.85

Molecular weight: 46.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7VGL3|B7VGL3_VIBA3 3-ketoacyl-CoA thiolase OS=Vibrio atlanticus (strain LGP32) OX=575788 GN=fadA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.5RR3 pKa = 11.84TFQPTVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 7.65RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44NGRR28 pKa = 11.84ATINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSKK44 pKa = 10.84

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4420

0

4420

1422917

21

5372

321.9

35.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.296 ± 0.044

0.997 ± 0.013

5.604 ± 0.041

6.506 ± 0.036

4.192 ± 0.026

6.739 ± 0.038

2.178 ± 0.021

6.373 ± 0.029

5.423 ± 0.036

10.225 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.701 ± 0.02

4.342 ± 0.027

3.801 ± 0.021

4.474 ± 0.032

4.25 ± 0.03

7.026 ± 0.035

5.502 ± 0.037

7.136 ± 0.034

1.224 ± 0.015

3.01 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski