Flavobacterium phage vB_FspS_laban6-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Labanvirus; Flavobacterium virus Laban

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9LJA4|A0A6B9LJA4_9CAUD Transcriptional regulator OS=Flavobacterium phage vB_FspS_laban6-1 OX=2686250 GN=laban61_gp054 PE=4 SV=1
MM1 pKa = 7.25IVYY4 pKa = 9.91RR5 pKa = 11.84VYY7 pKa = 11.27NDD9 pKa = 3.01NGGYY13 pKa = 8.38TEE15 pKa = 4.6FAEE18 pKa = 4.72EE19 pKa = 3.84PQGVNFEE26 pKa = 4.78IIYY29 pKa = 8.49KK30 pKa = 7.99TLEE33 pKa = 4.46EE34 pKa = 4.28ISDD37 pKa = 3.9ANKK40 pKa = 10.13PIIPDD45 pKa = 4.41TISVIPFFVQLEE57 pKa = 4.25LMGITEE63 pKa = 4.03QDD65 pKa = 3.09IVDD68 pKa = 4.72KK69 pKa = 10.77IIEE72 pKa = 4.06LHH74 pKa = 5.7QLGILSNKK82 pKa = 9.15EE83 pKa = 3.71KK84 pKa = 10.58IEE86 pKa = 3.73ALISVKK92 pKa = 10.36RR93 pKa = 11.84ATKK96 pKa = 10.1FEE98 pKa = 4.19RR99 pKa = 11.84SHH101 pKa = 6.09PFIGIVAQAFNISEE115 pKa = 4.33SQVDD119 pKa = 4.04EE120 pKa = 4.05IFINGNIFF128 pKa = 3.13

Molecular weight: 14.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9LC71|A0A6B9LC71_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspS_laban6-1 OX=2686250 GN=laban61_gp032 PE=4 SV=1
MM1 pKa = 7.21KK2 pKa = 9.99QLKK5 pKa = 8.73VVCSARR11 pKa = 11.84RR12 pKa = 11.84LGGAKK17 pKa = 9.59RR18 pKa = 11.84YY19 pKa = 9.64RR20 pKa = 11.84NHH22 pKa = 6.87ISIKK26 pKa = 10.53KK27 pKa = 8.26PFDD30 pKa = 3.82VITPTRR36 pKa = 11.84TSGAVFLLKK45 pKa = 10.35HH46 pKa = 4.81SHH48 pKa = 6.76FNLFF52 pKa = 3.34

Molecular weight: 5.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13918

30

1418

244.2

28.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.64 ± 0.479

0.812 ± 0.118

5.461 ± 0.207

7.594 ± 0.313

5.64 ± 0.298

5.008 ± 0.338

1.724 ± 0.206

7.975 ± 0.408

9.24 ± 0.395

8.859 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.558 ± 0.166

6.545 ± 0.237

3.104 ± 0.276

3.772 ± 0.294

3.528 ± 0.217

6.696 ± 0.21

5.532 ± 0.357

5.166 ± 0.212

1.135 ± 0.11

4.009 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski