Hubei narna-like virus 25

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KI82|A0A1L3KI82_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 25 OX=1922956 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 7.96MTKK5 pKa = 10.37FNMFKK10 pKa = 9.54VTLKK14 pKa = 10.24VVTWMFQGFNLKK26 pKa = 9.7MSGRR30 pKa = 11.84MSDD33 pKa = 3.87YY34 pKa = 11.26LDD36 pKa = 5.73LILSLDD42 pKa = 3.35RR43 pKa = 11.84LLYY46 pKa = 10.6NRR48 pKa = 11.84GALEE52 pKa = 3.71TLKK55 pKa = 10.69YY56 pKa = 10.47IKK58 pKa = 9.74GTSTAYY64 pKa = 10.3LAYY67 pKa = 10.7LSGKK71 pKa = 7.64PMRR74 pKa = 11.84VKK76 pKa = 10.62GVSTTKK82 pKa = 10.76DD83 pKa = 3.82GIPVILGDD91 pKa = 4.19FVPKK95 pKa = 9.81IRR97 pKa = 11.84KK98 pKa = 9.3GPTPAMLQLINTILFCTSALKK119 pKa = 10.77LGSTPDD125 pKa = 4.1FSPMINPPKK134 pKa = 10.11SDD136 pKa = 4.77PINMGEE142 pKa = 4.28FVDD145 pKa = 5.58DD146 pKa = 4.63FWRR149 pKa = 11.84DD150 pKa = 3.31MGYY153 pKa = 10.08HH154 pKa = 5.52RR155 pKa = 11.84QARR158 pKa = 11.84TNPKK162 pKa = 9.63SLNFRR167 pKa = 11.84KK168 pKa = 9.29FHH170 pKa = 6.31LTTKK174 pKa = 10.19SGPNGTANALWTSLNDD190 pKa = 3.66LKK192 pKa = 11.24ALSEE196 pKa = 4.1QQLTDD201 pKa = 2.87IGIIGGEE208 pKa = 4.12TLRR211 pKa = 11.84SKK213 pKa = 10.51MSTLKK218 pKa = 10.74EE219 pKa = 4.12GLDD222 pKa = 3.66KK223 pKa = 11.37VPEE226 pKa = 4.05LSYY229 pKa = 11.39FMDD232 pKa = 3.98TSAGVYY238 pKa = 6.95TSRR241 pKa = 11.84MTSFPDD247 pKa = 3.06KK248 pKa = 9.45EE249 pKa = 4.23LKK251 pKa = 10.43VRR253 pKa = 11.84VIAVGDD259 pKa = 3.97YY260 pKa = 10.13FSQAALKK267 pKa = 9.53PLHH270 pKa = 7.32DD271 pKa = 3.79YY272 pKa = 11.0LFRR275 pKa = 11.84VLKK278 pKa = 10.23KK279 pKa = 10.22IPQDD283 pKa = 3.34CTFNQGGFWDD293 pKa = 4.38KK294 pKa = 11.11IKK296 pKa = 10.8DD297 pKa = 3.55QEE299 pKa = 4.54YY300 pKa = 9.82YY301 pKa = 11.37ASIDD305 pKa = 3.31LTAATDD311 pKa = 3.6SFPISTMSQVLLGSLPKK328 pKa = 10.33SYY330 pKa = 11.12VDD332 pKa = 2.73AWHH335 pKa = 7.01RR336 pKa = 11.84LMVGTPFIYY345 pKa = 10.12EE346 pKa = 4.12GQEE349 pKa = 3.34MNYY352 pKa = 10.83AVGNPMGFYY361 pKa = 10.68SSWASFAVAHH371 pKa = 6.5HH372 pKa = 5.41YY373 pKa = 9.52VVYY376 pKa = 9.96YY377 pKa = 9.81CCKK380 pKa = 10.41KK381 pKa = 10.84LGIPWKK387 pKa = 7.7TLKK390 pKa = 10.82YY391 pKa = 10.26CLLGDD396 pKa = 5.19DD397 pKa = 5.68IVMCDD402 pKa = 3.57PKK404 pKa = 11.13VAALYY409 pKa = 10.64KK410 pKa = 9.44EE411 pKa = 4.68TISGLGVEE419 pKa = 5.03FSEE422 pKa = 4.83EE423 pKa = 3.79KK424 pKa = 10.4TITSPHH430 pKa = 5.77LVEE433 pKa = 4.07FAKK436 pKa = 10.7RR437 pKa = 11.84LIYY440 pKa = 10.42KK441 pKa = 7.7GTEE444 pKa = 3.65MSPFPISALGEE455 pKa = 4.19LANKK459 pKa = 10.31YY460 pKa = 10.41YY461 pKa = 11.2LLTDD465 pKa = 4.19LFLEE469 pKa = 4.73LEE471 pKa = 4.37SKK473 pKa = 10.6GWVSLGGAPSAVEE486 pKa = 4.16SFHH489 pKa = 7.83KK490 pKa = 10.53IVFNWSTVRR499 pKa = 11.84ARR501 pKa = 11.84EE502 pKa = 4.04IEE504 pKa = 4.49VKK506 pKa = 10.73SSVCEE511 pKa = 3.75RR512 pKa = 11.84VMKK515 pKa = 9.47MARR518 pKa = 11.84GAEE521 pKa = 4.09DD522 pKa = 3.85AGTLLTEE529 pKa = 4.69CFSKK533 pKa = 10.77LGYY536 pKa = 9.88PFVLSSFVASNVLEE550 pKa = 4.09NMVVEE555 pKa = 4.94NFANSNPASHH565 pKa = 6.63NPEE568 pKa = 3.67SDD570 pKa = 3.39KK571 pKa = 11.04KK572 pKa = 11.03SKK574 pKa = 10.57SPCVGLGYY582 pKa = 10.25LAEE585 pKa = 4.65NLTCLLTGLDD595 pKa = 4.37EE596 pKa = 4.67EE597 pKa = 4.91SCSLGFDD604 pKa = 3.51LMMALPHH611 pKa = 6.3LNAYY615 pKa = 9.56GQMSEE620 pKa = 5.0LYY622 pKa = 11.09VNINKK627 pKa = 9.84KK628 pKa = 10.07ARR630 pKa = 11.84QISTTNGAEE639 pKa = 3.83WPLLFKK645 pKa = 9.71TMCIPWDD652 pKa = 3.53DD653 pKa = 4.76TIFTMSSSHH662 pKa = 7.14LIAKK666 pKa = 9.41GSSSVVKK673 pKa = 10.39DD674 pKa = 3.62LQNSAEE680 pKa = 4.43LLSFYY685 pKa = 10.49PPEE688 pKa = 3.96EE689 pKa = 4.18LLRR692 pKa = 11.84EE693 pKa = 4.31DD694 pKa = 4.05PNFLKK699 pKa = 10.93SLL701 pKa = 3.77

Molecular weight: 78.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KI82|A0A1L3KI82_9VIRU RNA-dependent RNA polymerase OS=Hubei narna-like virus 25 OX=1922956 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 7.96MTKK5 pKa = 10.37FNMFKK10 pKa = 9.54VTLKK14 pKa = 10.24VVTWMFQGFNLKK26 pKa = 9.7MSGRR30 pKa = 11.84MSDD33 pKa = 3.87YY34 pKa = 11.26LDD36 pKa = 5.73LILSLDD42 pKa = 3.35RR43 pKa = 11.84LLYY46 pKa = 10.6NRR48 pKa = 11.84GALEE52 pKa = 3.71TLKK55 pKa = 10.69YY56 pKa = 10.47IKK58 pKa = 9.74GTSTAYY64 pKa = 10.3LAYY67 pKa = 10.7LSGKK71 pKa = 7.64PMRR74 pKa = 11.84VKK76 pKa = 10.62GVSTTKK82 pKa = 10.76DD83 pKa = 3.82GIPVILGDD91 pKa = 4.19FVPKK95 pKa = 9.81IRR97 pKa = 11.84KK98 pKa = 9.3GPTPAMLQLINTILFCTSALKK119 pKa = 10.77LGSTPDD125 pKa = 4.1FSPMINPPKK134 pKa = 10.11SDD136 pKa = 4.77PINMGEE142 pKa = 4.28FVDD145 pKa = 5.58DD146 pKa = 4.63FWRR149 pKa = 11.84DD150 pKa = 3.31MGYY153 pKa = 10.08HH154 pKa = 5.52RR155 pKa = 11.84QARR158 pKa = 11.84TNPKK162 pKa = 9.63SLNFRR167 pKa = 11.84KK168 pKa = 9.29FHH170 pKa = 6.31LTTKK174 pKa = 10.19SGPNGTANALWTSLNDD190 pKa = 3.66LKK192 pKa = 11.24ALSEE196 pKa = 4.1QQLTDD201 pKa = 2.87IGIIGGEE208 pKa = 4.12TLRR211 pKa = 11.84SKK213 pKa = 10.51MSTLKK218 pKa = 10.74EE219 pKa = 4.12GLDD222 pKa = 3.66KK223 pKa = 11.37VPEE226 pKa = 4.05LSYY229 pKa = 11.39FMDD232 pKa = 3.98TSAGVYY238 pKa = 6.95TSRR241 pKa = 11.84MTSFPDD247 pKa = 3.06KK248 pKa = 9.45EE249 pKa = 4.23LKK251 pKa = 10.43VRR253 pKa = 11.84VIAVGDD259 pKa = 3.97YY260 pKa = 10.13FSQAALKK267 pKa = 9.53PLHH270 pKa = 7.32DD271 pKa = 3.79YY272 pKa = 11.0LFRR275 pKa = 11.84VLKK278 pKa = 10.23KK279 pKa = 10.22IPQDD283 pKa = 3.34CTFNQGGFWDD293 pKa = 4.38KK294 pKa = 11.11IKK296 pKa = 10.8DD297 pKa = 3.55QEE299 pKa = 4.54YY300 pKa = 9.82YY301 pKa = 11.37ASIDD305 pKa = 3.31LTAATDD311 pKa = 3.6SFPISTMSQVLLGSLPKK328 pKa = 10.33SYY330 pKa = 11.12VDD332 pKa = 2.73AWHH335 pKa = 7.01RR336 pKa = 11.84LMVGTPFIYY345 pKa = 10.12EE346 pKa = 4.12GQEE349 pKa = 3.34MNYY352 pKa = 10.83AVGNPMGFYY361 pKa = 10.68SSWASFAVAHH371 pKa = 6.5HH372 pKa = 5.41YY373 pKa = 9.52VVYY376 pKa = 9.96YY377 pKa = 9.81CCKK380 pKa = 10.41KK381 pKa = 10.84LGIPWKK387 pKa = 7.7TLKK390 pKa = 10.82YY391 pKa = 10.26CLLGDD396 pKa = 5.19DD397 pKa = 5.68IVMCDD402 pKa = 3.57PKK404 pKa = 11.13VAALYY409 pKa = 10.64KK410 pKa = 9.44EE411 pKa = 4.68TISGLGVEE419 pKa = 5.03FSEE422 pKa = 4.83EE423 pKa = 3.79KK424 pKa = 10.4TITSPHH430 pKa = 5.77LVEE433 pKa = 4.07FAKK436 pKa = 10.7RR437 pKa = 11.84LIYY440 pKa = 10.42KK441 pKa = 7.7GTEE444 pKa = 3.65MSPFPISALGEE455 pKa = 4.19LANKK459 pKa = 10.31YY460 pKa = 10.41YY461 pKa = 11.2LLTDD465 pKa = 4.19LFLEE469 pKa = 4.73LEE471 pKa = 4.37SKK473 pKa = 10.6GWVSLGGAPSAVEE486 pKa = 4.16SFHH489 pKa = 7.83KK490 pKa = 10.53IVFNWSTVRR499 pKa = 11.84ARR501 pKa = 11.84EE502 pKa = 4.04IEE504 pKa = 4.49VKK506 pKa = 10.73SSVCEE511 pKa = 3.75RR512 pKa = 11.84VMKK515 pKa = 9.47MARR518 pKa = 11.84GAEE521 pKa = 4.09DD522 pKa = 3.85AGTLLTEE529 pKa = 4.69CFSKK533 pKa = 10.77LGYY536 pKa = 9.88PFVLSSFVASNVLEE550 pKa = 4.09NMVVEE555 pKa = 4.94NFANSNPASHH565 pKa = 6.63NPEE568 pKa = 3.67SDD570 pKa = 3.39KK571 pKa = 11.04KK572 pKa = 11.03SKK574 pKa = 10.57SPCVGLGYY582 pKa = 10.25LAEE585 pKa = 4.65NLTCLLTGLDD595 pKa = 4.37EE596 pKa = 4.67EE597 pKa = 4.91SCSLGFDD604 pKa = 3.51LMMALPHH611 pKa = 6.3LNAYY615 pKa = 9.56GQMSEE620 pKa = 5.0LYY622 pKa = 11.09VNINKK627 pKa = 9.84KK628 pKa = 10.07ARR630 pKa = 11.84QISTTNGAEE639 pKa = 3.83WPLLFKK645 pKa = 9.71TMCIPWDD652 pKa = 3.53DD653 pKa = 4.76TIFTMSSSHH662 pKa = 7.14LIAKK666 pKa = 9.41GSSSVVKK673 pKa = 10.39DD674 pKa = 3.62LQNSAEE680 pKa = 4.43LLSFYY685 pKa = 10.49PPEE688 pKa = 3.96EE689 pKa = 4.18LLRR692 pKa = 11.84EE693 pKa = 4.31DD694 pKa = 4.05PNFLKK699 pKa = 10.93SLL701 pKa = 3.77

Molecular weight: 78.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

701

701

701

701.0

78.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.991 ± 0.0

1.712 ± 0.0

4.993 ± 0.0

5.278 ± 0.0

5.136 ± 0.0

6.562 ± 0.0

1.569 ± 0.0

4.422 ± 0.0

7.561 ± 0.0

11.555 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.994 ± 0.0

4.137 ± 0.0

5.136 ± 0.0

1.997 ± 0.0

2.996 ± 0.0

9.13 ± 0.0

6.277 ± 0.0

5.849 ± 0.0

1.569 ± 0.0

4.137 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski