Blackberry virus E

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Allexivirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8VAG3|F8VAG3_9VIRU Coat protein OS=Blackberry virus E OX=1043184 PE=4 SV=1
MM1 pKa = 7.67APLSVDD7 pKa = 4.78FFNQAHH13 pKa = 5.94TYY15 pKa = 9.39VVQLLEE21 pKa = 4.79RR22 pKa = 11.84IHH24 pKa = 6.71NNTIVPMGRR33 pKa = 11.84TVNRR37 pKa = 11.84IGGEE41 pKa = 3.8LDD43 pKa = 2.9QGLRR47 pKa = 11.84DD48 pKa = 3.65INAKK52 pKa = 10.26LDD54 pKa = 3.89VITSQTASAPATTVDD69 pKa = 4.29DD70 pKa = 3.97ARR72 pKa = 11.84QIPIEE77 pKa = 4.63GPASPTLTGNPLIQQTPSATRR98 pKa = 11.84FFSDD102 pKa = 3.0SNAVLSEE109 pKa = 4.04TQRR112 pKa = 11.84ITRR115 pKa = 11.84ILPSNTFPMQPSTLPTDD132 pKa = 3.53EE133 pKa = 6.19LFGQLHH139 pKa = 6.77ALHH142 pKa = 6.46QNLLQWSIDD151 pKa = 2.82IDD153 pKa = 3.66NRR155 pKa = 11.84VQQLASSVNAKK166 pKa = 10.17LDD168 pKa = 3.52AVTFNSANQVSTATLNRR185 pKa = 11.84LLEE188 pKa = 4.25EE189 pKa = 4.03LHH191 pKa = 7.51ANMATSQRR199 pKa = 11.84TGEE202 pKa = 4.13AAQTMLEE209 pKa = 4.15HH210 pKa = 6.75LADD213 pKa = 3.42IQRR216 pKa = 11.84RR217 pKa = 11.84LPSTAPSDD225 pKa = 3.92ALQQLIQLLSTNRR238 pKa = 11.84PVEE241 pKa = 4.2EE242 pKa = 4.29TTPTATPHH250 pKa = 6.27SEE252 pKa = 4.14LPALNPVHH260 pKa = 6.45NALRR264 pKa = 11.84CRR266 pKa = 11.84TYY268 pKa = 11.35GSIEE272 pKa = 4.08FNGQTFKK279 pKa = 11.24RR280 pKa = 11.84PIDD283 pKa = 3.75FVGRR287 pKa = 11.84PPSTTLHH294 pKa = 6.05LRR296 pKa = 11.84LDD298 pKa = 3.48VRR300 pKa = 11.84RR301 pKa = 11.84AADD304 pKa = 3.15HH305 pKa = 5.87TAVHH309 pKa = 6.15FTLHH313 pKa = 6.64DD314 pKa = 4.15LDD316 pKa = 4.67RR317 pKa = 11.84VLLEE321 pKa = 4.12QVIQTPHH328 pKa = 5.91QLQHH332 pKa = 6.83LPDD335 pKa = 4.94DD336 pKa = 4.24ALFLINLHH344 pKa = 6.07CPRR347 pKa = 11.84FTYY350 pKa = 10.09RR351 pKa = 11.84RR352 pKa = 11.84EE353 pKa = 4.16AGCC356 pKa = 4.32

Molecular weight: 39.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8VAG2|F8VAG2_9VIRU Serine-rich p40 protein OS=Blackberry virus E OX=1043184 PE=4 SV=1
MM1 pKa = 7.54SFTPPPDD8 pKa = 3.42FSKK11 pKa = 11.24VYY13 pKa = 9.15LAAVAGMCATLAIHH27 pKa = 6.25IQISSRR33 pKa = 11.84RR34 pKa = 11.84PHH36 pKa = 7.14VGDD39 pKa = 4.6NIHH42 pKa = 6.52HH43 pKa = 6.77LPHH46 pKa = 6.72GGHH49 pKa = 5.67YY50 pKa = 10.0QDD52 pKa = 3.3GNKK55 pKa = 10.06RR56 pKa = 11.84IIYY59 pKa = 10.06AGSHH63 pKa = 5.81YY64 pKa = 10.93SNQLQTGDD72 pKa = 2.87HH73 pKa = 6.11WAAILVAVLILAILVSEE90 pKa = 4.41RR91 pKa = 11.84CLRR94 pKa = 11.84PSNRR98 pKa = 11.84SCPHH102 pKa = 5.11HH103 pKa = 6.53HH104 pKa = 6.96

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2442

104

1499

488.4

54.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.681 ± 1.109

1.392 ± 0.349

5.651 ± 0.561

5.201 ± 0.699

3.931 ± 0.229

4.627 ± 0.361

3.89 ± 0.648

4.382 ± 0.426

4.545 ± 1.214

10.36 ± 0.797

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.597 ± 0.241

4.545 ± 0.662

7.043 ± 0.724

5.119 ± 0.565

5.324 ± 0.481

6.224 ± 0.659

7.944 ± 0.741

5.856 ± 0.299

0.696 ± 0.116

2.989 ± 0.511
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski