Phlox virus B

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8II60|A8II60_9VIRU Movement protein TGB2 OS=Phlox virus B OX=475777 PE=3 SV=1
MM1 pKa = 7.85DD2 pKa = 4.15VLVKK6 pKa = 10.24FASDD10 pKa = 3.44YY11 pKa = 11.46GFVRR15 pKa = 11.84LNSKK19 pKa = 9.82LGKK22 pKa = 9.47PIVFHH27 pKa = 6.27CVPGAGKK34 pKa = 9.59SHH36 pKa = 8.11LIRR39 pKa = 11.84KK40 pKa = 9.05LIEE43 pKa = 3.71YY44 pKa = 9.94DD45 pKa = 3.45SRR47 pKa = 11.84FRR49 pKa = 11.84AYY51 pKa = 9.42TLGEE55 pKa = 4.0ADD57 pKa = 3.45QAQLSGVRR65 pKa = 11.84IQALSEE71 pKa = 4.01RR72 pKa = 11.84TTEE75 pKa = 4.13GPYY78 pKa = 10.98DD79 pKa = 4.49LLDD82 pKa = 4.24EE83 pKa = 4.6YY84 pKa = 10.8CQHH87 pKa = 7.27DD88 pKa = 4.41CATEE92 pKa = 4.02GFFAVFGDD100 pKa = 4.42PFQSTTVNHH109 pKa = 6.05LRR111 pKa = 11.84ADD113 pKa = 5.08FICKK117 pKa = 9.51KK118 pKa = 9.36SRR120 pKa = 11.84RR121 pKa = 11.84FGSCTAQLLRR131 pKa = 11.84ALGFDD136 pKa = 4.21VEE138 pKa = 4.64ATGQDD143 pKa = 2.63IVQIKK148 pKa = 9.91GLYY151 pKa = 9.15EE152 pKa = 3.93VDD154 pKa = 3.54PADD157 pKa = 3.37QIIYY161 pKa = 10.23FEE163 pKa = 4.65EE164 pKa = 4.29EE165 pKa = 3.99VGCLLRR171 pKa = 11.84RR172 pKa = 11.84HH173 pKa = 5.57GVEE176 pKa = 3.3ASNIRR181 pKa = 11.84EE182 pKa = 4.2VIGKK186 pKa = 9.08SFNSVTFVTAEE197 pKa = 3.8SKK199 pKa = 10.72IPFEE203 pKa = 3.92SRR205 pKa = 11.84GWVFQCLTRR214 pKa = 11.84HH215 pKa = 5.94RR216 pKa = 11.84EE217 pKa = 3.92SLLILSPDD225 pKa = 3.08ASYY228 pKa = 11.05TSTT231 pKa = 3.56

Molecular weight: 25.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8II63|A8II63_9VIRU 7 kDa protein OS=Phlox virus B OX=475777 PE=3 SV=1
MM1 pKa = 7.95PLTPPPDD8 pKa = 3.47HH9 pKa = 6.46TRR11 pKa = 11.84SLLAVAIGFSIIGIVLVYY29 pKa = 10.54SRR31 pKa = 11.84STLPFTGDD39 pKa = 3.74NIHH42 pKa = 6.42SLPHH46 pKa = 5.73GGLYY50 pKa = 10.15KK51 pKa = 10.89DD52 pKa = 3.55GTKK55 pKa = 10.09QIQYY59 pKa = 9.33GAPRR63 pKa = 11.84KK64 pKa = 9.86LNSLEE69 pKa = 4.32GPQKK73 pKa = 10.76LATQPWAYY81 pKa = 10.98VLLLTLLILISVQVDD96 pKa = 3.32RR97 pKa = 11.84FRR99 pKa = 11.84VCRR102 pKa = 11.84CGVRR106 pKa = 11.84HH107 pKa = 6.1

Molecular weight: 11.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2899

62

2051

483.2

54.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.588 ± 0.772

2.484 ± 0.496

5.243 ± 0.593

7.485 ± 1.288

5.416 ± 0.716

6.83 ± 0.524

2.449 ± 0.264

5.174 ± 0.528

6.933 ± 0.878

8.969 ± 0.835

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.38 ± 0.452

4.105 ± 0.759

4.277 ± 0.586

3.07 ± 0.51

5.347 ± 0.471

7.175 ± 0.278

4.829 ± 0.426

6.554 ± 0.317

1.138 ± 0.185

3.518 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski