Salicibibacter kimchii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Salicibibacter

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3509 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345C0U3|A0A345C0U3_9BACI Copper chaperone OS=Salicibibacter kimchii OX=2099786 GN=DT065_12965 PE=4 SV=1
MM1 pKa = 7.17KK2 pKa = 9.99KK3 pKa = 10.46VYY5 pKa = 10.19IGMSTIFFVLAGCSGEE21 pKa = 4.2GLDD24 pKa = 4.5EE25 pKa = 4.86GSTGSTEE32 pKa = 4.08SQQEE36 pKa = 4.25GKK38 pKa = 10.15GNEE41 pKa = 3.94MMTEE45 pKa = 3.91DD46 pKa = 3.5GKK48 pKa = 11.21EE49 pKa = 3.96VFNLDD54 pKa = 3.24VTEE57 pKa = 4.18THH59 pKa = 6.21WMFDD63 pKa = 3.88DD64 pKa = 4.13EE65 pKa = 4.87TMTDD69 pKa = 2.44AWTYY73 pKa = 9.1NGSVPGEE80 pKa = 4.15KK81 pKa = 9.33IRR83 pKa = 11.84VQEE86 pKa = 4.1GDD88 pKa = 3.71EE89 pKa = 4.66VILNVQNNLEE99 pKa = 4.38EE100 pKa = 4.23PTALHH105 pKa = 5.94LHH107 pKa = 6.61GFPVPNAMDD116 pKa = 4.31GVPGVTQNAIMPGEE130 pKa = 4.16EE131 pKa = 3.66FTYY134 pKa = 10.14EE135 pKa = 3.97YY136 pKa = 10.65QADD139 pKa = 3.82VPGTYY144 pKa = 8.57WYY146 pKa = 10.21HH147 pKa = 5.06SHH149 pKa = 6.73QDD151 pKa = 2.59GSTQVDD157 pKa = 3.14QGLYY161 pKa = 10.4GVFIVEE167 pKa = 4.62PEE169 pKa = 4.0DD170 pKa = 3.52QEE172 pKa = 5.41SYY174 pKa = 11.46DD175 pKa = 3.62VDD177 pKa = 3.55EE178 pKa = 5.56VIAIDD183 pKa = 4.06EE184 pKa = 4.38FAPMDD189 pKa = 3.96MDD191 pKa = 4.45MEE193 pKa = 7.08DD194 pKa = 5.29DD195 pKa = 3.36MHH197 pKa = 8.27EE198 pKa = 4.79DD199 pKa = 3.4MDD201 pKa = 5.08HH202 pKa = 8.31SDD204 pKa = 3.79MEE206 pKa = 4.89NGDD209 pKa = 4.14MEE211 pKa = 4.92EE212 pKa = 4.12MDD214 pKa = 4.54HH215 pKa = 8.64ADD217 pKa = 4.13MMNEE221 pKa = 3.99MYY223 pKa = 9.59DD224 pKa = 3.35TMVINGQSSSQIEE237 pKa = 4.37SVDD240 pKa = 3.44VEE242 pKa = 4.34EE243 pKa = 4.61GDD245 pKa = 3.73KK246 pKa = 11.32VKK248 pKa = 10.85LRR250 pKa = 11.84FVNAGLFTQIVSIPEE265 pKa = 3.65HH266 pKa = 6.03AFKK269 pKa = 9.94VTHH272 pKa = 6.73YY273 pKa = 10.59DD274 pKa = 3.45GQPVNEE280 pKa = 4.8PEE282 pKa = 4.46MISDD286 pKa = 3.83TSFQIAPAEE295 pKa = 4.15RR296 pKa = 11.84YY297 pKa = 9.59DD298 pKa = 4.67VEE300 pKa = 5.22IEE302 pKa = 3.91MDD304 pKa = 4.14NPGAWGIQVFAEE316 pKa = 4.19EE317 pKa = 4.0NQEE320 pKa = 4.0RR321 pKa = 11.84LNASLPLIYY330 pKa = 10.23DD331 pKa = 4.2GYY333 pKa = 10.98EE334 pKa = 4.26DD335 pKa = 5.38EE336 pKa = 5.89DD337 pKa = 4.45LQTGDD342 pKa = 4.27TPSSSLDD349 pKa = 3.07LTTYY353 pKa = 11.27GEE355 pKa = 4.18AQNMNEE361 pKa = 3.45YY362 pKa = 10.44DD363 pKa = 3.39INKK366 pKa = 9.43EE367 pKa = 3.42YY368 pKa = 11.4DD369 pKa = 3.47MNLGTDD375 pKa = 3.63DD376 pKa = 4.69GGDD379 pKa = 3.15TFTINGKK386 pKa = 7.75QFPDD390 pKa = 3.28HH391 pKa = 6.85EE392 pKa = 4.52IYY394 pKa = 10.37EE395 pKa = 4.27VEE397 pKa = 4.09EE398 pKa = 4.96GDD400 pKa = 3.97VVKK403 pKa = 9.71VTIEE407 pKa = 3.75NDD409 pKa = 3.51TEE411 pKa = 4.64DD412 pKa = 4.02DD413 pKa = 4.08HH414 pKa = 8.08PMHH417 pKa = 6.57LHH419 pKa = 6.7GEE421 pKa = 4.2FFDD424 pKa = 4.8VISKK428 pKa = 10.68DD429 pKa = 3.62GEE431 pKa = 4.14PLQGSSVTKK440 pKa = 9.47DD441 pKa = 3.32TLNVRR446 pKa = 11.84PGEE449 pKa = 4.02TYY451 pKa = 10.28EE452 pKa = 4.94IVFEE456 pKa = 4.22AQNPGNWMFHH466 pKa = 4.92CHH468 pKa = 5.55EE469 pKa = 4.51FHH471 pKa = 6.84HH472 pKa = 6.93ASDD475 pKa = 3.7GMVAEE480 pKa = 4.21VQYY483 pKa = 11.21EE484 pKa = 4.23GFEE487 pKa = 4.18PAFTPDD493 pKa = 3.86PNVPNQPEE501 pKa = 3.67

Molecular weight: 56.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345BZX9|A0A345BZX9_9BACI Pyridoxal 5'-phosphate synthase subunit PdxS OS=Salicibibacter kimchii OX=2099786 GN=pdxS PE=3 SV=1
MM1 pKa = 7.7AKK3 pKa = 7.99MTFQPNNRR11 pKa = 11.84KK12 pKa = 9.31RR13 pKa = 11.84KK14 pKa = 8.54KK15 pKa = 8.5EE16 pKa = 3.58HH17 pKa = 5.81GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 10.28NGRR29 pKa = 11.84QVLKK33 pKa = 10.51RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.43KK38 pKa = 10.24GRR40 pKa = 11.84KK41 pKa = 8.58NLSAA45 pKa = 4.67

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3509

0

3509

988862

26

1432

281.8

31.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.84 ± 0.039

0.651 ± 0.01

5.849 ± 0.042

8.184 ± 0.065

4.22 ± 0.035

7.253 ± 0.042

2.337 ± 0.018

6.86 ± 0.045

5.289 ± 0.041

9.329 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.038 ± 0.019

3.987 ± 0.025

3.862 ± 0.022

3.833 ± 0.032

4.731 ± 0.037

5.704 ± 0.029

5.574 ± 0.025

7.145 ± 0.033

1.048 ± 0.015

3.265 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski