Methylibium sp. NZG

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Methylibium; unclassified Methylibium

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3979 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L6T4U4|A0A0L6T4U4_9BURK Sugar ABC transporter permease OS=Methylibium sp. NZG OX=1686064 GN=AD742_18355 PE=3 SV=1
MM1 pKa = 7.28HH2 pKa = 7.36QYY4 pKa = 10.3QCVVCGFIYY13 pKa = 10.65DD14 pKa = 4.09EE15 pKa = 4.4AVGMPDD21 pKa = 3.02EE22 pKa = 5.41GIPAGTRR29 pKa = 11.84WADD32 pKa = 3.34IPADD36 pKa = 3.98WACPDD41 pKa = 4.11CGVAKK46 pKa = 10.55ADD48 pKa = 3.51FDD50 pKa = 4.22MVQLL54 pKa = 4.15

Molecular weight: 5.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L6TB11|A0A0L6TB11_9BURK 3-dehydroquinate dehydratase OS=Methylibium sp. NZG OX=1686064 GN=aroQ PE=3 SV=1
MM1 pKa = 7.41WLEE4 pKa = 4.14PQTQTRR10 pKa = 11.84LQRR13 pKa = 11.84QRR15 pKa = 11.84QRR17 pKa = 11.84QRR19 pKa = 11.84QRR21 pKa = 11.84QLQFQRR27 pKa = 11.84QLRR30 pKa = 11.84LRR32 pKa = 11.84LRR34 pKa = 11.84LRR36 pKa = 11.84LLLRR40 pKa = 11.84LRR42 pKa = 11.84LRR44 pKa = 11.84LRR46 pKa = 11.84LRR48 pKa = 11.84LRR50 pKa = 11.84LRR52 pKa = 11.84LRR54 pKa = 11.84LRR56 pKa = 11.84LRR58 pKa = 11.84LQALTAWGEE67 pKa = 3.97PP68 pKa = 3.75

Molecular weight: 8.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3979

0

3979

1311359

32

4961

329.6

35.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.628 ± 0.062

0.908 ± 0.013

5.23 ± 0.033

4.957 ± 0.04

3.51 ± 0.025

8.555 ± 0.052

2.268 ± 0.026

3.957 ± 0.024

3.187 ± 0.039

10.652 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.024

2.508 ± 0.029

5.407 ± 0.028

3.747 ± 0.025

7.0 ± 0.043

5.3 ± 0.038

5.31 ± 0.047

8.0 ± 0.034

1.474 ± 0.017

1.999 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski