Mycobacterium phage Vincenzo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Coopervirus; Mycobacterium virus Vincenzo

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6SJJ8|A0A0F6SJJ8_9CAUD Uncharacterized protein OS=Mycobacterium phage Vincenzo OX=1647301 GN=80 PE=4 SV=1
MM1 pKa = 7.47SSNVTVIMATDD12 pKa = 3.53NNQGYY17 pKa = 9.09AADD20 pKa = 3.87QVSTDD25 pKa = 3.3MTLADD30 pKa = 4.69LLAAVQEE37 pKa = 4.91AIDD40 pKa = 4.26TYY42 pKa = 11.63GEE44 pKa = 4.01DD45 pKa = 3.41AKK47 pKa = 11.43VVTKK51 pKa = 10.81DD52 pKa = 2.55SGQRR56 pKa = 11.84YY57 pKa = 7.47GANFGRR63 pKa = 11.84LGGAYY68 pKa = 10.09DD69 pKa = 3.42QDD71 pKa = 3.93LFEE74 pKa = 5.89DD75 pKa = 3.98ADD77 pKa = 4.52APAEE81 pKa = 4.34DD82 pKa = 3.93YY83 pKa = 11.27GYY85 pKa = 11.69

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6WE14|A0A0F6WE14_9CAUD Uncharacterized protein OS=Mycobacterium phage Vincenzo OX=1647301 GN=82 PE=4 SV=1
MM1 pKa = 7.83AKK3 pKa = 9.26TKK5 pKa = 10.24ARR7 pKa = 11.84KK8 pKa = 6.33GTRR11 pKa = 11.84RR12 pKa = 11.84AGALRR17 pKa = 11.84GGTRR21 pKa = 11.84KK22 pKa = 9.23IARR25 pKa = 11.84SRR27 pKa = 11.84RR28 pKa = 11.84PGTIALHH35 pKa = 6.14AKK37 pKa = 10.1AGGIATFAKK46 pKa = 10.38GGGGGYY52 pKa = 10.11RR53 pKa = 11.84GFKK56 pKa = 10.23SKK58 pKa = 10.67KK59 pKa = 5.54QWRR62 pKa = 11.84WAWATKK68 pKa = 9.83QPWARR73 pKa = 11.84KK74 pKa = 9.06KK75 pKa = 9.68SHH77 pKa = 5.63EE78 pKa = 4.29TAGGPKK84 pKa = 9.39VRR86 pKa = 11.84YY87 pKa = 9.27RR88 pKa = 11.84RR89 pKa = 11.84LPASKK94 pKa = 10.39HH95 pKa = 5.8SGHH98 pKa = 6.84KK99 pKa = 8.47GTRR102 pKa = 11.84RR103 pKa = 11.84PKK105 pKa = 10.02SS106 pKa = 3.28

Molecular weight: 11.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

22993

43

2009

234.6

25.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.195 ± 0.33

1.005 ± 0.165

6.95 ± 0.324

4.745 ± 0.284

2.349 ± 0.1

9.69 ± 0.779

1.909 ± 0.17

3.836 ± 0.204

2.792 ± 0.169

8.072 ± 0.221

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.14 ± 0.105

2.87 ± 0.193

6.237 ± 0.292

3.527 ± 0.163

6.245 ± 0.349

4.758 ± 0.193

7.467 ± 0.217

7.972 ± 0.271

1.874 ± 0.155

2.366 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski