Firmicutes bacterium CAG:83

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1718 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5DDB9|R5DDB9_9FIRM Serine-type D-Ala-D-Ala carboxypeptidase OS=Firmicutes bacterium CAG:83 OX=1262992 GN=BN795_01696 PE=4 SV=1
MM1 pKa = 6.89NWKK4 pKa = 8.53EE5 pKa = 3.79TFYY8 pKa = 10.35TGKK11 pKa = 10.04RR12 pKa = 11.84EE13 pKa = 4.09HH14 pKa = 6.45PQEE17 pKa = 4.33EE18 pKa = 4.85KK19 pKa = 10.36PASLTALLMPSLLGVAICLVCLCSLTWAWFTATQNSGVQPIQSATATVTAKK70 pKa = 11.01LNDD73 pKa = 3.69TALGEE78 pKa = 4.33LKK80 pKa = 10.79VGEE83 pKa = 4.26PCNLYY88 pKa = 9.0GTGTLTLHH96 pKa = 6.28MAGDD100 pKa = 3.61AQYY103 pKa = 10.24AYY105 pKa = 11.11VVIKK109 pKa = 11.07VGDD112 pKa = 3.87TEE114 pKa = 4.29YY115 pKa = 9.09HH116 pKa = 5.7TDD118 pKa = 3.33YY119 pKa = 10.69LTANKK124 pKa = 10.34DD125 pKa = 3.27YY126 pKa = 10.26TITVNEE132 pKa = 4.19SGAALTLCWGKK143 pKa = 10.18QDD145 pKa = 4.31SLPGTAVGDD154 pKa = 3.75GGSIGTAQPSNTTDD168 pKa = 3.06EE169 pKa = 4.35QPAVNGDD176 pKa = 3.68SQQTPDD182 pKa = 3.79AAVPGADD189 pKa = 4.48DD190 pKa = 5.66ANTGDD195 pKa = 3.51QTSNGEE201 pKa = 4.11NTGSEE206 pKa = 4.3NGGSTGNGSGEE217 pKa = 4.07QGSDD221 pKa = 2.81SGTGSDD227 pKa = 3.54TGAGGSTGGDD237 pKa = 3.14AGTGDD242 pKa = 4.09TSNGTDD248 pKa = 3.23GTTGEE253 pKa = 4.26

Molecular weight: 25.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5D057|R5D057_9FIRM WYL domain-containing protein OS=Firmicutes bacterium CAG:83 OX=1262992 GN=BN795_00003 PE=4 SV=1
MM1 pKa = 7.25FRR3 pKa = 11.84TYY5 pKa = 9.97QPKK8 pKa = 9.59KK9 pKa = 7.61RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.69EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 10.07RR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.04GRR39 pKa = 11.84ARR41 pKa = 11.84LTHH44 pKa = 6.28

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1718

0

1718

518289

30

2276

301.7

33.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.553 ± 0.066

1.772 ± 0.029

5.487 ± 0.046

6.588 ± 0.056

3.457 ± 0.037

8.248 ± 0.079

2.076 ± 0.033

5.253 ± 0.05

4.217 ± 0.06

10.334 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.902 ± 0.031

2.895 ± 0.041

4.288 ± 0.037

3.743 ± 0.04

6.22 ± 0.069

5.212 ± 0.045

5.742 ± 0.055

7.567 ± 0.048

1.03 ± 0.021

3.412 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski