Geobacillus sp. CAMR12739

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus; unclassified Geobacillus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1702 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A063YJL0|A0A063YJL0_9BACI Uncharacterized protein OS=Geobacillus sp. CAMR12739 OX=1482738 GN=DI43_18650 PE=4 SV=1
MM1 pKa = 7.28EE2 pKa = 5.7HH3 pKa = 7.27GDD5 pKa = 3.4RR6 pKa = 11.84HH7 pKa = 5.08ITVVDD12 pKa = 3.13EE13 pKa = 4.8HH14 pKa = 8.52GNEE17 pKa = 4.01QLCEE21 pKa = 3.68ILFTFEE27 pKa = 4.68SDD29 pKa = 4.23DD30 pKa = 3.72FGKK33 pKa = 10.65SYY35 pKa = 11.12VFYY38 pKa = 10.84YY39 pKa = 10.0PVGVEE44 pKa = 4.67AEE46 pKa = 4.32DD47 pKa = 3.86EE48 pKa = 4.17NGEE51 pKa = 4.45TEE53 pKa = 4.22VHH55 pKa = 5.84VSAFIPGDD63 pKa = 3.56EE64 pKa = 4.22NKK66 pKa = 10.39EE67 pKa = 4.09GEE69 pKa = 4.44LLPIEE74 pKa = 4.66TEE76 pKa = 4.37EE77 pKa = 3.97EE78 pKa = 3.3WDD80 pKa = 3.66MIEE83 pKa = 4.42EE84 pKa = 4.17VWNTFCAEE92 pKa = 3.99QEE94 pKa = 4.29EE95 pKa = 4.64EE96 pKa = 4.38DD97 pKa = 5.22DD98 pKa = 4.01EE99 pKa = 4.62

Molecular weight: 11.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A063YLP0|A0A063YLP0_9BACI Competence protein ComF OS=Geobacillus sp. CAMR12739 OX=1482738 GN=DI43_17410 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.21QPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.75RR12 pKa = 11.84SKK14 pKa = 9.65VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.3VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1702

0

1702

327887

32

815

192.6

21.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.182 ± 0.087

0.956 ± 0.026

4.841 ± 0.046

7.361 ± 0.076

4.182 ± 0.052

7.133 ± 0.063

2.358 ± 0.039

6.326 ± 0.055

5.55 ± 0.056

9.712 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.799 ± 0.031

3.187 ± 0.044

4.232 ± 0.045

3.653 ± 0.047

6.383 ± 0.069

4.989 ± 0.06

5.035 ± 0.052

7.545 ± 0.064

1.265 ± 0.028

3.309 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski