Saccharomyces 20S RNA narnavirus (ScNV-20S)

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Amabiliviricetes; Wolframvirales; Narnaviridae; Narnavirus

Average proteome isoelectric point is 9.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P25328|RDRP_SCV20 RNA-directed RNA polymerase OS=Saccharomyces 20S RNA narnavirus OX=186772 PE=1 SV=1
MM1 pKa = 7.68KK2 pKa = 10.46EE3 pKa = 3.87PVDD6 pKa = 4.16CRR8 pKa = 11.84LSTPAGFSGTVPPPGRR24 pKa = 11.84TKK26 pKa = 10.44AARR29 pKa = 11.84PGTIPVRR36 pKa = 11.84RR37 pKa = 11.84SRR39 pKa = 11.84GSASALPGKK48 pKa = 10.24IYY50 pKa = 10.22GWSRR54 pKa = 11.84RR55 pKa = 11.84QRR57 pKa = 11.84DD58 pKa = 3.69RR59 pKa = 11.84FAMLLSSFDD68 pKa = 3.67AALAAYY74 pKa = 9.68SGVVVSRR81 pKa = 11.84GTRR84 pKa = 11.84SLPPSLRR91 pKa = 11.84LFRR94 pKa = 11.84AMTRR98 pKa = 11.84KK99 pKa = 8.74WLSVTARR106 pKa = 11.84GNGVEE111 pKa = 4.18FAIASAKK118 pKa = 9.88EE119 pKa = 3.6FSAACRR125 pKa = 11.84AGWISGTVPDD135 pKa = 4.5HH136 pKa = 7.26FFMKK140 pKa = 10.09WLPEE144 pKa = 3.67PVRR147 pKa = 11.84RR148 pKa = 11.84KK149 pKa = 10.1SGLWAQLSFIGRR161 pKa = 11.84SLPEE165 pKa = 4.65GGDD168 pKa = 3.09RR169 pKa = 11.84HH170 pKa = 6.35EE171 pKa = 5.51IEE173 pKa = 5.14ALANHH178 pKa = 6.9KK179 pKa = 10.19AALSSSFEE187 pKa = 4.0VPADD191 pKa = 3.41VLTSLRR197 pKa = 11.84NYY199 pKa = 10.32SEE201 pKa = 3.9DD202 pKa = 2.94WARR205 pKa = 11.84RR206 pKa = 11.84HH207 pKa = 5.84LAADD211 pKa = 4.29PDD213 pKa = 4.17PSLLCEE219 pKa = 4.21PCTGNSATFEE229 pKa = 3.97RR230 pKa = 11.84TRR232 pKa = 11.84RR233 pKa = 11.84EE234 pKa = 3.72GGFAQSITDD243 pKa = 4.3LVSSSPTDD251 pKa = 3.22NLPPLEE257 pKa = 4.51SMPFGPTQGQALPVHH272 pKa = 5.65VLEE275 pKa = 4.8VSLSRR280 pKa = 11.84YY281 pKa = 9.9HH282 pKa = 6.86NGSDD286 pKa = 3.14PKK288 pKa = 11.05GRR290 pKa = 11.84VSVVRR295 pKa = 11.84EE296 pKa = 3.79RR297 pKa = 11.84GHH299 pKa = 5.6KK300 pKa = 9.68VRR302 pKa = 11.84VVSAMEE308 pKa = 3.77THH310 pKa = 6.22EE311 pKa = 4.8LVLGHH316 pKa = 6.83AARR319 pKa = 11.84RR320 pKa = 11.84RR321 pKa = 11.84LFKK324 pKa = 10.41GLRR327 pKa = 11.84RR328 pKa = 11.84EE329 pKa = 3.71RR330 pKa = 11.84RR331 pKa = 11.84LRR333 pKa = 11.84DD334 pKa = 3.27TLKK337 pKa = 11.1GDD339 pKa = 4.37FEE341 pKa = 4.7ATTKK345 pKa = 11.03AFVGCAGTVISSDD358 pKa = 3.26MKK360 pKa = 10.69SASDD364 pKa = 4.96LIPLSVASAIVDD376 pKa = 3.44GLEE379 pKa = 3.82ASGRR383 pKa = 11.84LLPVEE388 pKa = 4.01IAGLRR393 pKa = 11.84ACTGPQHH400 pKa = 6.61LVYY403 pKa = 10.08PDD405 pKa = 3.39GSEE408 pKa = 3.42ITTRR412 pKa = 11.84RR413 pKa = 11.84GILMGLPTTWAILNLMHH430 pKa = 6.89LWCWDD435 pKa = 3.44SADD438 pKa = 3.63RR439 pKa = 11.84QYY441 pKa = 11.59RR442 pKa = 11.84LEE444 pKa = 4.0GHH446 pKa = 6.86PFRR449 pKa = 11.84ATVRR453 pKa = 11.84SDD455 pKa = 3.28CRR457 pKa = 11.84VCGDD461 pKa = 3.98DD462 pKa = 5.4LIGVGPDD469 pKa = 3.06SLLRR473 pKa = 11.84SYY475 pKa = 11.16DD476 pKa = 3.53RR477 pKa = 11.84NLGLVGMILSPGKK490 pKa = 9.72HH491 pKa = 4.72FRR493 pKa = 11.84SNRR496 pKa = 11.84RR497 pKa = 11.84GVFLEE502 pKa = 4.04RR503 pKa = 11.84LLEE506 pKa = 4.08FQTRR510 pKa = 11.84KK511 pKa = 7.36TVYY514 pKa = 8.62EE515 pKa = 3.73HH516 pKa = 6.5AVIYY520 pKa = 10.3RR521 pKa = 11.84KK522 pKa = 9.78VGHH525 pKa = 6.44RR526 pKa = 11.84RR527 pKa = 11.84VPVDD531 pKa = 3.36RR532 pKa = 11.84SHH534 pKa = 7.54IPVVTRR540 pKa = 11.84VTVLNTIPLKK550 pKa = 10.27GLVRR554 pKa = 11.84ASVLGRR560 pKa = 11.84DD561 pKa = 4.72DD562 pKa = 4.49PPVWWAAAVAEE573 pKa = 4.35SSLLSDD579 pKa = 3.85YY580 pKa = 10.39PRR582 pKa = 11.84KK583 pKa = 10.3KK584 pKa = 9.78IFAAARR590 pKa = 11.84TLRR593 pKa = 11.84PGLSRR598 pKa = 11.84QFRR601 pKa = 11.84RR602 pKa = 11.84LGIPPFLPRR611 pKa = 11.84EE612 pKa = 3.97LGGAGLVGPSDD623 pKa = 4.21RR624 pKa = 11.84VDD626 pKa = 3.23APAFHH631 pKa = 7.13RR632 pKa = 11.84KK633 pKa = 9.49AISSLVWGSDD643 pKa = 2.48ATAAYY648 pKa = 10.48SFIRR652 pKa = 11.84MWQGFEE658 pKa = 3.63GHH660 pKa = 6.32PWKK663 pKa = 9.11TAASQEE669 pKa = 4.06TDD671 pKa = 2.49TWFADD676 pKa = 3.76YY677 pKa = 10.92KK678 pKa = 8.56VTRR681 pKa = 11.84PGKK684 pKa = 9.43MYY686 pKa = 9.43PDD688 pKa = 3.43RR689 pKa = 11.84YY690 pKa = 10.6GFLDD694 pKa = 4.04GEE696 pKa = 4.45SLRR699 pKa = 11.84TKK701 pKa = 9.98STMLNSAVYY710 pKa = 8.14EE711 pKa = 4.36TFLGPDD717 pKa = 3.94PDD719 pKa = 3.78ATHH722 pKa = 6.23YY723 pKa = 9.92PSLRR727 pKa = 11.84IVASRR732 pKa = 11.84LAKK735 pKa = 9.74VRR737 pKa = 11.84KK738 pKa = 9.62DD739 pKa = 3.49LVNRR743 pKa = 11.84WPSVKK748 pKa = 10.06PVGKK752 pKa = 10.04DD753 pKa = 2.69LGTILEE759 pKa = 4.41AFEE762 pKa = 4.07EE763 pKa = 4.68SKK765 pKa = 11.25LCTLWVTPYY774 pKa = 10.49DD775 pKa = 3.46ASGYY779 pKa = 10.41FDD781 pKa = 6.02DD782 pKa = 5.96SLLLMDD788 pKa = 4.15EE789 pKa = 4.39SVYY792 pKa = 10.06QRR794 pKa = 11.84RR795 pKa = 11.84FRR797 pKa = 11.84QLVIAGLMRR806 pKa = 11.84EE807 pKa = 4.13GRR809 pKa = 11.84MGDD812 pKa = 3.56LLFPNWLPPSTVVSGFPP829 pKa = 3.46

Molecular weight: 91.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P25328|RDRP_SCV20 RNA-directed RNA polymerase OS=Saccharomyces 20S RNA narnavirus OX=186772 PE=1 SV=1
MM1 pKa = 7.68KK2 pKa = 10.46EE3 pKa = 3.87PVDD6 pKa = 4.16CRR8 pKa = 11.84LSTPAGFSGTVPPPGRR24 pKa = 11.84TKK26 pKa = 10.44AARR29 pKa = 11.84PGTIPVRR36 pKa = 11.84RR37 pKa = 11.84SRR39 pKa = 11.84GSASALPGKK48 pKa = 10.24IYY50 pKa = 10.22GWSRR54 pKa = 11.84RR55 pKa = 11.84QRR57 pKa = 11.84DD58 pKa = 3.69RR59 pKa = 11.84FAMLLSSFDD68 pKa = 3.67AALAAYY74 pKa = 9.68SGVVVSRR81 pKa = 11.84GTRR84 pKa = 11.84SLPPSLRR91 pKa = 11.84LFRR94 pKa = 11.84AMTRR98 pKa = 11.84KK99 pKa = 8.74WLSVTARR106 pKa = 11.84GNGVEE111 pKa = 4.18FAIASAKK118 pKa = 9.88EE119 pKa = 3.6FSAACRR125 pKa = 11.84AGWISGTVPDD135 pKa = 4.5HH136 pKa = 7.26FFMKK140 pKa = 10.09WLPEE144 pKa = 3.67PVRR147 pKa = 11.84RR148 pKa = 11.84KK149 pKa = 10.1SGLWAQLSFIGRR161 pKa = 11.84SLPEE165 pKa = 4.65GGDD168 pKa = 3.09RR169 pKa = 11.84HH170 pKa = 6.35EE171 pKa = 5.51IEE173 pKa = 5.14ALANHH178 pKa = 6.9KK179 pKa = 10.19AALSSSFEE187 pKa = 4.0VPADD191 pKa = 3.41VLTSLRR197 pKa = 11.84NYY199 pKa = 10.32SEE201 pKa = 3.9DD202 pKa = 2.94WARR205 pKa = 11.84RR206 pKa = 11.84HH207 pKa = 5.84LAADD211 pKa = 4.29PDD213 pKa = 4.17PSLLCEE219 pKa = 4.21PCTGNSATFEE229 pKa = 3.97RR230 pKa = 11.84TRR232 pKa = 11.84RR233 pKa = 11.84EE234 pKa = 3.72GGFAQSITDD243 pKa = 4.3LVSSSPTDD251 pKa = 3.22NLPPLEE257 pKa = 4.51SMPFGPTQGQALPVHH272 pKa = 5.65VLEE275 pKa = 4.8VSLSRR280 pKa = 11.84YY281 pKa = 9.9HH282 pKa = 6.86NGSDD286 pKa = 3.14PKK288 pKa = 11.05GRR290 pKa = 11.84VSVVRR295 pKa = 11.84EE296 pKa = 3.79RR297 pKa = 11.84GHH299 pKa = 5.6KK300 pKa = 9.68VRR302 pKa = 11.84VVSAMEE308 pKa = 3.77THH310 pKa = 6.22EE311 pKa = 4.8LVLGHH316 pKa = 6.83AARR319 pKa = 11.84RR320 pKa = 11.84RR321 pKa = 11.84LFKK324 pKa = 10.41GLRR327 pKa = 11.84RR328 pKa = 11.84EE329 pKa = 3.71RR330 pKa = 11.84RR331 pKa = 11.84LRR333 pKa = 11.84DD334 pKa = 3.27TLKK337 pKa = 11.1GDD339 pKa = 4.37FEE341 pKa = 4.7ATTKK345 pKa = 11.03AFVGCAGTVISSDD358 pKa = 3.26MKK360 pKa = 10.69SASDD364 pKa = 4.96LIPLSVASAIVDD376 pKa = 3.44GLEE379 pKa = 3.82ASGRR383 pKa = 11.84LLPVEE388 pKa = 4.01IAGLRR393 pKa = 11.84ACTGPQHH400 pKa = 6.61LVYY403 pKa = 10.08PDD405 pKa = 3.39GSEE408 pKa = 3.42ITTRR412 pKa = 11.84RR413 pKa = 11.84GILMGLPTTWAILNLMHH430 pKa = 6.89LWCWDD435 pKa = 3.44SADD438 pKa = 3.63RR439 pKa = 11.84QYY441 pKa = 11.59RR442 pKa = 11.84LEE444 pKa = 4.0GHH446 pKa = 6.86PFRR449 pKa = 11.84ATVRR453 pKa = 11.84SDD455 pKa = 3.28CRR457 pKa = 11.84VCGDD461 pKa = 3.98DD462 pKa = 5.4LIGVGPDD469 pKa = 3.06SLLRR473 pKa = 11.84SYY475 pKa = 11.16DD476 pKa = 3.53RR477 pKa = 11.84NLGLVGMILSPGKK490 pKa = 9.72HH491 pKa = 4.72FRR493 pKa = 11.84SNRR496 pKa = 11.84RR497 pKa = 11.84GVFLEE502 pKa = 4.04RR503 pKa = 11.84LLEE506 pKa = 4.08FQTRR510 pKa = 11.84KK511 pKa = 7.36TVYY514 pKa = 8.62EE515 pKa = 3.73HH516 pKa = 6.5AVIYY520 pKa = 10.3RR521 pKa = 11.84KK522 pKa = 9.78VGHH525 pKa = 6.44RR526 pKa = 11.84RR527 pKa = 11.84VPVDD531 pKa = 3.36RR532 pKa = 11.84SHH534 pKa = 7.54IPVVTRR540 pKa = 11.84VTVLNTIPLKK550 pKa = 10.27GLVRR554 pKa = 11.84ASVLGRR560 pKa = 11.84DD561 pKa = 4.72DD562 pKa = 4.49PPVWWAAAVAEE573 pKa = 4.35SSLLSDD579 pKa = 3.85YY580 pKa = 10.39PRR582 pKa = 11.84KK583 pKa = 10.3KK584 pKa = 9.78IFAAARR590 pKa = 11.84TLRR593 pKa = 11.84PGLSRR598 pKa = 11.84QFRR601 pKa = 11.84RR602 pKa = 11.84LGIPPFLPRR611 pKa = 11.84EE612 pKa = 3.97LGGAGLVGPSDD623 pKa = 4.21RR624 pKa = 11.84VDD626 pKa = 3.23APAFHH631 pKa = 7.13RR632 pKa = 11.84KK633 pKa = 9.49AISSLVWGSDD643 pKa = 2.48ATAAYY648 pKa = 10.48SFIRR652 pKa = 11.84MWQGFEE658 pKa = 3.63GHH660 pKa = 6.32PWKK663 pKa = 9.11TAASQEE669 pKa = 4.06TDD671 pKa = 2.49TWFADD676 pKa = 3.76YY677 pKa = 10.92KK678 pKa = 8.56VTRR681 pKa = 11.84PGKK684 pKa = 9.43MYY686 pKa = 9.43PDD688 pKa = 3.43RR689 pKa = 11.84YY690 pKa = 10.6GFLDD694 pKa = 4.04GEE696 pKa = 4.45SLRR699 pKa = 11.84TKK701 pKa = 9.98STMLNSAVYY710 pKa = 8.14EE711 pKa = 4.36TFLGPDD717 pKa = 3.94PDD719 pKa = 3.78ATHH722 pKa = 6.23YY723 pKa = 9.92PSLRR727 pKa = 11.84IVASRR732 pKa = 11.84LAKK735 pKa = 9.74VRR737 pKa = 11.84KK738 pKa = 9.62DD739 pKa = 3.49LVNRR743 pKa = 11.84WPSVKK748 pKa = 10.06PVGKK752 pKa = 10.04DD753 pKa = 2.69LGTILEE759 pKa = 4.41AFEE762 pKa = 4.07EE763 pKa = 4.68SKK765 pKa = 11.25LCTLWVTPYY774 pKa = 10.49DD775 pKa = 3.46ASGYY779 pKa = 10.41FDD781 pKa = 6.02DD782 pKa = 5.96SLLLMDD788 pKa = 4.15EE789 pKa = 4.39SVYY792 pKa = 10.06QRR794 pKa = 11.84RR795 pKa = 11.84FRR797 pKa = 11.84QLVIAGLMRR806 pKa = 11.84EE807 pKa = 4.13GRR809 pKa = 11.84MGDD812 pKa = 3.56LLFPNWLPPSTVVSGFPP829 pKa = 3.46

Molecular weight: 91.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

829

829

829

829.0

91.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.565 ± 0.0

1.206 ± 0.0

5.428 ± 0.0

4.463 ± 0.0

4.101 ± 0.0

8.203 ± 0.0

2.292 ± 0.0

3.136 ± 0.0

3.619 ± 0.0

10.374 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.93 ± 0.0

1.568 ± 0.0

6.996 ± 0.0

1.568 ± 0.0

10.253 ± 0.0

8.926 ± 0.0

5.428 ± 0.0

7.479 ± 0.0

2.171 ± 0.0

2.292 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski