Anabaena sp. 90

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Anabaena; unclassified Anabaena

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4476 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7WVR6|K7WVR6_9NOST Universal stress protein UspA OS=Anabaena sp. 90 OX=46234 GN=uspA PE=3 SV=1
MM1 pKa = 7.22SQQNLVFTNPNEE13 pKa = 3.8ITIPNGGAATTYY25 pKa = 9.43PSSINVSGLNGTITSLKK42 pKa = 8.6VTLSNISHH50 pKa = 7.46TYY52 pKa = 10.27PDD54 pKa = 4.79DD55 pKa = 5.24LDD57 pKa = 4.09ILLIGPTGANILLMSDD73 pKa = 5.1AGWSWDD79 pKa = 3.77LNNVTLTFDD88 pKa = 3.9PNASDD93 pKa = 4.28FLPDD97 pKa = 3.09QGQISSGSYY106 pKa = 9.51RR107 pKa = 11.84ATDD110 pKa = 3.5YY111 pKa = 9.05EE112 pKa = 4.06TDD114 pKa = 3.47DD115 pKa = 4.17RR116 pKa = 11.84FNNSPAPSGDD126 pKa = 3.36YY127 pKa = 11.3GIDD130 pKa = 3.12FSVFNNTIPNGTWNLYY146 pKa = 9.86VVDD149 pKa = 4.4DD150 pKa = 4.12TGVDD154 pKa = 2.96IGNIAGGWSIAIDD167 pKa = 3.73TTDD170 pKa = 3.18VTTKK174 pKa = 10.52PIVTLATSPKK184 pKa = 9.89KK185 pKa = 10.22VAEE188 pKa = 4.11HH189 pKa = 6.13SGGNILYY196 pKa = 8.45TFTRR200 pKa = 11.84TGNITSALTVNYY212 pKa = 7.95TVSGSAIFNTDD223 pKa = 2.99YY224 pKa = 10.97SQTGATSHH232 pKa = 6.31TATTGTVSFAAGADD246 pKa = 3.75TVTLTISPLEE256 pKa = 4.32DD257 pKa = 3.91NIQEE261 pKa = 3.97NDD263 pKa = 3.04EE264 pKa = 4.49TIILTLATATDD275 pKa = 4.38YY276 pKa = 11.1IIGTTTEE283 pKa = 3.94LTGTITNGNDD293 pKa = 2.78QFEE296 pKa = 4.67ATGSNLVSNKK306 pKa = 9.69IYY308 pKa = 10.67DD309 pKa = 3.9GGGGTDD315 pKa = 3.46TLIVNYY321 pKa = 10.44SSATFSGLGTDD332 pKa = 4.31GLGVSNGYY340 pKa = 8.85WSNSAFHH347 pKa = 7.83DD348 pKa = 3.68YY349 pKa = 10.61RR350 pKa = 11.84GYY352 pKa = 11.21YY353 pKa = 8.81YY354 pKa = 10.57YY355 pKa = 11.09GWRR358 pKa = 11.84ANSPLLSFSNIEE370 pKa = 4.0TFEE373 pKa = 4.16ITGTAYY379 pKa = 10.37ADD381 pKa = 3.66YY382 pKa = 10.96LRR384 pKa = 11.84GYY386 pKa = 10.34SGDD389 pKa = 3.78DD390 pKa = 3.26KK391 pKa = 11.65LIGGEE396 pKa = 4.32GNDD399 pKa = 5.23DD400 pKa = 3.39IAGGDD405 pKa = 3.84GNDD408 pKa = 5.02LIRR411 pKa = 11.84GGDD414 pKa = 3.69GNDD417 pKa = 3.33TLKK420 pKa = 11.45GEE422 pKa = 4.43GGNDD426 pKa = 3.49EE427 pKa = 4.85IEE429 pKa = 4.71GGDD432 pKa = 3.99GNDD435 pKa = 4.2TIDD438 pKa = 3.72GGTGRR443 pKa = 11.84HH444 pKa = 5.61VIRR447 pKa = 11.84GGTGNDD453 pKa = 2.98TVAGAKK459 pKa = 10.36AGDD462 pKa = 4.27SIDD465 pKa = 4.17GEE467 pKa = 4.62GGNDD471 pKa = 3.42TLQSLDD477 pKa = 3.83LSSLGVNLVLDD488 pKa = 4.29LRR490 pKa = 11.84ATEE493 pKa = 4.25QVSGDD498 pKa = 3.16NNTRR502 pKa = 11.84INNFEE507 pKa = 4.63NISSVTLGSGHH518 pKa = 7.23DD519 pKa = 3.6TTIATSNLLVSNGSINAGGGTDD541 pKa = 3.8RR542 pKa = 11.84LIVDD546 pKa = 3.73YY547 pKa = 11.33SSATFSGLGTDD558 pKa = 4.31GLGVSNGYY566 pKa = 8.85WSNSAFHH573 pKa = 7.83DD574 pKa = 3.68YY575 pKa = 10.61RR576 pKa = 11.84GYY578 pKa = 11.21YY579 pKa = 8.81YY580 pKa = 10.57YY581 pKa = 11.09GWRR584 pKa = 11.84ANSPLLSFSNIEE596 pKa = 4.0TFEE599 pKa = 4.16ITGTAYY605 pKa = 10.37ADD607 pKa = 3.66YY608 pKa = 10.96LRR610 pKa = 11.84GYY612 pKa = 10.34SGDD615 pKa = 3.78DD616 pKa = 3.26KK617 pKa = 11.65LIGGEE622 pKa = 4.32GNDD625 pKa = 5.23DD626 pKa = 3.39IAGGDD631 pKa = 3.84GNDD634 pKa = 5.02LIRR637 pKa = 11.84GGDD640 pKa = 3.69GNDD643 pKa = 3.33TLKK646 pKa = 11.45GEE648 pKa = 4.43GGNDD652 pKa = 3.49EE653 pKa = 4.85IEE655 pKa = 4.71GGDD658 pKa = 3.99GNDD661 pKa = 4.2TIDD664 pKa = 3.78GGTGRR669 pKa = 11.84HH670 pKa = 6.9LIRR673 pKa = 11.84GGKK676 pKa = 10.1GDD678 pKa = 3.92DD679 pKa = 3.45TVVSAKK685 pKa = 10.69AGDD688 pKa = 4.28SIDD691 pKa = 3.74GGEE694 pKa = 4.76GNDD697 pKa = 3.73TLQSLDD703 pKa = 4.14LSSLDD708 pKa = 3.16VDD710 pKa = 4.29LVLNLRR716 pKa = 11.84VTEE719 pKa = 4.01QLSGDD724 pKa = 3.37NNTRR728 pKa = 11.84INNFEE733 pKa = 4.55NISTVTLGSGYY744 pKa = 8.83DD745 pKa = 3.36TTIATGNLLVSNGSFNGGGGTDD767 pKa = 3.65TLIVDD772 pKa = 3.87YY773 pKa = 11.3SSATFSGLGTDD784 pKa = 4.31GLGVSNGYY792 pKa = 8.85WSNSAFHH799 pKa = 7.83DD800 pKa = 3.68YY801 pKa = 10.61RR802 pKa = 11.84GYY804 pKa = 11.21YY805 pKa = 8.81YY806 pKa = 10.57YY807 pKa = 11.09GWRR810 pKa = 11.84ANSPLLSFSNIEE822 pKa = 4.0TFEE825 pKa = 4.16ITGTAYY831 pKa = 10.37ADD833 pKa = 3.66YY834 pKa = 10.96LRR836 pKa = 11.84GYY838 pKa = 10.34SGDD841 pKa = 3.78DD842 pKa = 3.26KK843 pKa = 11.65LIGGEE848 pKa = 4.27GNDD851 pKa = 4.26NIAGGDD857 pKa = 3.65GSDD860 pKa = 3.89TIIPVNPNVAKK871 pKa = 10.42PGLGTVDD878 pKa = 3.48TVTGGTGEE886 pKa = 4.15DD887 pKa = 3.61TFILGDD893 pKa = 3.97ATWIGYY899 pKa = 9.95DD900 pKa = 4.32DD901 pKa = 4.49YY902 pKa = 12.02NSTTAGTSDD911 pKa = 3.41YY912 pKa = 11.26LIIKK916 pKa = 9.25DD917 pKa = 4.26FNPLEE922 pKa = 5.49DD923 pKa = 4.57IIQLLGSSSDD933 pKa = 3.52YY934 pKa = 8.07TTTVSGNNINLYY946 pKa = 9.87INKK949 pKa = 8.93PDD951 pKa = 3.84SEE953 pKa = 4.53PDD955 pKa = 3.07EE956 pKa = 5.1LIAVLEE962 pKa = 4.17NTQGLINSLQFNLTGSYY979 pKa = 10.26FKK981 pKa = 11.04YY982 pKa = 9.66IASNAVLNITSTSTSRR998 pKa = 11.84IQTEE1002 pKa = 3.99GDD1004 pKa = 3.28SDD1006 pKa = 3.61NKK1008 pKa = 10.96SFVFTVTRR1016 pKa = 11.84TGDD1019 pKa = 3.12ASGTSTVDD1027 pKa = 2.88WFITPSGDD1035 pKa = 3.36NPVNSTDD1042 pKa = 3.89FFGGAIPPGEE1052 pKa = 4.41SLTFLPGEE1060 pKa = 3.98TSKK1063 pKa = 10.86EE1064 pKa = 3.27IRR1066 pKa = 11.84VRR1068 pKa = 11.84VRR1070 pKa = 11.84GDD1072 pKa = 3.14SAIEE1076 pKa = 4.05SNEE1079 pKa = 3.7TFTVYY1084 pKa = 10.7LSTPSRR1090 pKa = 11.84STIGTRR1096 pKa = 11.84SATGIILNDD1105 pKa = 4.64DD1106 pKa = 4.13GSQPTLPVIGTSGNDD1121 pKa = 3.58TLSGTINADD1130 pKa = 3.56CLIGLAGNDD1139 pKa = 3.3TYY1141 pKa = 11.32TVNNAGDD1148 pKa = 3.78VVVEE1152 pKa = 4.32NPSEE1156 pKa = 4.0GTDD1159 pKa = 2.94RR1160 pKa = 11.84VNASISYY1167 pKa = 8.49TLADD1171 pKa = 3.38NVEE1174 pKa = 4.07NLTLTGSANLNGTGNDD1190 pKa = 3.96LNNSLTGNTGNNILTGNAGNDD1211 pKa = 3.92TLNGAAGIDD1220 pKa = 3.75TLIGGLGDD1228 pKa = 4.75DD1229 pKa = 4.26IYY1231 pKa = 11.77VVDD1234 pKa = 4.46STTDD1238 pKa = 3.39TITEE1242 pKa = 4.03NSGEE1246 pKa = 4.17GTDD1249 pKa = 4.41IIQSSVTFDD1258 pKa = 3.04LTVFPNIEE1266 pKa = 4.16NLTLTGTAVINGTGNAGDD1284 pKa = 3.82NSLTGNSGNNTLTGNAGNDD1303 pKa = 3.77LLNGGTGNDD1312 pKa = 4.3LLNGGAGADD1321 pKa = 3.8TLTGGIGSDD1330 pKa = 3.13TLVFQFGQSPVSGADD1345 pKa = 3.31RR1346 pKa = 11.84ITDD1349 pKa = 3.92FAIGTDD1355 pKa = 4.05KK1356 pKa = 10.9IDD1358 pKa = 3.9LLTSLGVAMNAPTAFTRR1375 pKa = 11.84AANSTDD1381 pKa = 3.17TTLTNVVNSVFTDD1394 pKa = 3.36ANGALTDD1401 pKa = 3.81NQALGVSSAALVSVTTSGIAGTYY1424 pKa = 10.11LVINDD1429 pKa = 4.34GVAGFQSSNDD1439 pKa = 3.44LLVNITGSSGTLPALGTIAVSSFFII1464 pKa = 4.76

Molecular weight: 151.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7W0S5|K7W0S5_9NOST Non-specific serine/threonine protein kinase OS=Anabaena sp. 90 OX=46234 GN=ANA_C13407 PE=4 SV=1
MM1 pKa = 6.44QQRR4 pKa = 11.84FAIASLQLPKK14 pKa = 10.17SDD16 pKa = 5.19RR17 pKa = 11.84IPSTSQTANCLQQRR31 pKa = 11.84FAIAPPQPSKK41 pKa = 10.46QRR43 pKa = 11.84RR44 pKa = 11.84TPSTSQTANCLQQRR58 pKa = 11.84FAIAPLNPPNSEE70 pKa = 4.1LLAAALRR77 pKa = 11.84YY78 pKa = 7.67RR79 pKa = 11.84TPQPPKK85 pKa = 10.37QRR87 pKa = 11.84TACSSASLSHH97 pKa = 7.03PLNLPNSEE105 pKa = 4.46LLAAALRR112 pKa = 11.84YY113 pKa = 7.53RR114 pKa = 11.84TSSNFPNILFLTNHH128 pKa = 7.15RR129 pKa = 11.84DD130 pKa = 3.54TEE132 pKa = 4.25DD133 pKa = 3.52TEE135 pKa = 4.63KK136 pKa = 10.84EE137 pKa = 4.05RR138 pKa = 11.84SHH140 pKa = 7.33SLL142 pKa = 3.18

Molecular weight: 15.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4476

0

4476

1402120

28

5062

313.3

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.349 ± 0.036

0.995 ± 0.013

4.938 ± 0.025

6.363 ± 0.034

4.036 ± 0.024

6.446 ± 0.036

1.843 ± 0.017

7.498 ± 0.03

5.494 ± 0.039

10.836 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.842 ± 0.019

5.012 ± 0.036

4.53 ± 0.024

5.305 ± 0.037

4.569 ± 0.029

6.35 ± 0.028

5.741 ± 0.037

6.27 ± 0.03

1.403 ± 0.016

3.182 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski