Hubei macula-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KJV4|A0A1L3KJV4_9VIRU Uncharacterized protein OS=Hubei macula-like virus 2 OX=1922923 PE=4 SV=1
MM1 pKa = 6.94NTLLQTAIEE10 pKa = 4.21HH11 pKa = 6.22APAIISALPSLLPPSSQSTSTSSDD35 pKa = 3.26PVHH38 pKa = 7.91DD39 pKa = 5.4DD40 pKa = 3.47RR41 pKa = 11.84LLLANSALPPAPSTPSHH58 pKa = 6.38ISSFPSSSSVKK69 pKa = 10.46VPFQYY74 pKa = 10.99VWFGLDD80 pKa = 3.15GTEE83 pKa = 4.65SKK85 pKa = 10.5NASITIPEE93 pKa = 4.1IPSVKK98 pKa = 10.18KK99 pKa = 10.34IIAFFRR105 pKa = 11.84DD106 pKa = 3.33AKK108 pKa = 10.63LVNLEE113 pKa = 3.97AAVYY117 pKa = 8.67PHH119 pKa = 7.03FDD121 pKa = 3.85CLEE124 pKa = 4.01KK125 pKa = 10.62PITVDD130 pKa = 4.84LMWSPSDD137 pKa = 3.68TVPSGSILNCPGAVCFTVGGQFLSQSGILPCDD169 pKa = 3.84LNFMNPIIKK178 pKa = 10.41SPIPYY183 pKa = 8.88TNHH186 pKa = 6.0PRR188 pKa = 11.84LNAKK192 pKa = 9.7FYY194 pKa = 11.17KK195 pKa = 10.4NQTEE199 pKa = 4.33LQSHH203 pKa = 6.51RR204 pKa = 11.84KK205 pKa = 7.15ATIIIRR211 pKa = 11.84GIIACAHH218 pKa = 5.19PTVYY222 pKa = 10.85AA223 pKa = 4.13

Molecular weight:
24.09 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KJV4|A0A1L3KJV4_9VIRU Uncharacterized protein OS=Hubei macula-like virus 2 OX=1922923 PE=4 SV=1
MM1 pKa = 7.65SSLSNNMQILSNTLHH16 pKa = 7.02KK17 pKa = 10.71DD18 pKa = 3.19ASTHH22 pKa = 6.19PLLTHH27 pKa = 5.67TLKK30 pKa = 10.8NLHH33 pKa = 6.26TSLHH37 pKa = 5.81AFPWSLTSEE46 pKa = 4.21QVEE49 pKa = 4.15LLVNLGINVSPHH61 pKa = 4.72GTLPHH66 pKa = 6.16PHH68 pKa = 7.12PVHH71 pKa = 6.5KK72 pKa = 10.63CIEE75 pKa = 4.12THH77 pKa = 6.21VLFNHH82 pKa = 6.3WNHH85 pKa = 6.76LAVQPSAVCFMKK97 pKa = 10.38PSKK100 pKa = 9.85FAKK103 pKa = 10.19LSNSNPNFIEE113 pKa = 3.78LHH115 pKa = 6.05NYY117 pKa = 9.43IIDD120 pKa = 3.7ARR122 pKa = 11.84DD123 pKa = 3.39PTRR126 pKa = 11.84YY127 pKa = 9.35PSISSSLPTTPYY139 pKa = 11.17LFIHH143 pKa = 7.15DD144 pKa = 4.48ALMFISPNEE153 pKa = 4.19IIDD156 pKa = 4.46LFSNCPNLTHH166 pKa = 6.97VFGTLVLPAEE176 pKa = 4.94SIHH179 pKa = 6.42NIPSFYY185 pKa = 10.07PDD187 pKa = 2.93IYY189 pKa = 10.43TYY191 pKa = 10.71HH192 pKa = 6.89HH193 pKa = 6.94EE194 pKa = 4.5NGNLHH199 pKa = 6.39YY200 pKa = 9.97FLEE203 pKa = 5.26GNPSPHH209 pKa = 5.19YY210 pKa = 7.7TQPKK214 pKa = 9.34DD215 pKa = 3.14AAIWLTTHH223 pKa = 7.56TINGPNFSLSVVILEE238 pKa = 4.39SWLSVHH244 pKa = 7.05SILITKK250 pKa = 7.11YY251 pKa = 8.0QPRR254 pKa = 11.84VPTYY258 pKa = 8.99KK259 pKa = 10.25HH260 pKa = 5.71YY261 pKa = 10.78FRR263 pKa = 11.84IPKK266 pKa = 9.42TSLLPNPTNLNLPLHH281 pKa = 6.16SRR283 pKa = 11.84LVPTEE288 pKa = 3.57VHH290 pKa = 6.58ASLFNYY296 pKa = 8.49VRR298 pKa = 11.84AVRR301 pKa = 11.84TLRR304 pKa = 11.84TSDD307 pKa = 3.08PSAFIRR313 pKa = 11.84SLRR316 pKa = 11.84SKK318 pKa = 10.86EE319 pKa = 3.8EE320 pKa = 3.84YY321 pKa = 9.87RR322 pKa = 11.84WVHH325 pKa = 5.97ASAWDD330 pKa = 3.6QLVQFALATHH340 pKa = 6.44NVTSPSLFAFIEE352 pKa = 4.27TFLRR356 pKa = 11.84QILNHH361 pKa = 6.63AKK363 pKa = 9.04LTFLQFIPNPIHH375 pKa = 7.1HH376 pKa = 6.91AFFWLLAKK384 pKa = 10.16ALSYY388 pKa = 8.97ITPKK392 pKa = 10.04PYY394 pKa = 9.56STIFHH399 pKa = 5.94VASIISPLLHH409 pKa = 6.29TYY411 pKa = 8.99WLFSHH416 pKa = 7.45PSPPQVILANYY427 pKa = 7.89NQYY430 pKa = 10.45FHH432 pKa = 7.83PDD434 pKa = 3.0PWLLFADD441 pKa = 4.96RR442 pKa = 11.84YY443 pKa = 10.85SIVAMPKK450 pKa = 9.66PFLSLTLQTPPPSPTSTPPPQPTQQTQPALPAPPPVQTSNPLNSTPFAPPSSLPPLQTPQSAQPTILSTSTPVLPPIVSPPITTCNNPDD539 pKa = 4.14PLLTDD544 pKa = 3.8PTAIGVILPFNQLYY558 pKa = 9.79PGLSMPSHH566 pKa = 6.2VCNFPSRR573 pKa = 11.84LRR575 pKa = 11.84DD576 pKa = 3.44PNAPLPSYY584 pKa = 10.48PITQVCLFDD593 pKa = 4.64ALSTLLQQPQHH604 pKa = 6.19NLFQILAQYY613 pKa = 10.61LPDD616 pKa = 3.82SLVVGPEE623 pKa = 4.05EE624 pKa = 4.18KK625 pKa = 10.11TSGYY629 pKa = 8.66STDD632 pKa = 3.74HH633 pKa = 7.16LDD635 pKa = 2.94ILLYY639 pKa = 10.9HH640 pKa = 6.95FDD642 pKa = 3.55QPLIIHH648 pKa = 6.62SSLGDD653 pKa = 3.62EE654 pKa = 3.92PHH656 pKa = 6.6GPKK659 pKa = 10.16NSRR662 pKa = 11.84NLLHH666 pKa = 7.15LYY668 pKa = 10.03HH669 pKa = 6.35FTGHH673 pKa = 6.66WSAQPPSQPPLRR685 pKa = 11.84GSGLSPFANAVLSFRR700 pKa = 11.84CEE702 pKa = 4.2DD703 pKa = 3.46NSLLPIANVYY713 pKa = 6.69VHH715 pKa = 6.49KK716 pKa = 10.99VNYY719 pKa = 9.36HH720 pKa = 4.97RR721 pKa = 11.84AKK723 pKa = 10.65NLSSNLKK730 pKa = 10.1NEE732 pKa = 3.93NDD734 pKa = 4.8GILLTQLKK742 pKa = 10.34QSVNDD747 pKa = 3.51NKK749 pKa = 10.76LLSKK753 pKa = 10.2LDD755 pKa = 3.64SMMDD759 pKa = 3.54NPVSRR764 pKa = 11.84HH765 pKa = 4.74VEE767 pKa = 4.32LIHH770 pKa = 6.66IAGFPGCGKK779 pKa = 9.18SHH781 pKa = 7.09PVASLLKK788 pKa = 10.27CKK790 pKa = 7.84TFRR793 pKa = 11.84NSFRR797 pKa = 11.84VVVPTVEE804 pKa = 5.63LRR806 pKa = 11.84SEE808 pKa = 4.07WKK810 pKa = 10.42DD811 pKa = 3.36KK812 pKa = 11.72LEE814 pKa = 4.62LPDD817 pKa = 3.83FEE819 pKa = 4.53SWRR822 pKa = 11.84ISTWEE827 pKa = 3.79TSLLKK832 pKa = 10.26LAKK835 pKa = 10.26VLVIDD840 pKa = 4.86EE841 pKa = 4.58VYY843 pKa = 10.82KK844 pKa = 11.03LPNGFLDD851 pKa = 4.12LAVALDD857 pKa = 3.71PTLQFVILLGDD868 pKa = 4.14PCQGTYY874 pKa = 10.6HH875 pKa = 6.6SLSPHH880 pKa = 5.2STNHH884 pKa = 6.13HH885 pKa = 6.83IYY887 pKa = 10.41PEE889 pKa = 4.03TEE891 pKa = 4.0YY892 pKa = 10.97LSKK895 pKa = 11.18YY896 pKa = 9.93RR897 pKa = 11.84DD898 pKa = 4.6FYY900 pKa = 11.32CLWTHH905 pKa = 6.54RR906 pKa = 11.84TPQVIANLLNVRR918 pKa = 11.84SSSKK922 pKa = 10.89EE923 pKa = 3.35NGQISRR929 pKa = 11.84SNTLNASFPVLATSMAIATHH949 pKa = 6.68LNQNGFRR956 pKa = 11.84SITYY960 pKa = 9.9AGSQGLTLNRR970 pKa = 11.84LTYY973 pKa = 10.63LCVDD977 pKa = 4.27RR978 pKa = 11.84YY979 pKa = 9.9ISLMSPGVTLVALTRR994 pKa = 11.84STKK997 pKa = 10.82GIIFNGNYY1005 pKa = 10.27DD1006 pKa = 3.84LLGNLGYY1013 pKa = 9.53TNTLLEE1019 pKa = 4.54SIFRR1023 pKa = 11.84GEE1025 pKa = 4.67KK1026 pKa = 9.33IDD1028 pKa = 3.9FQHH1031 pKa = 7.23LFHH1034 pKa = 6.12QQLRR1038 pKa = 11.84GSRR1041 pKa = 11.84IITQPIMSPTDD1052 pKa = 2.99RR1053 pKa = 11.84KK1054 pKa = 9.22MLRR1057 pKa = 11.84GGAILITKK1065 pKa = 6.86TAKK1068 pKa = 9.81IDD1070 pKa = 3.49INSSIDD1076 pKa = 3.55FLRR1079 pKa = 11.84QDD1081 pKa = 3.0SQLRR1085 pKa = 11.84SNPEE1089 pKa = 4.63LIPLHH1094 pKa = 7.06DD1095 pKa = 4.56NFYY1098 pKa = 10.5LPPSRR1103 pKa = 11.84LVLSQSFSSSLHH1115 pKa = 6.27PPAKK1119 pKa = 10.17PNNTPFAPTPFEE1131 pKa = 4.02PSYY1134 pKa = 10.37PGYY1137 pKa = 10.0PFEE1140 pKa = 4.92TYY1142 pKa = 9.85FANLPDD1148 pKa = 4.23PSEE1151 pKa = 4.5PEE1153 pKa = 4.52SKK1155 pKa = 10.56EE1156 pKa = 3.33KK1157 pKa = 9.74WYY1159 pKa = 10.59KK1160 pKa = 10.57DD1161 pKa = 3.0KK1162 pKa = 10.99RR1163 pKa = 11.84SQQFPILNKK1172 pKa = 9.91GSPYY1176 pKa = 10.87SPFPANIVAPIHH1188 pKa = 6.85DD1189 pKa = 4.4SKK1191 pKa = 11.46KK1192 pKa = 10.94DD1193 pKa = 3.57STLLPLSINKK1203 pKa = 9.63RR1204 pKa = 11.84LRR1206 pKa = 11.84FRR1208 pKa = 11.84PDD1210 pKa = 2.81NKK1212 pKa = 9.99PYY1214 pKa = 10.59QITEE1218 pKa = 3.89LDD1220 pKa = 3.72HH1221 pKa = 6.72YY1222 pKa = 10.01VANHH1226 pKa = 6.85LFQSYY1231 pKa = 10.48CEE1233 pKa = 4.19TLNTTIDD1240 pKa = 3.11IKK1242 pKa = 11.45VPFDD1246 pKa = 3.07AHH1248 pKa = 7.11LFAEE1252 pKa = 5.28CINLNDD1258 pKa = 3.76YY1259 pKa = 10.56NQLTKK1264 pKa = 9.61KK1265 pKa = 8.78TRR1267 pKa = 11.84ATLVANAYY1275 pKa = 10.2RR1276 pKa = 11.84SDD1278 pKa = 3.94PDD1280 pKa = 3.0WRR1282 pKa = 11.84FTFVRR1287 pKa = 11.84IFSKK1291 pKa = 9.02TQHH1294 pKa = 6.65KK1295 pKa = 10.69INTNSLYY1302 pKa = 10.29TSWKK1306 pKa = 9.38ACQTLALMNDD1316 pKa = 4.87LIVLTLGPVKK1326 pKa = 10.46KK1327 pKa = 10.05YY1328 pKa = 10.43QRR1330 pKa = 11.84LINDD1334 pKa = 4.47PLRR1337 pKa = 11.84KK1338 pKa = 9.92SNIFTYY1344 pKa = 10.39GGKK1347 pKa = 8.06TPFDD1351 pKa = 3.75LSSFCQNHH1359 pKa = 5.55FTPKK1363 pKa = 9.21STVANDD1369 pKa = 3.13YY1370 pKa = 8.79TAFDD1374 pKa = 3.61QSQLGEE1380 pKa = 4.29SLCLEE1385 pKa = 4.11VLKK1388 pKa = 10.21MKK1390 pKa = 10.5RR1391 pKa = 11.84LSIPNNFIEE1400 pKa = 5.02LHH1402 pKa = 5.47LTLKK1406 pKa = 10.76RR1407 pKa = 11.84NLYY1410 pKa = 9.88CQFGPLTCMRR1420 pKa = 11.84FTGEE1424 pKa = 3.96PGTWDD1429 pKa = 4.66DD1430 pKa = 3.68NTDD1433 pKa = 3.48YY1434 pKa = 11.21NLSVLFTLFQITNQAVLVSGDD1455 pKa = 4.04DD1456 pKa = 3.57SCINPIPPFNPRR1468 pKa = 11.84WNSIKK1473 pKa = 10.54KK1474 pKa = 10.2LLLLKK1479 pKa = 10.7FKK1481 pKa = 11.18LEE1483 pKa = 4.0YY1484 pKa = 9.89TKK1486 pKa = 10.77FPIFCGYY1493 pKa = 10.36YY1494 pKa = 9.0VGKK1497 pKa = 10.44AGALRR1502 pKa = 11.84APTALAHH1509 pKa = 6.73KK1510 pKa = 9.43IHH1512 pKa = 6.6NALADD1517 pKa = 3.86DD1518 pKa = 4.93SISDD1522 pKa = 3.34KK1523 pKa = 10.91HH1524 pKa = 7.7LSYY1527 pKa = 10.89LSEE1530 pKa = 3.92FAIGHH1535 pKa = 5.44SLGEE1539 pKa = 4.15QLWTLLPHH1547 pKa = 6.67HH1548 pKa = 6.53EE1549 pKa = 4.4VLAQSALFDD1558 pKa = 3.66YY1559 pKa = 10.3FCRR1562 pKa = 11.84KK1563 pKa = 7.68TSKK1566 pKa = 10.47KK1567 pKa = 10.36NKK1569 pKa = 8.66LLLYY1573 pKa = 9.88VGPPKK1578 pKa = 10.13QEE1580 pKa = 4.05HH1581 pKa = 6.57LNLLTFDD1588 pKa = 3.58IEE1590 pKa = 4.67SYY1592 pKa = 10.19QALRR1596 pKa = 11.84QYY1598 pKa = 10.74QRR1600 pKa = 11.84ANLLSKK1606 pKa = 10.23HH1607 pKa = 5.48QPPPLAPTLEE1617 pKa = 4.38PVLQQIFNNN1626 pKa = 3.93

Molecular weight:
183.98 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1849

223

1626

924.5

104.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.408 ± 1.064

1.46 ± 0.312

4.056 ± 0.008

3.029 ± 0.135

5.138 ± 0.261

3.191 ± 0.158

4.327 ± 0.474

6.057 ± 1.163

4.651 ± 0.067

12.655 ± 1.473

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

0.973 ± 0.149

6.165 ± 0.672

9.627 ± 0.454

4.056 ± 0.366

3.407 ± 0.465

9.519 ± 1.213

6.923 ± 0.258

4.489 ± 0.356

1.19 ± 0.117

3.678 ± 0.573

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski