Vagococcus fessus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2111 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A430A820|A0A430A820_9ENTE Fructose-1 6-bisphosphatase class 3 OS=Vagococcus fessus OX=120370 GN=fbp PE=3 SV=1
MM1 pKa = 7.77IEE3 pKa = 3.82ATFKK7 pKa = 10.74RR8 pKa = 11.84RR9 pKa = 11.84ATGQIVSFEE18 pKa = 4.27LSGHH22 pKa = 6.18ANSGPYY28 pKa = 10.44GSDD31 pKa = 2.72IVCAGVSALAFNTVNSIDD49 pKa = 3.66ALAGFTPIVEE59 pKa = 3.92VDD61 pKa = 3.74AVDD64 pKa = 3.23EE65 pKa = 5.08GYY67 pKa = 11.01LYY69 pKa = 11.15LEE71 pKa = 4.73TYY73 pKa = 11.25DD74 pKa = 5.99DD75 pKa = 3.74ITDD78 pKa = 3.65EE79 pKa = 4.25QSAKK83 pKa = 10.59SQLLLEE89 pKa = 4.32SLVLGLQGLQEE100 pKa = 4.33EE101 pKa = 4.77YY102 pKa = 11.54ADD104 pKa = 4.69FISLKK109 pKa = 9.9TINNN113 pKa = 3.65

Molecular weight: 12.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A430A9A3|A0A430A9A3_9ENTE Chromosomal replication initiator protein DnaA OS=Vagococcus fessus OX=120370 GN=dnaA PE=3 SV=1
MM1 pKa = 7.13AQQRR5 pKa = 11.84RR6 pKa = 11.84GGGRR10 pKa = 11.84KK11 pKa = 8.53RR12 pKa = 11.84RR13 pKa = 11.84KK14 pKa = 9.0VCYY17 pKa = 9.77FCANHH22 pKa = 7.07ADD24 pKa = 3.42HH25 pKa = 7.15VDD27 pKa = 3.63YY28 pKa = 11.48KK29 pKa = 10.91EE30 pKa = 4.11VEE32 pKa = 4.08LLKK35 pKa = 10.96SRR37 pKa = 11.84FISEE41 pKa = 3.98RR42 pKa = 11.84GKK44 pKa = 9.78ILPRR48 pKa = 11.84RR49 pKa = 11.84VTGTCAKK56 pKa = 9.69HH57 pKa = 5.69QRR59 pKa = 11.84TLTVAIKK66 pKa = 10.14RR67 pKa = 11.84ARR69 pKa = 11.84IMGLLPFVADD79 pKa = 3.92EE80 pKa = 4.04QQ81 pKa = 3.97

Molecular weight: 9.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2111

0

2111

666555

37

1639

315.8

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.705 ± 0.058

0.701 ± 0.016

5.526 ± 0.039

7.792 ± 0.065

4.335 ± 0.041

6.689 ± 0.06

1.69 ± 0.021

7.56 ± 0.053

7.509 ± 0.052

9.861 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.798 ± 0.026

4.564 ± 0.037

3.45 ± 0.035

3.396 ± 0.033

3.511 ± 0.038

6.286 ± 0.039

6.064 ± 0.043

7.191 ± 0.05

0.809 ± 0.015

3.562 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski