Mini PCV-like virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; Circovirus; unclassified Circovirus

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R3ZQB5|A0A2R3ZQB5_9CIRC Uncharacterized protein OS=Mini PCV-like virus OX=2137787 PE=4 SV=1
MM1 pKa = 8.08RR2 pKa = 11.84LRR4 pKa = 11.84GNCSCISSHH13 pKa = 6.12ASEE16 pKa = 4.61YY17 pKa = 10.97SSVSHH22 pKa = 6.83FGAGLVLPGTSEE34 pKa = 4.0PGSTEE39 pKa = 3.54VCRR42 pKa = 11.84EE43 pKa = 4.1KK44 pKa = 10.39KK45 pKa = 9.25WYY47 pKa = 9.2PIMDD51 pKa = 4.05APHH54 pKa = 6.65RR55 pKa = 11.84VSGYY59 pKa = 7.99TGQWMMKK66 pKa = 9.29RR67 pKa = 11.84PRR69 pKa = 11.84VLMPQDD75 pKa = 3.93GDD77 pKa = 3.93WITEE81 pKa = 3.89FLWCYY86 pKa = 8.21EE87 pKa = 4.09CPEE90 pKa = 4.73DD91 pKa = 5.3KK92 pKa = 10.72DD93 pKa = 5.28FYY95 pKa = 10.5CHH97 pKa = 7.35PILGPEE103 pKa = 4.79GKK105 pKa = 9.57PEE107 pKa = 4.14TQVVFYY113 pKa = 10.58DD114 pKa = 3.62KK115 pKa = 11.03QLDD118 pKa = 3.66PDD120 pKa = 3.75RR121 pKa = 11.84VGIYY125 pKa = 10.21CGVWRR130 pKa = 11.84QYY132 pKa = 11.39SEE134 pKa = 3.55IPYY137 pKa = 9.13YY138 pKa = 9.82RR139 pKa = 11.84QRR141 pKa = 11.84GWKK144 pKa = 9.2KK145 pKa = 10.27RR146 pKa = 11.84YY147 pKa = 6.11PTLARR152 pKa = 11.84VRR154 pKa = 11.84EE155 pKa = 4.07FQEE158 pKa = 4.01QKK160 pKa = 9.74ATQLGEE166 pKa = 3.7ALTRR170 pKa = 11.84IWSGPSGAGEE180 pKa = 4.33GEE182 pKa = 4.06PQRR185 pKa = 11.84GQRR188 pKa = 11.84VLQEE192 pKa = 4.01RR193 pKa = 11.84GGLPRR198 pKa = 11.84DD199 pKa = 2.99WRR201 pKa = 11.84RR202 pKa = 11.84FLFGYY207 pKa = 10.3QIGSSSSSSDD217 pKa = 2.89SDD219 pKa = 3.83GDD221 pKa = 3.81VGKK224 pKa = 10.87SDD226 pKa = 3.32

Molecular weight: 25.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R3ZQB5|A0A2R3ZQB5_9CIRC Uncharacterized protein OS=Mini PCV-like virus OX=2137787 PE=4 SV=1
MM1 pKa = 7.37FLLVLQDD8 pKa = 3.06AGKK11 pKa = 9.98RR12 pKa = 11.84GKK14 pKa = 9.73LVRR17 pKa = 11.84MRR19 pKa = 11.84LRR21 pKa = 11.84GNCSCISSHH30 pKa = 6.12ASEE33 pKa = 4.61YY34 pKa = 10.97SSVSHH39 pKa = 6.83FGAGLVLPGTSEE51 pKa = 4.0PGSTEE56 pKa = 3.54VCRR59 pKa = 11.84EE60 pKa = 4.1KK61 pKa = 10.39KK62 pKa = 9.25WYY64 pKa = 9.2PIMDD68 pKa = 4.05APHH71 pKa = 6.65RR72 pKa = 11.84VSGYY76 pKa = 7.99TGQWMMKK83 pKa = 9.29RR84 pKa = 11.84PRR86 pKa = 11.84VLMPQDD92 pKa = 3.93GDD94 pKa = 3.93WITEE98 pKa = 3.89FLWCYY103 pKa = 8.21EE104 pKa = 4.09CPEE107 pKa = 4.73DD108 pKa = 5.3KK109 pKa = 10.72DD110 pKa = 5.28FYY112 pKa = 10.5CHH114 pKa = 7.35PILGPEE120 pKa = 4.79GKK122 pKa = 9.57PEE124 pKa = 4.14TQVVFYY130 pKa = 10.58DD131 pKa = 3.62KK132 pKa = 11.03QLDD135 pKa = 3.66PDD137 pKa = 3.75RR138 pKa = 11.84VGIYY142 pKa = 10.21CGVWRR147 pKa = 11.84QYY149 pKa = 11.39SEE151 pKa = 3.55IPYY154 pKa = 9.13YY155 pKa = 9.82RR156 pKa = 11.84QRR158 pKa = 11.84GWKK161 pKa = 9.2KK162 pKa = 10.27RR163 pKa = 11.84YY164 pKa = 6.11PTLARR169 pKa = 11.84VRR171 pKa = 11.84EE172 pKa = 4.07FQEE175 pKa = 4.01QKK177 pKa = 9.74ATQLGEE183 pKa = 3.7ALTRR187 pKa = 11.84IWSGPSGAGEE197 pKa = 4.33GEE199 pKa = 4.06PQRR202 pKa = 11.84GQRR205 pKa = 11.84VLQEE209 pKa = 4.01RR210 pKa = 11.84GGLPRR215 pKa = 11.84DD216 pKa = 2.99WRR218 pKa = 11.84RR219 pKa = 11.84FLFGYY224 pKa = 10.3QIGSSSSSSDD234 pKa = 2.89SDD236 pKa = 3.83GDD238 pKa = 3.81VGKK241 pKa = 10.87SDD243 pKa = 3.32

Molecular weight: 27.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

469

226

243

234.5

26.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.198 ± 0.07

2.985 ± 0.078

5.757 ± 0.003

6.823 ± 0.178

3.198 ± 0.07

11.087 ± 0.018

1.706 ± 0.045

3.412 ± 0.089

4.691 ± 0.185

6.397 ± 0.448

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.345 ± 0.093

0.426 ± 0.011

6.823 ± 0.178

5.757 ± 0.003

8.955 ± 0.073

8.955 ± 0.234

3.412 ± 0.089

5.544 ± 0.163

3.412 ± 0.089

5.117 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski