Acinetobacter phage vB_AbaM_D22

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Saclayvirus; unclassified Saclayvirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H0XAW9|A0A6H0XAW9_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_D22 OX=2724308 GN=vBAbaMD22_64 PE=4 SV=1
MM1 pKa = 7.6TINVDD6 pKa = 3.77LKK8 pKa = 9.49YY9 pKa = 10.71GSRR12 pKa = 11.84SIYY15 pKa = 10.09KK16 pKa = 10.28VLDD19 pKa = 3.99DD20 pKa = 4.49EE21 pKa = 6.17DD22 pKa = 3.64GHH24 pKa = 7.48KK25 pKa = 10.94YY26 pKa = 9.73LVPYY30 pKa = 10.49GEE32 pKa = 4.05YY33 pKa = 10.89DD34 pKa = 3.15EE35 pKa = 6.7VYY37 pKa = 10.9DD38 pKa = 3.64QFQNILEE45 pKa = 4.25AQEE48 pKa = 4.58DD49 pKa = 3.9DD50 pKa = 4.68LYY52 pKa = 11.76SQIEE56 pKa = 4.32EE57 pKa = 4.15LLAPYY62 pKa = 9.74EE63 pKa = 4.13RR64 pKa = 11.84LEE66 pKa = 4.31GADD69 pKa = 3.91FLVILQSEE77 pKa = 4.83VISVGII83 pKa = 3.97

Molecular weight: 9.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H0XAV3|A0A6H0XAV3_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_D22 OX=2724308 GN=vBAbaMD22_45 PE=4 SV=1
MM1 pKa = 7.58TIVLIQIIIALFFLINLYY19 pKa = 10.98CEE21 pKa = 4.08VSKK24 pKa = 10.76KK25 pKa = 10.33YY26 pKa = 10.42RR27 pKa = 11.84NCEE30 pKa = 3.71LHH32 pKa = 6.98VSLRR36 pKa = 11.84WIFMIASLVALAKK49 pKa = 9.85VHH51 pKa = 6.4ILAAMPVVVQISALIIVIAIAKK73 pKa = 9.45ILYY76 pKa = 9.76DD77 pKa = 3.69HH78 pKa = 6.01VLKK81 pKa = 10.77RR82 pKa = 11.84QIRR85 pKa = 11.84IRR87 pKa = 11.84NFKK90 pKa = 10.27LKK92 pKa = 10.4KK93 pKa = 10.31LYY95 pKa = 11.16LNDD98 pKa = 4.0FQKK101 pKa = 9.48HH102 pKa = 3.77THH104 pKa = 5.67RR105 pKa = 5.58

Molecular weight: 12.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

159

0

159

30486

37

2211

191.7

21.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.196 ± 0.325

1.233 ± 0.112

6.721 ± 0.179

7.364 ± 0.228

4.507 ± 0.187

6.734 ± 0.155

1.971 ± 0.158

6.606 ± 0.147

7.909 ± 0.269

7.807 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.726 ± 0.128

4.93 ± 0.162

2.913 ± 0.112

3.395 ± 0.17

4.356 ± 0.145

6.364 ± 0.228

5.609 ± 0.254

6.984 ± 0.169

1.388 ± 0.073

4.287 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski