Porphyromonas macacae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Porphyromonadaceae; Porphyromonas

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1843 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A2EJE5|A0A0A2EJE5_9PORP Uncharacterized protein OS=Porphyromonas macacae OX=28115 GN=HQ47_00190 PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 4.73ATSLSEE8 pKa = 3.35ARR10 pKa = 11.84IYY12 pKa = 10.84VGTYY16 pKa = 9.92AKK18 pKa = 10.82YY19 pKa = 10.22NDD21 pKa = 3.72GSIEE25 pKa = 4.15GKK27 pKa = 9.58WFDD30 pKa = 5.19LSDD33 pKa = 4.14FSDD36 pKa = 3.24KK37 pKa = 11.61DD38 pKa = 3.51EE39 pKa = 5.1FYY41 pKa = 10.5EE42 pKa = 4.49ACKK45 pKa = 10.03EE46 pKa = 3.95LHH48 pKa = 6.84ADD50 pKa = 3.91EE51 pKa = 5.33EE52 pKa = 4.63DD53 pKa = 3.73PEE55 pKa = 5.1YY56 pKa = 10.77MFQDD60 pKa = 3.59WEE62 pKa = 4.41NIPDD66 pKa = 3.82EE67 pKa = 5.08LIGEE71 pKa = 4.38SWLSDD76 pKa = 3.27NFFDD80 pKa = 5.86IRR82 pKa = 11.84DD83 pKa = 3.79AMDD86 pKa = 5.13DD87 pKa = 3.57LSEE90 pKa = 4.74DD91 pKa = 3.56EE92 pKa = 4.96QEE94 pKa = 6.35AFMVWCNHH102 pKa = 5.23GSHH105 pKa = 7.29DD106 pKa = 5.51LSTEE110 pKa = 3.97DD111 pKa = 3.42AHH113 pKa = 8.57DD114 pKa = 5.14LISSFRR120 pKa = 11.84DD121 pKa = 3.52DD122 pKa = 3.97FQGKK126 pKa = 8.83YY127 pKa = 9.92NEE129 pKa = 4.43EE130 pKa = 3.26EE131 pKa = 4.14DD132 pKa = 3.62YY133 pKa = 10.92AYY135 pKa = 10.2EE136 pKa = 4.24VVEE139 pKa = 4.01QCYY142 pKa = 9.75EE143 pKa = 3.85LPEE146 pKa = 3.94FAKK149 pKa = 10.18TYY151 pKa = 10.62FDD153 pKa = 3.75YY154 pKa = 11.44EE155 pKa = 4.0KK156 pKa = 10.43FARR159 pKa = 11.84DD160 pKa = 4.15LFMGDD165 pKa = 3.04YY166 pKa = 9.29WFEE169 pKa = 4.77DD170 pKa = 3.65GFVFRR175 pKa = 11.84ASS177 pKa = 3.13

Molecular weight: 21.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A2E1H0|A0A0A2E1H0_9PORP Ferredoxin OS=Porphyromonas macacae OX=28115 GN=HQ47_09300 PE=4 SV=1
MM1 pKa = 7.58LCKK4 pKa = 10.06QHH6 pKa = 6.63FGEE9 pKa = 4.55KK10 pKa = 10.01FSMGRR15 pKa = 11.84DD16 pKa = 3.27RR17 pKa = 11.84FCRR20 pKa = 11.84LLRR23 pKa = 11.84ANDD26 pKa = 3.01LMLRR30 pKa = 11.84KK31 pKa = 9.39RR32 pKa = 11.84RR33 pKa = 11.84LRR35 pKa = 11.84PKK37 pKa = 8.32TTDD40 pKa = 2.6SRR42 pKa = 11.84HH43 pKa = 5.89RR44 pKa = 11.84FHH46 pKa = 8.48CYY48 pKa = 9.74EE49 pKa = 4.82DD50 pKa = 3.91LLNTEE55 pKa = 4.19PKK57 pKa = 10.6LRR59 pKa = 11.84GIQYY63 pKa = 10.66ASTRR67 pKa = 11.84YY68 pKa = 7.17TALLRR73 pKa = 11.84KK74 pKa = 9.2RR75 pKa = 11.84GCRR78 pKa = 11.84ISMTQTGDD86 pKa = 3.61PLHH89 pKa = 6.36NALAEE94 pKa = 4.16RR95 pKa = 11.84MNNTLKK101 pKa = 10.84NSWHH105 pKa = 6.83ISHH108 pKa = 6.83EE109 pKa = 4.14QQSFRR114 pKa = 11.84EE115 pKa = 4.33AEE117 pKa = 4.1LSVEE121 pKa = 4.1RR122 pKa = 11.84AIRR125 pKa = 11.84MYY127 pKa = 11.03NRR129 pKa = 11.84ARR131 pKa = 11.84PHH133 pKa = 5.25QALGAKK139 pKa = 9.08TPMQILDD146 pKa = 3.9SKK148 pKa = 10.43AQNPLLTPEE157 pKa = 4.39EE158 pKa = 4.28EE159 pKa = 4.66LPRR162 pKa = 11.84IAPGLYY168 pKa = 9.8RR169 pKa = 11.84KK170 pKa = 9.16MNALKK175 pKa = 10.0RR176 pKa = 11.84RR177 pKa = 11.84YY178 pKa = 9.18SAGVNPKK185 pKa = 10.11PSS187 pKa = 3.12

Molecular weight: 21.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1843

0

1843

640164

53

2202

347.3

39.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.995 ± 0.049

1.081 ± 0.018

5.313 ± 0.037

6.847 ± 0.052

4.579 ± 0.041

6.764 ± 0.041

2.004 ± 0.027

7.277 ± 0.052

6.787 ± 0.052

9.591 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.759 ± 0.028

4.802 ± 0.041

3.901 ± 0.03

3.32 ± 0.028

5.149 ± 0.043

6.188 ± 0.042

5.207 ± 0.034

6.311 ± 0.037

1.068 ± 0.02

4.052 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski