Pohorje myodes paramyxovirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae; Jeilongvirus; Myodes jeilongvirus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1GJB1|A0A2P1GJB1_9MONO Nucleocapsid OS=Pohorje myodes paramyxovirus 1 OX=2116604 GN=N PE=3 SV=1
MM1 pKa = 7.62ANFSTSEE8 pKa = 4.05LNQLVQDD15 pKa = 4.82GIKK18 pKa = 9.3TVEE21 pKa = 5.21FIQQATKK28 pKa = 10.78NPKK31 pKa = 7.3EE32 pKa = 4.22TYY34 pKa = 9.78GRR36 pKa = 11.84SAIQKK41 pKa = 9.06PRR43 pKa = 11.84TKK45 pKa = 10.81DD46 pKa = 3.21RR47 pKa = 11.84IKK49 pKa = 10.38AWEE52 pKa = 4.64GVTADD57 pKa = 4.21HH58 pKa = 7.91DD59 pKa = 4.36YY60 pKa = 11.44TGDD63 pKa = 3.66KK64 pKa = 10.36QDD66 pKa = 3.8GVPGKK71 pKa = 10.48EE72 pKa = 3.64KK73 pKa = 10.6GIKK76 pKa = 8.61KK77 pKa = 10.1RR78 pKa = 11.84KK79 pKa = 9.52GKK81 pKa = 10.56GSPKK85 pKa = 10.23DD86 pKa = 3.43NRR88 pKa = 11.84GTGNEE93 pKa = 3.77EE94 pKa = 3.86DD95 pKa = 4.39PIKK98 pKa = 10.6RR99 pKa = 11.84EE100 pKa = 3.58PSTPSEE106 pKa = 4.31AGDD109 pKa = 3.39IQDD112 pKa = 3.94RR113 pKa = 11.84DD114 pKa = 3.81VEE116 pKa = 4.31NTADD120 pKa = 3.67EE121 pKa = 4.19RR122 pKa = 11.84AEE124 pKa = 4.48DD125 pKa = 4.22GGDD128 pKa = 3.34TGSGLHH134 pKa = 5.46QPRR137 pKa = 11.84EE138 pKa = 4.2GSDD141 pKa = 3.12PGSDD145 pKa = 3.4GDD147 pKa = 4.16AEE149 pKa = 4.49SARR152 pKa = 11.84SLGDD156 pKa = 3.5DD157 pKa = 3.4SVSNADD163 pKa = 3.3YY164 pKa = 10.75RR165 pKa = 11.84VILSADD171 pKa = 3.79HH172 pKa = 6.55EE173 pKa = 4.86SSAAEE178 pKa = 4.21TTDD181 pKa = 3.44LPSNVASVRR190 pKa = 11.84PATTEE195 pKa = 3.68DD196 pKa = 3.36FAQIFEE202 pKa = 4.45EE203 pKa = 5.38GSSKK207 pKa = 10.45AHH209 pKa = 6.26RR210 pKa = 11.84RR211 pKa = 11.84LTGVAAYY218 pKa = 8.1TDD220 pKa = 3.27SDD222 pKa = 4.19AAHH225 pKa = 7.04KK226 pKa = 10.79SAGNPVKK233 pKa = 10.6KK234 pKa = 10.7GIDD237 pKa = 3.3GSTASTPSVDD247 pKa = 3.31IQSSGSGAIPSVHH260 pKa = 6.42EE261 pKa = 4.06LLLRR265 pKa = 11.84QPSSHH270 pKa = 5.55AHH272 pKa = 6.16AEE274 pKa = 4.34SAHH277 pKa = 6.3KK278 pKa = 10.3GVPSVSTTGSTYY290 pKa = 10.63KK291 pKa = 10.36SCEE294 pKa = 4.18TASHH298 pKa = 5.59HH299 pKa = 6.67QILEE303 pKa = 4.0GKK305 pKa = 9.8IDD307 pKa = 3.62ILIGNVDD314 pKa = 4.98RR315 pKa = 11.84IASKK319 pKa = 11.11LDD321 pKa = 3.48LLPEE325 pKa = 3.9IKK327 pKa = 10.6EE328 pKa = 4.04EE329 pKa = 3.86IKK331 pKa = 10.99NINKK335 pKa = 10.31KK336 pKa = 8.24ITTLSLGLSTVEE348 pKa = 4.21NYY350 pKa = 9.38IKK352 pKa = 11.04SMMVIIPGSGKK363 pKa = 10.2DD364 pKa = 3.47EE365 pKa = 4.4EE366 pKa = 4.71KK367 pKa = 10.75RR368 pKa = 11.84NPDD371 pKa = 3.24VNPDD375 pKa = 3.45LRR377 pKa = 11.84PVIGRR382 pKa = 11.84DD383 pKa = 3.0NTRR386 pKa = 11.84GLRR389 pKa = 11.84EE390 pKa = 3.88MKK392 pKa = 9.64MEE394 pKa = 4.98RR395 pKa = 11.84GTLEE399 pKa = 4.37DD400 pKa = 5.75LNDD403 pKa = 3.83EE404 pKa = 4.89SNSQPKK410 pKa = 9.49IDD412 pKa = 4.35PKK414 pKa = 11.35YY415 pKa = 10.6LINPLDD421 pKa = 3.89FSKK424 pKa = 11.45SNAANFKK431 pKa = 8.26PTNDD435 pKa = 3.55LASLKK440 pKa = 10.01TIVAMIKK447 pKa = 10.53NEE449 pKa = 4.05VKK451 pKa = 10.67DD452 pKa = 3.17ITTQISLIEE461 pKa = 4.07WVEE464 pKa = 3.93KK465 pKa = 10.83KK466 pKa = 9.88IDD468 pKa = 3.56QLPAEE473 pKa = 4.48EE474 pKa = 4.87VYY476 pKa = 11.62NMVRR480 pKa = 11.84EE481 pKa = 4.34SLDD484 pKa = 3.42GMDD487 pKa = 4.2SDD489 pKa = 4.83EE490 pKa = 4.28SS491 pKa = 3.76

Molecular weight: 53.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1GJA8|A0A2P1GJA8_9MONO TM protein OS=Pohorje myodes paramyxovirus 1 OX=2116604 GN=TM PE=4 SV=1
MM1 pKa = 7.42EE2 pKa = 5.62SKK4 pKa = 10.73LSSLYY9 pKa = 10.19NRR11 pKa = 11.84LRR13 pKa = 11.84RR14 pKa = 11.84TLRR17 pKa = 11.84KK18 pKa = 8.45HH19 pKa = 5.02TGEE22 pKa = 4.06VPSRR26 pKa = 11.84NQEE29 pKa = 3.67PRR31 pKa = 11.84TEE33 pKa = 4.02SRR35 pKa = 11.84PGRR38 pKa = 11.84EE39 pKa = 4.21SQQTMITQEE48 pKa = 4.22TNRR51 pKa = 11.84MEE53 pKa = 4.36CLEE56 pKa = 4.01RR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84EE60 pKa = 4.03LRR62 pKa = 11.84KK63 pKa = 9.84EE64 pKa = 3.51RR65 pKa = 11.84AKK67 pKa = 10.55EE68 pKa = 3.53VLRR71 pKa = 11.84IIEE74 pKa = 4.3EE75 pKa = 4.42LEE77 pKa = 3.89MKK79 pKa = 10.51RR80 pKa = 11.84ILSRR84 pKa = 11.84EE85 pKa = 4.02NPQPPPRR92 pKa = 11.84LAISRR97 pKa = 11.84TGMLKK102 pKa = 9.82ILLMSVQKK110 pKa = 9.82TGEE113 pKa = 4.22IPVLDD118 pKa = 4.42YY119 pKa = 11.17ISLEE123 pKa = 3.88RR124 pKa = 11.84AQILDD129 pKa = 3.49QMEE132 pKa = 4.21MQSLRR137 pKa = 11.84EE138 pKa = 4.2AWVMIQSVMRR148 pKa = 11.84ITVV151 pKa = 2.94

Molecular weight: 17.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

6189

82

2188

687.7

77.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.381 ± 0.651

1.697 ± 0.325

5.865 ± 0.545

5.494 ± 0.388

3.442 ± 0.4

5.526 ± 0.454

2.068 ± 0.345

7.513 ± 0.576

5.704 ± 0.529

8.838 ± 0.874

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.908 ± 0.356

5.93 ± 0.166

5.251 ± 0.957

3.781 ± 0.256

5.251 ± 0.349

8.014 ± 0.52

7.465 ± 1.225

4.863 ± 0.465

0.969 ± 0.151

4.039 ± 0.524
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski